The pathological tissues were obtained by surgery, and fixed with 10% formaldehyde and then embedded in paraffin and sliced at a thickness of 4 µm. For hematoxylin and eosin (H&E) staining, paraffin-embedded thyroid tissue sections were deparaffinized with xylene for 5 min (this process was repeated once), 100% ethanol for 5 min, 90% ethanol for 2 min, 80% ethanol for 2 min, 70% ethanol for 2 min, and washed with distilled water for 2 min. The sections (4-µm-thick) were stained with hematoxylin stain (Beyotime Institute of Biotechnology, Inc.) for 5 min and washed with distilled water for 10 min at room temperature. The sections were differentiated with acid alcohol slow differentiation solution (Beyotime Institute of Biotechnology, Inc.) for 30 sec and soaked in distilled water for 15 min. The sections were stained with eosin stain (Beyotime Institute of Biotechnology, Inc.) for 2 min at room temperature. Slides were viewed with a Nikon Eclipse 80i microscope equipped with a digital camera (DS-Ri1; Nikon Corp.).
Eosin stain
Manufactured by Beyotime
Sourced in China
Eosin stain is a laboratory reagent used in histology and cytology for staining biological samples. It is a pink/red dye that binds to basic structures within cells, providing contrast and highlighting cellular features for microscopic examination.
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Lab products found in correlation
2 protocols using eosin stain
1
Histological Analysis of Thyroid Tissue
2
Histological Processing of Rat Myocardial Tissue
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After collecting the rat heart, we used PBS to wash away excess blood from the heart. Then, we used 4% paraformaldehyde to fix myocardial tissue for 24 h. The fixed tissue can be stored in PBS for a long time. We put myocardial tissue into gradient alcohol in order to dehydrate. Then, we put myocardial tissue in xylene and paraffin solution to make paraffin blocks. The microtome is used to make paraffin sections with the thickness of 5 μm. We put paraffin sections in a 37°C incubator for 3 d. Then, we put the paraffin sections in xylene solution and gradient alcohol sequentially. After washing the sections with running water, we stained the cell nucleus with hematoxylin stain (Beyotime, Shanghai, China). Then, we put the sections in 1% hydrochloric acid alcohol for 3 s and quickly rinsed the sections with running water. Then, we put the sections in eosin stain (Beyotime, Shanghai, China) and gradient alcohol in turn for 3 min each time. Finally, we use neutral gum for mounting and observe the staining results using an optical microscope.
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