1 1 diphenyl 2 picrylhydrazyl

Manufactured by Yuanye Bio-Technology

Sourced in China

1,1-diphenyl-2-picrylhydrazyl is a stable free radical compound commonly used as a reagent in biochemical and analytical applications. Its core function is to serve as an indicator for antioxidant activity and free radical scavenging properties.

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Lab products found in correlation

9 protocols using 1 1 diphenyl 2 picrylhydrazyl

Phytochemical Characterization and Antioxidant Capacity

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Vanillin, hydrochloric acid, glacial acetic acid, ferric chloride, sulfuric acid, anhydrous sodium carbonate, sodium nitrite, sodium acetate, and other reagents were purchased from Bodi Chemical (Tianjin, China) and were analytically pure. HPLC-grade methanol and acetonitrile were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The Folin–Ciocalteu regent, Trolox, 2, 4, 6-tris(2-pyridyl)-s-triazine (TPTZ), 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, rutin, catechin, and 2, 2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) were acquired from Yuanye Bio-Technology (Shanghai, China).

Bai Z., Tang J., Li Y., Li Z., Gu S., Deng L, & Zhang Y. (2024). Integrated Metabolomics Approach Reveals the Dynamic Variations of Metabolites and Bioactivities in Paeonia ostii ‘Feng Dan’ Leaves during Development. International Journal of Molecular Sciences, 25(2), 1059.

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Comprehensive Quantification of Phytochemicals

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The main chemicals used in this experiment included γ-aminobutyric acid (AR, Macklin, Shanghai, China), anthrone (AR, Kefeng, Shanghai, China), ethyl acetate (AR, Xilong, Guangdong, China), Sodium hydroxide (AR, Keshi, Chengdu, China), sucrose (standard products, Yuanye, Shanghai, China), glucose (standard products, Yuanye, Shanghai, China), fructose (standard products, Yuanye, Shanghai, China), oxalic acid (standard products, Yuanye, Shanghai, China), succinic acid (standard products, Yuanye, Shanghai, China), quinic acid (standard products, Yuanye, Shanghai, China), shikimic acid (standard products, Yuanye, Shanghai, China), citric acid (standard products, Yuanye, Shanghai, China), malic acid (standard products, Yuanye, Shanghai, China), acetonitrile (GR, Knowles, Chengdu, China), quercetin (AR, Solarbio, Beijing, China), gallic acid (AR, Keshi, Chengdu, China), Sodium nitrite (AR, Keshi, Chengdu, China), Nitro blue tetrazolium (BR, Yuanye, Shanghai, China), 1,1-Diphenyl-2-picryl-hydrazyl (BR, Yuanye, Shanghai, China), 2,3,5-triphenyltetrazolium chloride (AR, Solarbio, Beijing, China).

Zhang Y., Lin B., Tang G., Chen Y., Deng M., Lin Y., Li M., He W., Wang Y., Zhang Y., Luo Y., Chen Q., Wang X, & Tang H. (2024). Application of γ-aminobutyric acid improves the postharvest marketability of strawberry by maintaining fruit quality and enhancing antioxidant system. Food Chemistry: X, 21, 101252.

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HPLC-MS Analysis of Platycodonis radix

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LC/MS-grade water, acetonitrile and methanol were purchased from Merck KGaA (Darmstadt, Germany). LC/MS-grade formic acid was purchased from Thermo Fisher Scientific (Shanghai, China). Reference standards (i) deapioplatycodin D3, deapio-platycodin D, platycodin D2, polygalacin D, 1,1-diphenyl-2-picrylhydrazyl (DPPH) were provided by Yuanye bio-technology Co. Ltd (Shanghai, China). (ii) Lobetyolinin was purchased from A Chemtek Inc. (MA, USA). (iii) Nystose, lobetyolin and chlorogenic acid were got from national institutes for food and drug control (Beijing, China). Platycodonis radix used in this study, 12 samples from each origin, were collected form the local agricultural planting base of Taihe county in AH and Chifeng city in NM. All samples were authenticated based on the botany traits as recorded in Flora of China by Professor Yajun Cui (Shanghai University of Traditional Chinese Medicine) and the voucher specimen was deposited at the author's laboratory. Detailed information of these samples is provided in Table S1.†

Huang W., Lan L., Zhou H., Yuan J., Shui m.i.a.o., Mao X., Hu Q, & Ji S. (2022). Comprehensive profiling of Platycodonis radix in different growing regions using liquid chromatography coupled with mass spectrometry: from metabolome and lipidome aspects. RSC Advances, 12(7), 3897-3908.

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Antioxidant Properties of Cherry Tomatoes

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Cherry tomatoes (Millennium variety) were purchased from Huilong Farmer’s Market in Jinzhong City. Gelatin (Bloom strength ≥ 250 g, from bovine bones), glycidyltrimethylammonium chloride (GTA, ≥95%), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). Rosmarinic acid (RosA, 97%) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd (Shanghai, China). 2,4,6-trinitrobenzenesulfonic acid was acquired from Sigma-Aldrich (St. Louis, MO, USA). All other chemical reagents were of analytical grade.

