The reagents we used included the following: PP-IX, Zn-PP, TMPD, ethyl sorbate, sodium azide (NaN3), mannitol, glucose oxidase, and superoxide dismutase were purchased from Sigma-Aldrich. Catalase was from Calbiochem (Burlington, MA). Hemin, Uro, and Copro were obtained from Frontier Scientific (Logan, UT). POBN was from Cayman Chemicals (Ann Arbor, MI). The following stock solutions were prepared for subsequent use: PP-IX (1.78 mmol/L in dimethylacetamide [DMA]); Hemin (1.53 mmol/L in 100 mmol/L NaOH); Zn-PP (0.5 mmol/L in DMA); Uro (5 mmol/L in 100 mmol/L NaOH); Copro (5 mmol/L in 100 mmol/L NaOH); NaN3 (100 mmol/L in 400 mmol/L phosphate buffer, pH 7.4); mannitol (100 mmol/L in 400 mmol/L phosphate buffer, pH 7.4); TMPD (121.7 mmol/L in DMA); and trolox (100 mmol/L in DMA). A detailed list of the reagents used is included in Appendix I .
Sodium azide nan3
Manufactured by Merck Group
Sourced in United States, Germany, China, Italy, Poland
Sodium azide (NaN3) is an inorganic compound that is commonly used as a laboratory reagent. It is a white crystalline solid that is soluble in water and other polar solvents. The core function of sodium azide is as a chemical precursor and preservative.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (7 mentions)
Methanol, by Merck Group (6 mentions)
Rose bengal rb, by Merck Group (3 mentions)
Furfuryl alcohol ffa, by Merck Group (3 mentions)
Chloroform, by Thermo Fisher Scientific (3 mentions)
Tween 20, by Merck Group (3 mentions)
Ascorbic acid, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
K2hpo4, by Merck Group (3 mentions)
Nystatin, by Merck Group (2 mentions)
Potassium carbonate k2co3, by Merck Group (2 mentions)
Triethylamine, by Merck Group (2 mentions)
Methyl methanesulfonate mms, by Merck Group (2 mentions)
Carbonyl cyanide m chlorophenyl hydrazone, by Merck Group (2 mentions)
Antimycin a, by Merck Group (2 mentions)
Rotenone, by Merck Group (2 mentions)
5 5 dimethyl 1 pyrroline n oxide dmpo, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Tsp d4, by Merck Group (2 mentions)
87 protocols using sodium azide nan3
1
Porphyrin Compound Activity Assay
2
Synthesis of 2-thiouracil using NaN3
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2-Thiouracil (2-TU) (≥99% purity) and sodium azide (NaN3) (≥99.5% purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Nitrous oxide (N2O) > 98% was from Messer (Warsaw, Poland).
3
Biochemical Assays for Antioxidant and Enzymatic Analysis
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Reduced glutathione (GSH), adenosine triphosphate (ATP), acetylthiocholine iodide, 6,7-Dimethyl-5,6,7,8-tetrahydropterine (DMTHP), 5’,5’- dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), xanthine (2,6-Dihydroxypurine), adrenaline, ammonium molybdate, oxidized nicotinamide adenine dinucleotide (NAD), reduced nicotinamide adenine dinucleotide (NADH), tyrosine, tetramethylbenzidine, L-glutamate, sodium azide (NaN3) and amino naphtol sulphonic acid (ANSA) were products of Sigma-Aldrich (St. Louis, MO, USA). Other chemicals and reagents used were of analytical grade.
4
Detailed Chromatographic Protocol for Compound Purification
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All extracting and column chromatography (CC) reagents were reagent grade. Merck silica gel (100-200 mesh ASTM) was used for CC chromatography. TLC was performed on precoated silica gel aluminum sheets (silica gel 60 F254, 0.20 mm, Merck). Fractions were monitored by UV (254 nm), and by a ceric sulfate reagent followed by heating. Peptone bacto, yeast extract, agar bacto, Mueller Hinton (MH) agar, MH broth, trypticase soy agar, Luria broth (LB), trypticase soy broth, sabouraud dextrose agar, sabouraud dextrose broth, minimal glucose agar (MGA) and dextrose were purchased from Difco (Sparks, MD). Methanol (HPLC grade), methanol (spectrophotometric grade), camptothecin (CPT), etoposide (ETP), dimethyl sulfoxide (DMSO-Hybri-Max), adenine hemisulfate salt, chloranphenicol, nystatin, ascorbic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), benzo [a] pyrene (B[a]P), and sodium azide (NaN3) were obtained from Sigma Chemical (St. Louis, MO).
5
NMR Metabolomics Sample Preparation
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Analytical grade methanol, K2HPO4·3H2O, NaH2PO4·2H2O and sodium azide (NaN3) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium 3-trimethylsilyl [2,2,3,3−2H4]-propionate (TSP) and deuterium oxide (D2O, 99.9% D) were purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). The phosphate buffer for NMR analysis was prepared by dissolving K2HPO4 and NaH2PO4 in water (0.15 M, pH 7.44, K2HPO4/NaH2PO4 = 4:1) containing 0.001% TSP, 0.1% NaN3, and 50% D2O [54 (link)].
