Gfap cre ert2

We created the Cyp19a1 Cre/LoxP conditional KO mice with an inducible GFAP promoter to conditionally inactivate the aromatase in their astrocytes. GFAP-Cre/ERT2 (Jackson lab, Bar Harbor, ME, USA. stock no. 012849) mice were crossed with Cyp19a1 flox/flox animals, which had LoxP sites surrounding exons 10 and 11 of the Cyp19a1 gene. Using the methods previously reported, Cyp19a1 flox/flox (FLOX) mice were produced [34 (link)]. We confirmed the genotyping of the animals by a genomic DNA PCR, using the following primers: FLOX-forward: 5′-GCCATATTCCTGCAACAGTTTATTTGAGG-3′; FLOX-reverse: 5′-GTAAAACCATTCCTGGAAAATTCATAACAACC-3′; CreERT2-forward: 5′-GGCCAGGCTGTTCTTCTTAG-3′; CreERT2-reverse: 5′-ATACCGGAGATCATGCAAGC-3′; Internal positive control forward: 5′-CTAGGCCACAGAATTGAAAGATCT-3′; and Internal positive control reverse: 5′-GTAGGTGGAAATTCTAGCATCATCC-3′. The mice with the genotype of Cyp19a1flox/flox; GFAPCreERT2 were identified as the GFAP-ARO-iKO mice. All the animal experiments were approved and authorized by the Institutional Animal Care and Use Committees of Augusta University and UT Health San Antonio.

NestinCreER^T2 (Jackson ID #016261), floxed Ednrb (Jackson ID #011080), PdgfraCreER^T2 (Jackson ID #018280), Rosa26YFP (Jackson ID #006148), GFAPCreER^T2 (Jackson ID #012849), and C57bl/6n (Jackson ID #000664) mice were purchased from the Jackson Laboratory. Floxed ET-1 mice were obtained from Dr. Ralph Shohet at the University of Hawaii as previously described^{15 (link)}. All mouse colonies were maintained in the animal facility of Children’s National Hospital, under standard conditions (65–75 °C, 40–60% humidity, 12 h light/dark cycles). All animal procedures were performed ethically, and according to the Institutional Animal Care and Use Committee of the Children’s National Hospital (protocol #30578) and the Guide for the Care and Use of Laboratory Animals (National Institutes of Health).