Beta-cyclodextrin (βCD), gallic acid (GA), catechin (CAT), and epigallocatechin gallate (EGCG) were purchased from Merck SA (Pty), Sandton, South Africa (SA). For digestion, salivary amylase, pepsin from porcine gastric mucosa, pancreatin from porcine pancreas, porcine bile extract, Pefabloc® and associated salts, and the reagents for the antioxidant studies were purchased from the same company. For electrospray ionization tandem mass spectrometry and electron microscopy, reagents were analytical grade and were obtained from Merck SA (Pty), SA. Cell culture media was also obtained from Merck SA (Pty), SA and fetal calf serum (FCS) were obtained from Capricorn Scientific, GmbH, Germany, and the Caco-2 cell line was obtained from CELLONEX, Separations, SA.
Cell culture media
Manufactured by Merck Group
Sourced in United States, Germany, Ireland, United Kingdom, Italy, Hungary, Spain, France
Cell culture media is a nutrient-rich liquid or gel-like substance that provides the necessary components for the growth and maintenance of cells in a laboratory setting. It contains a variety of vitamins, amino acids, carbohydrates, and other essential nutrients required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Fetal bovine serum (fbs), by Merck Group (4 mentions)
Tissue culture dishes, by Euroclone (3 mentions)
1 2 3h cortisone, by American Radiolabeled Chemicals (3 mentions)
Trypsin, by Merck Group (3 mentions)
Hybridoma cell lines, by American Type Culture Collection (2 mentions)
Calcein, by Merck Group (2 mentions)
Formic acid, by Biosolve (2 mentions)
Cell culture lysis reagent, by Promega (2 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions)
Alexa 647 succinimidyl ester a647, by Thermo Fisher Scientific (2 mentions)
3h imipramine, by PerkinElmer (2 mentions)
Trans ferulic acid, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Cell culture material, by Greiner (2 mentions)
1 2 6 7 3h cortisol, by PerkinElmer (2 mentions)
2 4 6 7 3h estrone, by PerkinElmer (2 mentions)
2 4 6 7 3h estradiol, by PerkinElmer (2 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (2 mentions)
83 protocols using cell culture media
1
Bioactive Compounds Digestion and Antioxidant Assay
2
Comprehensive Reagent Protocols
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General laboratory reagents were from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) and Molar Chemicals Kft (Budapest, Hungary). Cell culture media and associated reagents were from Sigma-Aldrich (Merck KGaA), specific reagents for polymerase chain reaction (PCR) were from Thermo Fisher Scientific, Inc., (Waltham, MA, USA).
3
Cytotoxicity Assays for MC^(cd34) and DC^(cd34) Cells
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MCcd34 and DCcd34 cultures seeded at an initial density of 5 × 104 cells/ml in 48 well plates were treated with paraquat (Merck) or busulfan (Merck) for 24 h. Select treatments were also carried out with house dust mite soluble extracts (Stallergenes Greer, US). Cellular resazurin reduction and lactate dehydrogenase (LDH) into the media were used as measures of viability. cell culture media LDH content was indicative of necrotic cellular leakage and determined after centrifugation using a commercial kit (Merck; Cat# 4744926001) according to manufacturer's instructions. Resazurin reduction as a measure cellular and mitochondrial reductase activity was carried out as previously described (Meldrum et al., 2016 (link)). Briefly, cells were washed in PBS and incubated 44 μM resazurin in cell culture media (Cat# R7017, Merck) for 1 h at 37C. Fluorescence was then detected at 540 nm excitation and 590 nm emission. Statistical significance compared to control values was carried out using one-way ANOVA and Fisher's LSD test in GraphPad Prism software Version 8.3.0 and results are expressed as mean ± standard error of the mean (SEM) unless otherwise stated.
4
Cell Culture Reagents and Materials
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Chemicals, cell culture media, and supplements were obtained from Sigma Aldrich, Milan, Italy, unless otherwise specified, and tissue culture dishes were purchased from Euroclone, Milan, Italy.
