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Mouse leptin elisa kit

Manufactured by Crystal Chem
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The Mouse Leptin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse leptin levels in serum, plasma, and other biological fluids. The kit utilizes a specific antibody coated on a 96-well plate to capture the target analyte, which is then detected using a second antibody conjugated with an enzyme. Upon addition of a substrate, the enzyme catalyzes a color change reaction, allowing for colorimetric detection and quantification of the target analyte.

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Lab products found in correlation

Ultra sensitive mouse insulin elisa kit, by Crystal Chem (20 mentions) Mouse adiponectin elisa kit, by Crystal Chem (8 mentions) Mouse insulin elisa kit, by Crystal Chem (6 mentions) Rat insulin elisa kit, by Crystal Chem (4 mentions) Glucometer elite, by Bayer (3 mentions) Mouse adiponectin elisa kit, by Linco Research (3 mentions) Mouse glucose assay, by Crystal Chem (2 mentions) Triglyceride quantification assay kit, by Abcam (2 mentions) Heparinized capillary tube, by Thermo Fisher Scientific (2 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (2 mentions) Pentra 400 analyzer, by Horiba (2 mentions) Mouse ultrasensitive insulin elisa, by ALPCO (2 mentions) Free glycerol reagent, by Merck Group (2 mentions) Mouse insulin elisa, by Mercodia (1 mentions) Milliplex map mouse metabolic hormone magnetic bead panel, by Merck Group (1 mentions) Mouse rat gdf15 quantikine elisa kit, by R&D Systems (1 mentions) Spectrostar nano microplate reader, by BMG Labtech (1 mentions) Hdl cholesterol precipitating reagent, by Horiba (1 mentions) Ultrasensitive rat insulin elisa, by Crystal Chem (1 mentions) Onetouch ultra blood glucose meter, by LifeScan (1 mentions)

Close spelling variants of this product

Leptin (27 citations) Mouse Leptin ELISA (23 citations) Mouse ELISA kit (18 citations) Leptin ELISA kit (5 citations) ELISAs (3 citations)

65 protocols using mouse leptin elisa kit

1

Serum Biomarker Quantification in Mice

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Mouse Insulin ELISA (Cat # 10‐1247‐01, Mercodia, Uppsala, Sweden), mouse Adiponectin ELISA kit (Cat #: 80569, Crystal Chem, IL, USA), and mouse Leptin ELISA kit (Cat #: 90030, Crystal Chem) were used to determine insulin, adiponectin, and leptin amounts in the serum of WT and CD169‐DTR mice following the manufacturer’s instructions.
Chen Q., Lai S.M., Xu S., Tan Y., Leong K., Liu D., Tan J.C., Naik R.R., Barron A.M., Adav S.S., Chen J., Chong S.Z., Ng L.G, & Ruedl C. (2021). Resident macrophages restrain pathological adipose tissue remodeling and protect vascular integrity in obese mice. EMBO Reports, 22(8), e52835.
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2

Measuring Leptin and Ova-specific IgE in Asthmatic Mice

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Mouse Leptin ELISA kit (#90030, Crystal Chem) was utilized for leptin measurement following the manufacturer’s instruction. To measure Ova-specific IgE, plate-bound Ova (100 μg ml−1) were used as capture and anti-mouse IgE (23G3, eBioscience) as detection antibody. LLN cells (4 × 106 cells ml−1) from the asthmatic HFD and ND mice were recalled with various concentrations of Ova for 3 days and the supernatants were collected for measurement of cytokine expression by ELISA using a standard protocol. For in vitro differentiated TH2 cells, the cells were starved for 24 h and transfected with siXbp1 or scramble siRNA. The resulting cells were washed and treated with or without leptin (200 ng ml−1) for 6 h, and finally the supernatants were collected and used for measuring cytokines expression by ELISA.
Zheng H., Wu D., Wu X., Zhang X., Zhou Q., Luo Y., Yang X., Chock C.J., Liu M, & Yang X.O. (2018). Leptin Promotes Allergic Airway Inflammation through Targeting the Unfolded Protein Response Pathway. Scientific Reports, 8, 8905.
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3

