Thierry zepto

Manufactured by Diener Electronic

The Thierry Zepto is a compact and versatile lab equipment used for precise measurements and analysis. It features advanced technology to ensure reliable and accurate results. The core function of the Thierry Zepto is to provide precise measurements and data analysis for scientific applications.

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Lab products found in correlation

Biopsy punch, by Ted Pella (1 mentions)

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Zepto

2 protocols using thierry zepto

Microfluidic Channels for Cell Culture

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Microfluidic channels were designed using SolidWorks and were subsequently patterned on silicon wafers (University Wafer Corp.) using photolithography. The microfluidic channels were then prepared using soft lithography of polydimethylsiloxane (PDMS, Dow Corning). Inlet and outlet holes were made with a 1.5 mm wide biopsy punch (Ted Pella). Each device had two independent parallel channels and the PDMS block containing the features was bonded to a PDMS coated glass slide (75 × 25 mm) using a 100 Watts plasma cleaner (Thierry Zepto, Diener Electronic). An open slip-tip syringe was connected to the channels through a curved dispensing tip (Qosina), which acted as a liquid reservoir for growth media, blood etc. wherever required. The outlet was connected to a syringe pump (Harvard Apparatus, PHD Ultra) using a 20” tubing (Qosina).

Mathur T., Singh K.A., Pandian N.K., Tsai S.H., Hein T.W., Gaharwar A.K., Flanagan J.M, & Jain A. (2019). Organ-on-Chips made of Blood: Endothelial Progenitor Cells from Blood reconstitute Vascular Thromboinflammation in Vessel-Chips. Lab on a chip, 19(15), 2500-2511.

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Microfluidic Venous Valve Patterning

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Masks needed to pattern these designs using photolithography were obtained from CAD-Art services (Bandon, Orlando). The designs were subsequently patterned on Si (100) wafers (University Wafer Corp) at Aggie-Fab nanofabrication facility of Texas A&M University. The microfluidic channels were then prepared using soft lithography of polydimethylsiloxane (PDMS, Dow Corning). Each device had three independent parallel channels with each of them having three 25% open, 50% open or 75% open venous valves. Inlet and outlet holes for the devices were made with a 1.5 mm wide biopsy punch (Ted Pella). PDMS block containing the features was bonded to either a PDMS coated glass slide (75 × 25 mm) or a block of PDMS or coverslips using a 100 Watt plasma cleaner (Thierry Zepto, Diener Electronic). Devices made on glass slides, PDMS block and coverslip are used for blood perfusion experiment, staining for proteins of interest at cusps and confocal imaging of lumen respectively. An open slip-tip syringe was connected to the devices through a curved dispensing tip (Qosina) which acted as a reservoir. The outlet was connected to a syringe pump (Harvard Apparatus, PHD Ultra) using a 20” tubing (Qosina).

Pandian N.K., Walther B.K., Suresh R., Cooke J.P, & Jain A. (2020). Microengineered Human Vein-Chip Recreates Venous Valve Architecture and its Contribution to Thrombosis. Small (Weinheim an der Bergstrasse, Germany), 16(49), e2003401.

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