Artemether

Manufactured by Merck Group

Sourced in United States

Artemether is a pharmaceutical compound used as a labeling agent. It is a semi-synthetic derivative of artemisinin, a natural compound extracted from the Artemisia annua plant. Artemether is commonly used in various laboratory applications, such as cell staining and fluorescence microscopy, due to its light-absorbing and fluorescent properties.

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Lab products found in correlation

6 protocols using artemether

Lateral Flow Assay for Antimalarial Drugs

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Carboxylate-modified TRFN, 1% solids, 200 nm, 580/605 (λ_ex/λ_em), and CGN, 30 nm, were purchased from Shanghai Jieyi Biotechnology. Three haptens of artemether, dihydroartemisinin, and artesunate conjugated with bovine serum albumin (BSA), three monoclonal antibodies (mAbs) against artemether, dihydroartemisinin, and artesunate were produced in our laboratory [11 (link), 12 (link), 15 (link)].
1-Ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC) and goat anti-mouse IgG were purchased from Sigma (St. Louis, MO, USA). Artemether, dihydroartemisinin and artesunate standards were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The nitrocellulose filter membrane (NC membrane), sample pad, conjugate pad, absorbance pad, support plate, the portable optical scanner, and the fluorescence lateral flow reader were obtained from Shanghai Jinbiao Biotechnology Co., Ltd. (Shanghai, China). The other chemicals and organic solvents used were of analytical grade and purchased from Sinopharm Chemical Reagent (Beijing, China). The commercial antimalarial drugs used for detection were collected from Africa and Southeast Asia.

Qian J., He Q., Liu L., Wang M., Wang B, & Cui L. (2020). Rapid quantification of artemisinin derivatives in antimalarial drugs with dipstick immunoassays. Journal of pharmaceutical and biomedical analysis, 191, 113605.

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Anti-malarial Compounds Characterization

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DSM267 and DSM265 were gifts from M. Phillips from University of Texas (UT) Southwestern (35 (link)). l-dihydroorotic acid (DHO) was purchased from Sigma-Aldrich (St. Louis, MO). Amodiaquine, artemether, atovaquone, chloroquine, dihydroartemisinin, and mefloquine were purchased from Sigma-Aldrich (St. Louis, MO).

Mandt R.E., Lafuente-Monasterio M.J., Sakata-Kato T., Luth M.R., Segura D., Pablos-Tanarro A., Viera S., Magan N., Ottilie S., Winzeler E.A., Lukens A.K., Gamo F.J, & Wirth D.F. (2019). In vitro selection predicts malaria parasite resistance to dihydroorotate dehydrogenase inhibitors in a mouse infection model. Science translational medicine, 11(521), eaav1636.

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Neuroblastoma Cell Line Transfection

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Human neuroblastoma cell lines SK-N-SH, SH-SY5Y, and SK-N-BE2 were purchased from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in an atmosphere containing 5% CO₂ at 37°C. Artemether and doxorubicin were purchased from Sigma-Aldrich (St. Louis, MO, USA). For transfection, neuroblastoma cells were transiently transfected with the recombinant plasmid pcDNA(−)3.1-B7-H3 or control pcDNA(−)3.1. At 48 h after transfection, the transfection efficiency was evaluated by real-time PCR and Western blot analysis.

Tan W.Q., Chen G., Ye M, & Jia B. (2017). Artemether Regulates Chemosensitivity to Doxorubicin via Regulation of B7-H3 in Human Neuroblastoma Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 4252-4259.

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Artemether Dilution and Storage

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Artemether (80 mg·mL⁻¹) were obtained from Sigma‐Aldrich Co. (St Louis, MO, USA) and dissolved in 80 mL of sterile water to form 10 mg·mL⁻¹ storage solution conserved at 4 °C and then diluted to the required concentration with DMEM (4500 mg·L⁻¹ glucose, 10% fetal bovine serum, 1% penicillin/streptomycin) when used.

Wu J., Li L., Wang Y., Ren X., Lin K, & He Y. (2019). The HSP90/Akt pathway may mediate artemether‐induced apoptosis of Cal27 cells. FEBS Open Bio, 9(10), 1726-1733.

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Artemether and Sulfadiazine Cytotoxicity Assay

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Artemether (methyl-ether-qinghaosu) was purchased from Exim Pharm Co. (USA). Artemether was prepared in ethanol–water (v/v) (30–70%) with 1000 µg/ml concentration, then 5, 10, 25, 50, and 100 µg/ml dilutions were made from stock solutions in DMEM.[18 ] Sulfadiazine was obtained from Sigma (Sigma-Aldrich) and solved in DMSO (dimethyl sulfoxide) as stock solutions with 1000 µg/ml concentration. Following 1.6, 3.12, 6.25, 12.5, 25, 50, 100, and 200 µg/ml dilutions were prepared in DMEM. Provided solutions were stored at 4°C and used in cytotoxicity assays and in vitro experiments.[1 (link)]

Mikaeiloo H., Ghaffarifar F., Dalimi A., Sharifi Z, & Hassan Z.M. (2016). Apoptotic activity and anti-Toxoplasma effects of artemether on the tachyzoites and experimental infected Vero and J774 cell lines by Toxoplasma gondii. Indian Journal of Pharmacology, 48(2), 179-185.

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Artemether Modulation of LPS Response

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Artemether (Sigma) was dissolved in dimethyl sulphoxide (DMSO) to a concentration of 0.1 M and aliquots stored in -80°C. BAY 11-7085 and SKF 86002 dihydrochloride were purchased from Tocris. In all experiments, cells were treated with Artemether
(5-40 µM) in the absence or presence of LPS (1 µg/ml).

Okorji U.P., Velagapudi R., El-Bakoush A., Fiebich B.L, & Olajide O.A. (2016). Antimalarial Drug Artemether Inhibits Neuroinflammation in BV2 Microglia Through Nrf2-Dependent Mechanisms. Molecular neurobiology, 53(9).

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