Glucose Tolerance and Metabolic Testing

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Glucose tolerance tests (GTTs) were performed on all mice at approximately 17 weeks of age. Following a 12‐hr fast, where food was removed and alcohol was replaced with water, a small prick was made at the tip of the mouse's tail and a baseline blood glucose level (Time 0) was determined using One‐Touch Ultra‐2 glucose monitors. An intraperitoneal injection of 2 g/kg glucose was given to each mouse and blood glucose levels were subsequently measured at 30, 60, and 120 min postinjection. Four‐hour fasting insulin and leptin levels were measured after 18 weeks of age. Blood was collected from each mouse and allowed to clot and was centrifuged at 4°C for 20 min at 2000 g in order to obtain serum. ELISAs for insulin (Ultra Sensitive Mouse Insulin ELISA Kit, Crystal Chem Inc., Downers Grove, IL, USA) and leptin (Mouse Leptin ELISA Kit; Crystal Chem) were conducted for both male and female mice.
Gelineau R.R., Arruda N.L., Hicks J.A., Monteiro De Pina I., Hatzidis A, & Seggio J.A. (2017). The behavioral and physiological effects of high‐fat diet and alcohol consumption: Sex differences in C57BL6/J mice. Brain and Behavior, 7(6), e00708.
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4

Plasma Metabolic Biomarkers Evaluation

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Plasma glucose was evaluated with GOD-PAP Kit (Biolabo, Maizy, France). Concentrations of plasma non-esterified fatty acids (NEFA) were determined in accordance with the ACS-ACOD Method (NEFA-HR2; Wako, Osaka, Japan). Plasma insulin was assayed with an ultra-sensitive mouse insulin enzyme-linked immunoassay kit (ELISA) kit (CrystalChem, Downers Grove, IL, USA). Levels of plasma leptin were measured with a mouse leptin ELISA Kit (Crystal Chem).
Sen S., Dumont S., Sage-Ciocca D., Reibel S., de Goede P., Kalsbeek A, & Challet E. (2018). Expression of the clock gene Rev-erbα in the brain controls the circadian organisation of food intake and locomotor activity, but not daily variations of energy metabolism. Journal of neuroendocrinology, 30(1).
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5

Plasma Biomarker Quantification

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Plasma leptin and glucose levels were determined using Mouse Leptin ELISA kit (Crystal Chem) and Mouse Glucose Assay (Crystal Chem), respectively. TAG level in the plasma was measured using Triglyceride Quantification Assay kit (Abcam).
Boscaini S., Cabrera-Rubio R., Speakman J.R., Cotter P.D., Cryan J.F, & Nilaweera K.N. (2019). Dietary α-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed mice. The British journal of nutrition, 121(10).
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6

Metabolic Markers in Mouse Models

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Plasma leptin and glucose levels were determined using Mouse Leptin ELISA kit (Crystal Chem, USA) and Mouse Glucose Assay (Crystal Chem, USA), respectively. Mouse Insulin ELISA Kits, Ultra‐Sensitive Assay (Crystal Chem, USA) was used to determine the plasma insulin level.
The weights of liver and previously dried caecum content samples were recorded, and the samples were homogenised in 1,5 ml of %NP40/ddH2O solution. After two repeated steps of heating (80°C–100°C for 3–5 min) and cool down (15 min at RT), all the samples were centrifuged at maximum speed. The supernatant, containing the lipids, was collected and diluted 1:10. Triacylglycerol (TAG) level in the plasma, in the liver extract and in the caecum content extract it was measured using Triglyceride Quantification Assay Kit (abcam, UK).
Leptin, glucose, insulin, and TAG levels detection were carried out in all the groups, in both experiments.
Boscaini S., Cabrera‐Rubio R., Nychyk O., Roger Speakman J., Francis Cryan J., David Cotter P, & Nilaweera K.N. (2020). Age‐ and duration‐dependent effects of whey protein on high‐fat diet‐induced changes in body weight, lipid metabolism, and gut microbiota in mice. Physiological Reports, 8(15), e14523.
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7

Plasma Collection and Hormone Quantification

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Plasma was collected from animals anesthetized with isoflurane by cardiac puncture at sacrifice. The blood was mixed with EDTA, followed by centrifugation at 10,000 g for 15 min at 4°C for plasma collection. Per the manufacturer's protocol, insulin or leptin was measured with a Mouse Insulin ELISA Kit or Mouse Leptin ELISA Kit (Crystal Chem), adiponectin with a Mouse Adiponectin ELISA Kit (Linco Research).
Fang Y., McFadden S., Darcy J., Hascup E.R., Hascup K.N, & Bartke A. (2020). Lifespan of long‐lived growth hormone receptor knockout mice was not normalized by housing at 30°C since weaning. Aging Cell, 19(5), e13123.
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8

