Blood samples were kept on ice and centrifuged (1000 ×g, 15 min) within 2–4 h after collection. Plasma and serum aliquots were kept at -20°C until analyses were performed. Plasma glucose, cholesterol, and triglycerides were determined by standard methods. Serum levels of C-reactive protein (CRP) were assessed using a CRP Human Instant ELISA kit (eBioscience, San Diego, CA, United States), and those of malondialdehyde (MDA) with a colorimetric assay of lipid peroxidation (Bioxytech LPO-586, Oxis International SA, Paris, France); the within-run coefficient of variation ranged from 1.2 to 3.4%, depending on the concentration of MDA (Gerard-Monnier et al., 1998 (link)). Serum leptin was measured by a sensitive ELISA test (Human Leptin ELISA Development Kit, PeproTech Inc., Rocky Hill, CT, United States); the detectable concentration range was 63–4000 pg/mL and the intra-assay and inter-assay coefficients of variation were 5.21 and 5.20%, respectively.
Crp human instant elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CRP Human Instant ELISA kit is a rapid immunoassay designed for the quantitative determination of C-reactive protein (CRP) in human serum and plasma samples. The kit provides a simple, straightforward procedure for measuring CRP levels.
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Lab products found in correlation
3 protocols using crp human instant elisa kit
1
Biomarker Assays in Plasma and Serum
2
Fasting Blood Biomarker Analysis
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Fasting blood samples were drawn by venepuncture after a 12 h fast and collected in separate tubes for serum and plasma. Samples were kept on ice and centrifuged (1000× g, 15 min) within 2–4 h after collection. Plasma and serum aliquots were kept at −20 °C until analyses were performed. Plasma glucose, cholesterol, and triglycerides were determined by standard methods. Serum levels of CRP were determined by using a CRP Human Instant ELISA kit (Ebioscience, San Diego, CA, USA), and those of MDA with a colorimetric assay of lipid peroxidation (Byoxytech LPO-586, Oxis International S.A., Paris, France); the within-run coefficient of variation ranged from 1.2% to 3.4%, depending on the concentration of MDA [31 (link)].
Serum leptin was measured by a sensitive ELISA test (Human Leptin ELISA Development Kit, 900-K90 PeproTech Inc., Rocky Hill, NJ, USA) according to the manufacturer’s instructions. The detectable concentration range was 63–4000 pg/mL. The intra-assay and interassay coefficients of variation were 5.21% and 5.20%, respectively.
Serum leptin was measured by a sensitive ELISA test (Human Leptin ELISA Development Kit, 900-K90 PeproTech Inc., Rocky Hill, NJ, USA) according to the manufacturer’s instructions. The detectable concentration range was 63–4000 pg/mL. The intra-assay and interassay coefficients of variation were 5.21% and 5.20%, respectively.
3
Measurement of Serum Antioxidant and Oxidative Stress
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A blood sample was drawn by venepuncture after approximately 12-hour fast and collected in separate tubes for serum and plasma. Samples were kept on ice and centrifuged (1000 × g, 15 minutes) within 2-4 hours after collection. Plasma and serum aliquots were storage at -20 °C until analyses were performed. Serum glucose, serum total cholesterol, serum HDL-cholesterol, serum LDL-cholesterol and serum triglycerides were determined by using an automated biochemical auto-analyser.
Total antioxidant capacity (TAC) in serum was determined by the colorimetric assay P40117 (Innoprot, Innovative Technologies in Biological Systems, Vizcaya, Spain). This method determines the conversion of, Cu 2+ to Cu + by serum small molecules and proteins. The reduced ion is chelated with a colorimetric probe, giving a broad absorbance peak around 450 nm, which is proportional to the TAC [31] (link). Serum malondialdehyde (MDA) concentrations were determined by the spectrophotometric method of lipid peroxidation LPO-586 (Byoxytech, Oxis International, Portland, OR) [32] (link). Serum levels of C-reactive protein (CRP) were determined by CRP Human Instant ELISA kit (eBioscience, San Diego, CA).
Total antioxidant capacity (TAC) in serum was determined by the colorimetric assay P40117 (Innoprot, Innovative Technologies in Biological Systems, Vizcaya, Spain). This method determines the conversion of, Cu 2+ to Cu + by serum small molecules and proteins. The reduced ion is chelated with a colorimetric probe, giving a broad absorbance peak around 450 nm, which is proportional to the TAC [31] (link). Serum malondialdehyde (MDA) concentrations were determined by the spectrophotometric method of lipid peroxidation LPO-586 (Byoxytech, Oxis International, Portland, OR) [32] (link). Serum levels of C-reactive protein (CRP) were determined by CRP Human Instant ELISA kit (eBioscience, San Diego, CA).
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