In brief, spleens were prepared for flow cytometry by generating single cell suspensions. RBCs were lysed by Tris Ammonium Chloride. Single cell suspensions of bone marrow cells were prepared by flushing the marrow followed by RBC lysis. Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93- Biolegend) and stained with combinations of the following antibodies: anti-B220-PacBlue (RA3–6B2), GL7-FITC, anti-CD95-PeCy7 (Jo2), anti-CD4-Alexa Flour 700 (RM4–5), anti-CXCR5-biotin (2G8), anti-PD-1-PE (29F.1A12), anti-CD11c-BV421 (N418), anti-NK1.1-biotin (PK136), anti-Ly6G-BV711 (1A8), CD86-PeCy5 (GL-1), anti-CD44-BV605/APC (IM7), anti-CD62L-PeCy7 (MEL-14), anti-CD80-PE (16–10A1), anti-CD19-BV605 (6D5), anti-CD267-PE (8F10), anti-CD138-PeCy7 (281–2), and SA-PeCy5 from Biolegend; anti-CD43-FITC (S7), anti-Ly6C-FITC (AL-21), anti-CD11b-AF700 (M1/70), anti-CD90.2-biotin (53–2.1), anti-CD19-biotin (1D3), anti-CD40-FITC (HM40–3), anti-IgD-APC (11–26c.28), and SA-V500 from BD Biosciences. Viability staining was performed by incubating samples with Fixable Viability Dye (e780) from eBioscience. Data were acquired on an LSRII flow cytometer (BD Biosciences) and data were analyzed using FlowJo software (Tree Star).
Anti cd44 bv605 apc
Manufactured by BioLegend
Anti-CD44-BV605/APC is a fluorochrome-conjugated antibody that binds to the CD44 cell surface antigen. CD44 is a transmembrane glycoprotein involved in cell-cell interactions, cell adhesion, and migration. This product is intended for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsrii flow cytometer, by BD (2 mentions)
Anti pd 1 pe, by BioLegend (2 mentions)
Anti cd95 pecy7, by BioLegend (2 mentions)
Anti cd11c bv421, by BioLegend (2 mentions)
Anti cd80 pe, by BioLegend (2 mentions)
Trustain fcx, by BioLegend (2 mentions)
Fixable viability dye, by Thermo Fisher Scientific (2 mentions)
Gl7 fitc, by BioLegend (2 mentions)
Cd86 pecy5, by BioLegend (2 mentions)
Anti cd62l pecy7, by BioLegend (2 mentions)
Anti cxcr5 biotin, by BioLegend (2 mentions)
Anti nk1.1 biotin, by BioLegend (2 mentions)
Anti ly6g bv711, by BioLegend (2 mentions)
Anti cd138 pecy7, by BioLegend (2 mentions)
Sa pecy5, by BioLegend (2 mentions)
Sa v500, by BD (2 mentions)
Anti cd19 bv605, by BioLegend (1 mentions)
Anti cd11b af700, by BD (1 mentions)
Anti cd40 fitc, by BD (1 mentions)
2 protocols using anti cd44 bv605 apc
1
Spleen and Bone Marrow Flow Cytometry
2
Multiparameter Flow Cytometry of Spleen and Bone Marrow
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In brief, spleens were prepared for flow cytometry by generating single cell suspensions. RBCs were lysed by Tris Ammonium Chloride. Single cell suspensions of bone marrow cells were prepared by flushing the marrow followed by RBC lysis. Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93-Biolegend) and stained with combinations of the following antibodies: anti-B220-PacBlue (RA3-6B2), GL7-FITC, anti-CD95-PeCy7 (Jo2), anti-CD4-Alexa Flour 700 (RM4-5), anti-CXCR5-biotin (2G8), anti-PD-1-PE (29F.1A12), anti-CD11c-BV421 (N418), anti-NK1.1biotin (PK136), anti-Ly6G-BV711 (1A8), CD86-PeCy5 (GL-1), anti-CD44-BV605/APC (IM7), anti-CD62L-PeCy7 (MEL-14), anti-CD80-PE (16-10A1), anti-CD19-BV605 (6D5), anti-CD267-PE (8F10), anti-CD138-PeCy7 (281-2), and SA-PeCy5 from Biolegend; anti-CD43-FITC (S7), anti-Ly6C-FITC (AL-21), anti-CD11b-AF700 (M1/70), anti-CD90.2-biotin (53-2.1), anti-CD19-biotin (1D3), anti-CD40-FITC (HM40-3), anti-IgD-APC (11-26c.28) , and SA-V500 from BD Biosciences. Viability staining was performed by incubating samples with Fixable Viability Dye (e780) from eBioscience. Data were acquired on an LSRII flow cytometer (BD Biosciences) and data were analyzed using FlowJo software (Tree Star).
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