Plasma Leptin Levels in Genetically Modified Mice

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Plasma leptin levels were measured in GNX mice of both genotypes at 8 and 20 weeks. Blood samples were collected in ethylenediaminetertracetic acid (EDTA) and a protease inhibitor cocktail (Complete, Roche Applied Science, Mannheim, Germany), and the plasma immediately harvested and stored at −20 C. For the 8 week GNX mice, leptin concentrations were measured using the Milliplex Map Mouse Metabolic Hormone Magnetic Bead Panel (Millipore, Missouri, USA, cat no. MMHMAG-44K) using Luminex technology and Exponent software in accordance with manufacturer’s instructions. The minimum detectable concentration of leptin was 19 pg/mL and the intra-assay CV% was 5%. For 20 week GNX mice, leptin concentrations were determined with a mouse leptin ELISA kit (#90030; Crystal Chem Inc.) in accordance with the manufacturer’s instructions. The minimum detectable concentration of leptin was 2 pg/mL and the intra-assay CV% was <10%.
De Bond J.A., Tolson K.P., Nasamran C., Kauffman A.S, & Smith J.T. (2016). Unaltered hypothalamic metabolic gene expression in Kiss1r KO mice despite obesity and reduced energy expenditure. Journal of neuroendocrinology, 28(10), 10.1111/jne.12430.
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9

Plasma Hormone Analysis in P16 Mice

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Trunk blood from P16 offspring was collected in EDTA-containing tubes. After centrifugation at 2,500 g for 20 min at 4 °C, plasma was aliquoted and stored at −80 °C. Insulin levels were determined using the Ultra-sensitive mouse insulin ELISA kit (#90082, Lot 21APUMI623A, Crystal Chem), leptin levels were measured using the Mouse Leptin ELISA kit (#90030, Lot 21OCML444, Crystal Chem), total ghrelin was quantified using the Rat/Mouse Ghrelin (Total) ELISA Kit (#EZRGRT-91K, Lot 3799697, Millipore), and GDF15 levels were measured using the mouse/rat GDF-15 quantikine ELISA kit (#MGD1500, R&D systems) according to the manufacturer's procedure guidelines. A SPECTROstar Nano microplate reader (BMG labtech) was used to measure absorbance.
Cantacorps L., Zhu J., Yagoub S., Coull B.M., Falck J., Chesters R.A., Ritter K., Serrano-Lope M., Tscherepentschuk K., Kasch L.S., Paterson M., Täger P., Baidoe-Ansah D., Pandey S., Igual-Gil C., Braune A, & Lippert R.N. (2023). Developmental metformin exposure does not rescue physiological impairments derived from early exposure to altered maternal metabolic state in offspring mice. Molecular Metabolism, 79, 101860.
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10

Comprehensive Metabolic Profiling in Rodents

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Blood glucose was determined at baseline and weekly thereafter using the OneTouch Ultra blood glucose meter (LifeScan). Plasma insulin levels were analyzed using an ELISA for rat insulin (Ultra Sensitive Rat Insulin ELISA; Crystal Chem). Plasma leptin levels were determined using a mouse leptin ELISA kit (Crystal Chem). Quantitative determination of mouse MCP-1 levels in plasma was performed by using an ELISA kit (R&D Systems). Plasma cholesterol was determined using a cholesterol reagent colorimetric assay kit (Roche Diagnostics). For determination of plasma HDL cholesterol levels, plasma was incubated with an HDL cholesterol precipitating reagent (Pointe Scientific, Canton, MI) followed by separation of HDL by centrifugation (2,000g, 10 min). HDL was then quantified using an enzymatic cholesterol detection kit (Roche Diagnostics).
Youn J.Y., Siu K.L., Lob H.E., Itani H., Harrison D.G, & Cai H. (2014). Role of Vascular Oxidative Stress in Obesity and Metabolic Syndrome. Diabetes, 63(7), 2344-2355.
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