Blood samples for the determination of TSH, fT4, fT3, and SHBG serum concentrations and lipid profiles were collected 8 weeks after liquid levothyroxine treatment. Hormone level measurements were performed using chemiluminescence immunoassays (CLIA) using an automated analyzer for serum concentrations of TSH, fT4, fT3, and SHBG (Advia Centaur XP Immunoassay System; Siemens, Germany). The reference ranges of hormone serum concentration were set as follows: TSH- 0.38–4.0 uIU/mL; fT4- 10,29–21,88 pmol/L; fT3- 1.71–3.71 pg/mL. The reference range for SHBG depends on age and gender. Anti-thyroid peroxidase antibodies (TPO-Ab) and antithyroglobulin antibodies (Tg-Ab) were determined in all the samples by using the CLIA method (Advia Centaur XP Immunoassay System; Siemens, Germany); antibody titers were positive above 60 IU/mL. The lipid profiles, including the levels of total cholesterol, HDL-C, LDL-C and triglycerides, were measured with a Cobas 6000 biochemistry analyzer (Roche Diagnostic, Basel, Switzerland).
Advia centaur xp immunoassay system
Manufactured by Siemens
Sourced in Germany, United States, Ireland
The ADVIA Centaur XP Immunoassay System is a fully automated, high-throughput analyzer designed for clinical laboratory testing. It is capable of performing a wide range of immunoassay tests, including those for thyroid function, infectious diseases, cardiac markers, and more. The system utilizes chemiluminescent detection technology to provide accurate and reliable results.
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Lab products found in correlation
Advia 1800, by Siemens (4 mentions)
Hlc 723g8, by Tosoh (4 mentions)
Advia 1800 chemistry system, by Siemens (3 mentions)
Advia 1800 analyzer, by Siemens (2 mentions)
Cobas e411, by Roche (2 mentions)
Immulite 2000 xpi, by Siemens (2 mentions)
Cobas integra 400 plus, by Roche (2 mentions)
Hiscl 5000, by Sysmex (2 mentions)
Cmax plus, by Molecular Devices (1 mentions)
A003 1 2, by Nanjing Jiancheng (1 mentions)
E el h0102, by Elabscience (1 mentions)
Dimension rxl max integrated chemistry system, by Siemens (1 mentions)
Advia 2120i hematology system, by Siemens (1 mentions)
Architect afp assay, by Abbott (1 mentions)
Cobas integra 400 plus analyzer, by Roche (1 mentions)
Unicel dxi 800 immunoassay analyzer, by Beckman Coulter (1 mentions)
Architect i2000sr immunoassay analyzer, by Abbott (1 mentions)
Cobas 6000 biochemistry analyzer, by Roche (1 mentions)
Advia centaur tni ultra assay, by Siemens (1 mentions)
Polymer gel for serum separation, by BD (1 mentions)
81 protocols using advia centaur xp immunoassay system
1
Thyroid Hormone and Lipid Biomarkers
2
Biochemical Assays for Metabolic Markers
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Serum fasting blood glucose, triglyceride (TG), total cholesterol (C), HDL-C, and LDL-C concentrations were determined with kits by Architect c16000 autoanalyzer (Abbott Diagnostics, USA). Vitamin B12 and folic acid concentrations were determined with Advia Centaur XP immunoassay system (Siemens, Germany) with kits by Advia Centaur XP immunoassay system.
3
Serum Biomarker Profiling in HD
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Before HD, 5 mL of blood was collected from each patient and immediately centrifuged at 3000× g for 10 min. Serum samples were stored at 4 °C within 1 h of collection. We measured serum levels of total calcium, phosphorus, alkaline phosphatase (ALP), albumin, globulin, creatinine (Cre), blood urea nitrogen, glucose, total cholesterol (TCH), and triglycerides (TG) on an autoanalyzer (Siemens Advia 1800; Siemens Healthcare GmbH, Henkestr, Germany). Intact parathyroid hormone levels (iPTH) were tested using an autoanalyzer (Siemens Advia Centaur XP Immunoassay System; Siemens Healthcare GmbH). Serum intact FGF21 levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Phoenix Pharmaceuticals, Inc. Burlingame, CA, USA). Briefly, the human FGF21 in the sample or in the standard solution can bind to the capture antibody immobilized in the wells. A standard curve of human FGF21 with known concentration can be established accordingly. The human FGF21 with unknown concentration in samples can be determined by extrapolation to this standard curve. Reactions were quantified by absorbance optical density using an automated ELISA reader (Sunrise, Tecan Co., Grödingen, Austria) at 450 nm wavelength. The intra-assay and inter-assay coefficient of variation in the measurement for FGF21 level was 3.5% and 5.2%, respectively.
