Type 7600 020 automated analyzer

Blood samples were collected after an overnight fast and 2 h after a 75 g oral glucose tolerance test (OGTT). Plasma glucose concentrations were measured using the glucose oxidase-peroxidase method with commercial kits (Shanghai Biological Products Institution, Shanghai, China). Glycated hemoglobin values were determined by high-performance liquid chromatography performed on a Bio-Rad Variant II hemoglobin testing system (Bio-Rad Laboratories, Hercules, CA, USA). Serum lipid profiles, including total cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured with a type 7600-020 Automated analyzer (Hitachi, Tokyo, Japan). The serum levels of insulin and proinsulin were measured in duplicate at 0, 2, 4, and 6 min after an intravenous injection of 50 mL arginine solution at the concentration of 10%, using radioimmunoassay (Linco Research, St Charles, MO, USA).

Blood and urine samples were obtained after an overnight fast to measure the analytes described below. Serum lipid profiles, including total cholesterol (TC), triglyceride (TG), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), blood urea, blood creatinine, blood uric acid, blood urea nitrogen, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transSupeptidase (γ-GT), and urine creatinine were determined with a type 7600-020 automated analyzer (Hitachi, Tokyo, Japan).