Gcms qp2010 ultra instrument

Soluble sugars were extracted from the roots of Chinese cabbage at weeks 2, 3, 4, and 5 after P. brassicae inoculation according to the previous method [45 (link)]. The GC-MS QP2010 ultra instrument (Shimadzu, Kyoto, Japan) was used for the gas quality analyses. The detailed setting information is as follows: the inlet temperature was 300 °C, the split ratio was 10:1, the carrier gas was high-purity helium, and the flow rate was 1 mL/min. The heating program was as follows: 120 °C for 3 min, 5 °C/min to 210 °C for 5 min, and 15 °C/min to 300 °C for 10 min. The ion source temperature was 200 °C, and the interface temperature was 280 °C. The solvent removal time was 3 min, and the scanning m/z was 45–500. Soluble sugars were extracted from three biological replicates at each time point. Gas quality analyses were repeated three times for each treatment.

A GC–MSQP2010 Ultra instrument (Shimadzu Technologies Inc., Japan) with an EI ion source, quadrupole mass analyser, and AOC-20i autosampler was used. The chromatographic separation of metabolites was performed on an HP-5 ms capillary column (30 m × 0.25 mm × 0.25 μm) (Agilent J&W Scientific). A Termovap Sample Concentrator (Automatic Science Instrument Co., Ltd, China), an Alpha1-2Ldplus Freeze dryer (CHRIST Co., Germany), an MSC-100 constant temperature homogenizer (Aosheng Instrument Co. Ltd., Hangzhou, China), a TGL-16B high-speed centrifuge (Anting Instrument Co. Ltd., Shanghai, China), and a KQ2200E ultrasonic cleaner (40 kHz, 100 W, Kunshan Ultrasonic Instrument Co. Ltd., Kunshan, China) were employed in this study.

Measurements were performed on a Shimadzu GCMS-QP2010 Ultra instrument (Shimadzu, Kyoto, JP) using a SH-Rxi-5Sil MS column (30 m x 0.25 mm, 0.25 µm film, Shimadzu, Kyoto, JP) or OPTIMA 5MS Accent column (25 m x 0.20 mm, 0.20 µm film, Macherey-Nagel, Düren, DE) and helium as carrier gas. 1 µl of each sample was injected splitless with an injection temperature of 280 °C. The split/splitless uniliner inlets (3.5 mm, 5.0 × 95 mm for Shimadzu GCs, deactivated wool) from Restek (Bad Homburg, DE) were utilised and regenerated if needed by CS-Chromatography (Langerwehe, DE). The temperature program was adjusted, as shown in Supplementary Table 7. The interface temperature was set to 290 °C. Ionisation was obtained by electron impact with a voltage of 70 V, and the temperature of the ion source was 250 °C. The MS is equipped with dual-stage turbomolecular pumps and a quadrupole enabling a selected ion monitoring acquisition mode (SIM mode). Calibration and quantification were implemented in SIM mode with the corresponding m/z traces, as shown in Supplementary Table 7. The detector voltage of the secondary electron multiplier was adjusted in relation to the tuning results with perfluorotributylamine. The GC–MS parameter was controlled with GCMS Real Time Analysis, and for data evaluation, GCMS Postrun Analysis (GCMSsolution Version 4.45, Shimadzu, Kyoto, JP) was used.

The asymmetric epoxidation of alkenes over the Ti-Beta-TEAOH and normal Ti-Beta was carried out under the following conditions: 0.2 g catalyst, 10 mL acetonitrile, substrate/H₂O₂ (35% in aqueous solution) = 1, 70 °C, 2 h. The products were identified by a Shimadzu GCMS-QP 2010 Ultra instrument equipped with a Rtx-5MS column and/or comparing with standard (2S,3S) and (2R,3R)-epoxide. Enantioselectivities were determined by chiral GC (Shimadzu, GC-2010 Plus) equipped with a CP-Chirasil-Dex CB column.

As some isomeric sugar species cannot be sufficiently resolved with the untargeted GC×GC-MS approach [26 (link)] but may play an important role in human metabolism, a complementary targeted GC-MS sugar profiling method was developed for urine samples using a Shimadzu GCMS QP2010 Ultra instrument. Using a Scan-/ selected ion monitoring (SIM)-approach, a higher selectivity and a sufficient sensitivity were achieved. Furthermore, by monitoring common and well-known sugar fragments, e.g., m/z 217, 307, and 361 as well as fragments specific for sugar-related compounds like m/z 292, 333, or 318, known as well as unknown sugar species could be detected. Additionally, some abundant non-sugar compounds always present in urine, such as creatinine, were also captured. This enabled verification of the results of the GC×GC-MS approach. Overall, 66 metabolites, consisting of 40 known sugar species, 15 unknown sugar species, and 11 non-sugar-compounds, were detected with this method. For analytical details see section A and C of the S1 File.

GC-MS measurements were performed on a Shimadzu GCMS-QP2010 Ultra instrument (Kyoto, Japan). Splitless mode (valve opened at 1 min) was used to inject the final extracts into the gas-chromatograph interfaced with a mass selective detector. The chromatographic column was a Sapiens-5MS+ capillary column (30 m × 0.25 mm internal diameter × 0.25 μm film thickness) from Teknokroma (Barcelona, Spain). with helium as a carrier gas at a constant velocity (50 cm/s). The oven temperature conditions were as follows: started at 100 °C, maintained at this temperature for 2 min, elevated at 15 °C min⁻¹ to 115 °C, increased at 80 °C min⁻¹ until 300 °C and maintained for 6 min at 300 °C. The total run time was 11 min. The ion source and transfer line temperatures were set to 250 °C and 280 °C, respectively. Following a 2.5 min solvent delay, the mass detector was operated in synchronous selected ion monitoring (SIM) mode (m/z 117, 118, 217, 218, 191, 195, 231) using a dwell time of 150 ms and scan mode ranging from m/z 65 to m/z 280 m/z using a dwell time of 3 ms. Identification of the analytes in the sample extracts was achieved via GC retention time and comparison with reference standards. One μL was injected into the chromatographic system and four pre- and post-injection washes (in cyclohexane) were performed between injections.