Images of axonal growth cones were imaged by Nikon A1R laser scanning confocal microscopy with a Plan Apo VC 60× oil objective with numerical aperture 1.4. Brain slices were imaged by Neurolucida with Plan Apo 10× objective or Nikon A1R with a Plan Apo 10× objective. For colocalization analysis, the Pearson’s correlation coefficient and % voxels of colocalization were obtained from both channels with ImageJ plugin “Colocalization analysis—colocalization threshold.” Data analysis was performed with ANOVA with post hoc or Student’s t test. Errors bars in graphs represent SEM.
Plan apo 10 objective
Manufactured by Nikon
The Plan Apo 10× objective is a high-quality optical lens designed for use in various laboratory applications. It provides a 10× magnification and is part of Nikon's plan apochromatic series, known for their superior optical performance and accurate color reproduction. The lens is optimized for flatness of field and is suitable for a range of microscopy techniques.
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3 protocols using plan apo 10 objective
1
Axonal Growth Cone Imaging
2
Quantitative Analysis of Pluripotent Cell Populations
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Confocal images were acquired using an A1 confocal microscope (Nikon) using a Plan Apo 10× objective at 1-μm increments. Figure 2 D was generated from auto-stitching three confocal stacks and using maximum projection for each channel at 10× (Nikon Elements). For population and ISL1 nuclei analysis, three representative sites per Chip were acquired in 3 chips per condition over three experiments. Images were then cropped to only top channel using IMARIS software. GFP-positive nuclei were quantified for each site using a spots algorithm and averaged per Chip. ISL1-positive nuclei were quantified by filtering GFP-positive data by ISL1 co-localization. Population statistics were determined by one-way ANOVA using PRISM (GraphPad).
3
Quantifying Malaria Parasite Transmission
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An. stephensi mosquitoes infected with Pf-ef1α-tdTomato, Pf-peg4-tdTomato or Pf-csp-GFP parasites were maintained at 25 °C, 80% humidity and on days 10 and 14 after the infective-blood meal, mosquitoes were dissected and midguts or salivary glands were harvested for sporozoite counts. On day 10 midguts were observed and photographed for oocyst counts by fluorescence and phase microscopy using an upright Nikon E600 microscope with a PlanApo 10× objective. On day 14, salivary glands from 30–50 mosquitoes were pooled, and released sporozoites were counted using a haemocytometer. For direct visualization of Pf-ef1α-tdTomato or Pf-peg4-tdTomato sporozoites in situ, infected mosquitoes were immobilized at 4 °C and transferred to a petri dish pre-chilled on ice. Mosquitoes were observed under an Olympus SZX7 fluorescence stereo microscope for detection of red fluorescence from transgenic sporozoites that had invaded the salivary glands inside the mosquito thoracic cavity. Images of the mosquitoes were obtained using the Q-Capture Pro 7 software. Uninfected mosquitoes were used as negative control.
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