6
Chemical Standards for Analysis
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Aflatoxin B1 (AFB1) and sodium azide (NaN3) were obtained from Sigma-Aldrich (St. Louis, USA). 2-Amino-3,4 dimethylimidazo[4,5-f]quinolone (MeIQ), 2-aminoanthracene (2-AA) and 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) were purchased from Wako Pure Chemicals (Osaka, Japan). Collagenase type IV and 4′-6-diamidino-2-phenylindole (DAPI) were obtained from Gibco/ Invitrogen Corp. (Carlsbad, USA). The phenolic acid, flavonoid and anthocyanin standards for chemical analysis were high performance liquid chromatography grade. All other chemicals were at least analytical grade.
7
Comprehensive Chemical Standard Procurement
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Standard mutagens, including AFB1, sodium azide (NaN3), and 2-aminoflurene (2-AF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-aminoanthracene (2-AA), 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2), 2-amino-3,4-dimethylimidazo[4,5-f] quinoline (MeIQ), and benzo(a)pyrene (B(a)P) were obtained from Wako Pure Chemicals Industries Ltd. (Osaka, Japan). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ascorbic acid were purchased from Merck (Darmstadt, Germany). The phenolic acids, flavonoids, anthocyanins, sodium phytate, γ-oryzanol, and tocols standards for chemicals analysis were high performance liquid chromatography (HPLC) grade. All other chemicals were analytical grade.
8
Genotoxicity Assay Positive Controls
9
Synthesis and Evaluation of Acridine-based Anticancer Agents
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2,7-Dimethylacridine-3,6-diamine
(acridine yellow, AY, L-1), 3,8-diamino-5-ethyl-6-phenyl-phenanthridin-5-ium
bromide (ethidium bromide, EB, L-2), pyrrole, trifluoroacetic acid,
2-/4-hydroxybenzaldehyde, 1,2-dibromoethane, sodium azide (NaN3), triethylamine, borontrifluoride diethyletherate (BF3·Et2O), 2,3-dichloro-5,6-dicyano-1,4-benzoquinone,
potassium carbonate (K2CO3), propargyl bromide,
2-acetylpyridine, sodium hydroxide (NaOH), and ammonia (NH3, liq.) were purchased from Sigma-Aldrich Chemical Co., USA and used
as received. Solvents were purchased from Merck, and S D Fine-Chem
Ltd., Mumbai, India. The solvents were dried and distilled, following
the standard procedures prior to their use.67 Trypsin–ethylenediaminetetraacetic acid solution and MTT
were purchased from HiMedia, India, whereas DMSO from GeNie, Merck,
India. Dulbecco’s modified Eagle’s medium (DMEM), fetal
bovine serum, and antibiotic solution (10 000 units/mL penicillin
and 10.0 mg/mL streptomycin) were purchased from Cellclone, Genetix
Biotech Asia Pvt. Ltd. MDA-MB-231 a breast cancer cell line was procured
from National Centre for Cell Science (NCCS), Pune.
(acridine yellow, AY, L-1), 3,8-diamino-5-ethyl-6-phenyl-phenanthridin-5-ium
bromide (ethidium bromide, EB, L-2), pyrrole, trifluoroacetic acid,
2-/4-hydroxybenzaldehyde, 1,2-dibromoethane, sodium azide (NaN3), triethylamine, borontrifluoride diethyletherate (BF3·Et2O), 2,3-dichloro-5,6-dicyano-1,4-benzoquinone,
potassium carbonate (K2CO3), propargyl bromide,
2-acetylpyridine, sodium hydroxide (NaOH), and ammonia (NH3, liq.) were purchased from Sigma-Aldrich Chemical Co., USA and used
as received. Solvents were purchased from Merck, and S D Fine-Chem
Ltd., Mumbai, India. The solvents were dried and distilled, following
the standard procedures prior to their use.67 Trypsin–ethylenediaminetetraacetic acid solution and MTT
were purchased from HiMedia, India, whereas DMSO from GeNie, Merck,
India. Dulbecco’s modified Eagle’s medium (DMEM), fetal
bovine serum, and antibiotic solution (10 000 units/mL penicillin
and 10.0 mg/mL streptomycin) were purchased from Cellclone, Genetix
Biotech Asia Pvt. Ltd. MDA-MB-231 a breast cancer cell line was procured
from National Centre for Cell Science (NCCS), Pune.
10
Genotoxicity Evaluation of Chemicals
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Sodium azide (NaN3) (CAS No. 26628-22-8), methylmethanesulfonate (MMS) (CAS No. 66-27-3), 2-aminoanthracene (2-AA) (CAS No. 613-13-8), cyclophosphamide (CPA) (CAS No. 50-18-0), and colchicine (CAS No. 64-86-8) were obtained from Sigma-Aldrich. 4-nitro-o-phenylene-diamine (4-NOPD) (CAS No. 99-56-9) was from Fluka. These chemicals were used as positive controls in the genotoxicity studies.
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