4
Biochemical Markers in Hemodialysis Patients
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Approximately 5 ml of the blood sample was collected before hemodialysis from all participants. The samples were immediately centrifuged at 3000g for 10 min and stored at 4°C within one hour of collection for downstream biochemical analyses. Serum levels of total cholesterol (TCH), triglycerides, albumin, globulin, blood urea nitrogen, creatinine, glucose, total calcium, and phosphorus were measured using an autoanalyzer (Siemens Advia 1800, Siemens Healthcare, Henkestr, Germany). Serum leptin concentrations were measured using a commercially available enzyme immunoassay kit (SPI-BIO, Montigny le Bretonneux, France) [19 (link), 20 (link)]. Serum intact parathyroid hormone (iPTH) levels were measured using an autoanalyzer (Siemens Advia Centaur XP immunoassay system, Siemens Healthcare).
5
Quantification of Placental Biomarkers
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BeWo-conditioned NMM, as described above, was collected, and stored at −80°C until further use. Pregnenolone levels were determined using an ELISA kit (FR E-2700, LDN) and readout wavelength of 450 nm on a microplate reader (Cmax Plus, Molecular Devices). Progesterone analysis was performed in-house, using the ADVIA Centaur XP Immunoassay System (Siemens, Germany). MDA levels were assessed using an assay kit (A003-1-2, Nanjing Jiancheng) based on thiobarbituric acid (TBA). IL-1β, IL-4, IL-6, IL-8, IL-10, IL-13, IL-17α, IL-23, and TNF-α levels were analyzed using a suspension array (MAGPX12157001, Luminex). IL-6 levels were further verified by using an ELISA kit (E-EL-H0102, Elabscience) and readout wavelength of 450 nm on a microplate reader (Cmax Plus, Molecular Devices). All analyses were performed according to the manufacturers’ instructions.
6
Comprehensive Blood and Vitamin D Evaluation
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Complete blood count was carried out for all study subjects on ADVIA 2120i Hematology System (Siemens Healthcare GmbH, Germany). All patients were evaluated biochemically for renal function, liver function tests, electrolytes including serum calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) on the Dimension® RxL Max® Integrated Chemistry System (Siemens Healthcare GmbH, Germany). Ferritin was measured on ADVIA Centaur XP Immunoassay System (Siemens Healthcare GmbH, Germany). Serum vitamin D (25(OH)D3) levels were measured by Enzyme-linked Fluorescent Assay (ELFA) technique on VIDAS (bioMérieux Clinical Diagnostics, France). A patient was considered to have vitamin D sufficiency if 25(OH)D3 (≥20) ng/ml, insufficiency if from 10 to 19 ng/ml and vitamin D deficiency if < 10 ng/ml. 13 (link)
7
Metabolomic Profiling of Homocysteine and Related Biomarkers
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Plasma homocysteine (PHcy), urine homocysteine (UHcy) levels, plasma vitamin B12, and plasma folate levels were measured by Advia Centaur Xp Immunoassay system using dedicated kits (Siemens, Germany). Severity of PHcy elevation was assessed using criteria described by Kang et al.17 (link). Methionine and methionine/phenylalanine ratio was calculated in the dried blood spots by ABSciex 4500 triple quadrupole tandem mass spectrometer (LC–MS/MS) using Chromsystems kits, respectively (Chromsystems, Germany). Methylmalonic acid (MMA) levels in the urine were measured to rule out cases with combined homocystinuria and methylmalonic aciduria by gas chromatograph-mass spectrometer (GC–MS) using the stable isotope-labeled dilution method described by Straczek et al.18 (link) with slight modifications.
8
Detailed Protocol for Type 1 Diabetes Biomarkers
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Clinical measurements of the individuals were collected by well-trained investigators, including sex, weight, height, the age at T1D onset and some laboratory examination results, such as the FPG level, 2-h PPG level according to the OGTT test, fasting C-peptide (FCP) level and 2-h postprandial C-peptide (PCP) level. The FCP and 2-h PCP levels were detected by a chemiluminescence method (ADVIA Centaur XP Immunoassay System, Siemens, Germany) in the laboratory of the Second Xiangya Hospital, and the HbA1c level was detected by automated liquid chromatography (HLC-723G8, Tosoh, Japan). The radioligand binding assay was used in our laboratory to detect islet autoantibodies such as GADA, IA-2A and ZnT8A (21 (link)–23 (link)).
9
Serum PSA and Blood Glucose Analysis
10
Standardized AFP Serum Analysis
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Serum samples throughout the region are collected in a unified blood collection system and analysed in a central laboratory using ADVIA Centaur XP Immunoassay System (Siemens) or Architect AFP assay (Abbott Laboratories) which were validated for comparability. Results are reported as kU/L (Conversion factor 0.83kU/L = 1ug/L).
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