5
Culturing P815-HTR Mouse Mastocytoma Cells
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P815-HTR mouse mastocytoma line was obtained from ATCC. Cell culture media and chemicals were purchased from Sigma-Aldrich, unless otherwise specified. The P815-HTR cells was grown in 100-mm culture dishes at 37 °C under a humidified atmosphere of 5% CO2 and 95% air. The cells were maintained under culture conditions in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 20 µg/ml gentamicin and with or without added 4 mM L-glutamine (GLN).
6
Labeling Anti-Pgp Monoclonal Antibodies
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Cell culture media, supplements and chemicals were from Sigma-Aldrich (Budapest, Hungary). Fluorescent dyes including calcein acetoxymethyl ester (calcein-AM), BODIPY FL vinblastine (vinblastine-bodipy; VBL-BPY) and Alexa 647 succinimidyl ester were purchased from Life Technologies, Inc. (Carlsbad, CA, USA). The UIC2 and 15D3 anti-Pgp mAbs were prepared from hybridoma supernatants using affinity chromatography and were >97% pure by SDS/PAGE. Hybridoma cell lines were obtained from the American Type Culture Collections (Manassas, VA, USA). The UIC2 and 15D3 antibodies were labeled with Alexa 647 succinimidyl ester (A647) and separated from the unconjugated dye by gel filtration on a Sephadex G-50 column. The dye-to-protein labeling ratio was around 3 for each antibody preparation.
7
Osteogenesis-Inducing Cell Culture
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Cell culture media, supplements and calcein was purchased from Sigma–Aldrich (St. Louis, MO). Antibodies for western blot analysis and immunohistochemistry were obtained from cell signaling technology (Danvers, MA, USA) and Santa Cruz Biotechnology (CA, USA). Human PTH (1–34) was purchased from Calbiochem (Darmstadt, Germany).
8
Maintenance of SKOV-3 and U87 Cell Lines
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SKOV‐3 (human ovarian carcinoma) cell line were maintained as adherent cells at 37 °C and a humidified 5% CO2 atmosphere in RPMI‐1640 medium supplemented with 10% FBS, 1% l ‐glutamine and 1% penicillin/streptomycin solution. The U87 (human glioblastoma) cell line was maintained, at the same condition, in DMEM medium supplemented with 10% FBS, 1% l ‐glutamine, 1% sodium pyruvate and 1% penicillin/streptomycin solution. Cell lines, cell culture media and supplements were purchased from Sigma (Milan, Italy); plastics were purchased by Euroclone SpA (Milan, Italy).
9
Synthesis and Characterization of Dox/ONBs
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All lipids, namely, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG-2000-Amine), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (DSPE-PEG-2000-Biotin) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). An OxiSelect intracellular ROS assay kit for the fluorometric detection of ROS was purchased from Cell Biolabs Inc. (San Diego, CA, USA). A 99.995% pure oxygen cylinder (Daesung gas, Siheung-si, Gyeonggi-do, South, Korea) was used for oxygen supply during the synthesis of Dox/ONBs. Doxorubicin-HCL, chloroform, cell culture media, and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). DoxovesTM (Liposomal Doxorubicin-HCL) was purchased from FormuMax Scientific (Sunnyvale, CA, USA).
10
Quantification of Oxysterol Metabolites
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7k27OHC, 7β27OHC, 7α27OHC, and 7β27OHC-d6 were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL), [1,2-3H]cortisone, [1,2,6,7-3H]cortisol, and [1,2,6,7-3H] corticosterone from American Radiolabeled Chemicals (St. Louis, MO), and all other chemicals from Sigma-Aldrich (Buchs, Switzerland) of the highest grade available. Cell culture media were purchased from Sigma-Aldrich and Invitrogen (Carlsbad, CA) and FBS approved for use with the Tet-on system from Clontech (Mountain View, CA). UHPLC-grade purity methanol, acetonitrile, and formic acid were from Biosolve (Dieuze, France). 5H-1,2,4-triazolo(4,3-a)azepine,6,7,8,9-tetrahydro-3-tricyclo(3-3-1-13-7)dec-1-yl [T0504; also known as Merck-544 (25 (link))] was purchased from Enamine (Kiev, Ukraine).
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