Fmoc nh peg nh2

α-Amino-ω-methoxy poly(ethylene glycol) (mPEG-NH₂, M_w = 10,000 g mol-1, M_w/M_n =1.05) and Fmoc-NH-PEG-NH₂, M_w = 10,000 g mol-1 and mPEG-NH_2, M_w = 5,000 g mol⁻¹ were purchased from JenKem Technology (TX, USA). L-leucine and D, L-leucine (Leu), 1, 2-ethylenediamine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), paclitaxel, and other chemicals were purchased from Sigma-Aldrich (St Louis, MO) and were used without further purification. Trastuzumab was obtained from UNMC pharmacy. Fetal bovine serum (FBS), DMEM and RPMI 1640 medium, penicillin, streptomycin, Trypsin–ethylenediaminetetraacetic acid (EDTA) (0.05% trypsin, 5.3 mM EDTA tetra-sodium) and other chemicals were purchased from Invitrogen (Carlsbad, CA, USA). MTT reagent (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) was purchased from Research Products International (Prospect, IL). Cyanine3 (Cy3) carboxylic acid was purchased from Lumiprobe Life Science Solutions. All other chemicals were of reagent grade and used without further purification.

α-Amino-ω-methoxy poly(ethylene glycol) (mPEG-NH₂, Mw = 5000 g mol⁻¹, M_w/M_n = 1.05) was purchased from Creative PEGWorks Inc., (NC, USA). Fmoc-NH-PEG-NH₂ (M_w = 7500 g mol⁻¹, Mw/Mn = 1.04) was purchased from JenKem technology (TX, USA). CDDP was purchased from Acros Organics (NJ, USA). L-Glutamic acid γ-benzyl ester (BGlu), L-phenylalanine (Phe), 1,2-ethylenediamine (ED), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), paclitaxel, folic acid and other chemicals were purchased from Sigma-Aldrich (MO, USA) and were used without further purification. Fetal bovine serum (FBS), RPMI 1640 medium, penicillin, streptomycin, Trypsin–ethylenediaminetetraacetic acid (EDTA) (0.5% trypsin, 5.3 mM EDTA tetra-sodium) and other chemicals were purchased from Invitrogen (CA, USA).