Qiao J., Wang L., Wang L., Li Z., Huai Y., Zhang S, & Yu Y. (2024). Development and Characterization of Modified Gelatin-Based Cling Films with Antimicrobial and Antioxidant Activities and Their Application in the Preservation of Cherry Tomatoes. Antioxidants, 13(4), 431.

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Biochemical Assays for Antioxidant and Antidiabetic Evaluation

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Methanol, acetonitrile, and acetic acid were purchased from Merck (Darmstadt, Hesse, Germany) and TEDIA Company Inc. (Fairfield, OH, USA). High-purity water was acquired from Hangzhou Wahaha Group (Hangzhou, China). The remaining chemical reagents were purchased from Sinopharm Co. Ltd. (Shanghai, China). P-nitrophenyl-α-D glucopyranoside (pNPG), 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS+), acarbose, glucose (GLU), alloxan (ALX), and L(+)-ascorbic acid (Vc) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd., (Shanghai, China).

Li M., Xie G., Ding Y., Ma J., Liu Q., Wang Y., Peng Z., Sun J, & Shang J. (2023). Chemometric Discrimination of Cichorium glandulosum Boiss. et Huet and Cichorium intybus L. via Their Metabolic Profiling, Antioxidative, and Hypoglycemic Activities. Foods, 12(4), 901.

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Safflower Seed Oil Antioxidant Evaluation

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Safflower seed crude oil was gifted from Yaquina Agricultural Development Co., Ltd. (Yining, China). α-Tocopherol and stigmasterol (standard, ≥95%) were purchased from Sigma-Aldrich (Shanghai, China); 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS, ≥98%), 1,1-diphenyl-2-picrylhydrazyl (DPPH, ≥98%), and Trolox (≥98%) were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Folin–Ciocalteu reagent was purchased from Solarbio Technology Co., Ltd. (Beijing, China); petroleum ether, isopropanol, glacial acetic acid, isooctane, absolute ethanol, sodium hydroxide, methanol, n-hexane, and other chemical reagents used were of either analytical or chromatographic grade and were purchased from Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China).

Xin L., Guo L., Edirs S., Zhang Z., Cai C., Yang Y., Lian Y, & Yang H. (2022). An Efficient Deacidification Process for Safflower Seed Oil with High Nutritional Property through Optimized Ultrasonic-Assisted Technology. Molecules, 27(7), 2305.

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Antioxidant Properties of Lotus Leaves

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Fresh lotus leaves were purchased from Ganzhou, Jiangxi province, China. Space lotus-36 was cultivated in a sheltered and sunny paddy field with fertile soil and sufficient water. The thickness of the soil layer was approximately 30 cm. The lotus root seed was planted in March. The tender lotus leaves were grown for 2–3 days, whereas the mature lotus leaves grown for 5–7 days. 1,1-diphenyl-2-picryl-hydrazyl (DPPH, CAS 1898-66-4) radical, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS⁺, CAS 30931-67-0) radical, gallic acid monohydrate (CAS 149-91-7), rutin (CAS 153-18-4) and Folin–Ciocalteu reagent (CAS 12111-13-6) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Other chemicals of analytically purity were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).

Ma Z., Ma Y., Liu Y., Zhou B., Zhao Y., Wu P., Zhang D, & Li D. (2023). Effects of Maturity and Processing on the Volatile Components, Phytochemical Profiles and Antioxidant Activity of Lotus (Nelumbo nucifera) Leaf. Foods, 12(1), 198.

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Antioxidant and Enzyme Inhibition Assays

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Fresh, high-quality, and disease-free watermelons, pineapples, pumpkins, cabbages, maize, and carrots (mini species and normal species) were purchased from an online store (https://www.jd.com accessed on: 20 May 2020) (Figure 1). All fruits and vegetables were in the stage that could be directly processed and eaten by consumers. Gallic acid, catechin 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and Folin-Ciocalteu reagent, α-amylase (bacillus subtilis source), α-glucosidase, starch soluble, 3,5-dinitrosalicylic acid (DNS), and p-nitrophenyl-α-D-glucopyranoside (PNPG) were all purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals and reagents were of analytical grade and purchased from Xilong Scientific Co., Ltd. (Guangzhou, China).

Wang J., Ma T., Wang L., Lan T., Fang Y, & Sun X. (2021). Research on the Consumption Trend, Nutritional Value, Biological Activity Evaluation, and Sensory Properties of Mini Fruits and Vegetables. Foods, 10(12), 2966.

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Optimizing Okara Incorporation in Wheat Flour

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Wheat flour with a crude protein content of 12.2% (N × 6.25) was purchased from Yihai Kerry Grain Industry Co., Ltd. (Shanghai, China). Fresh okara with a crude protein content of 19.35 ± 0.56% (N × 6.25) was obtained from the Beverage and Food Management Center at Yangzhou University (Yangzhou, China). The okara was dried in an oven (DHG‐9148A, Shanghai Jinghong Experimental Equipment Co., Ltd., China) at 60°C for 6 h. The dried okara was then ground using a grinder (H7d, Xiamen Hehui Electronic Technology Co., Fujian, China) and sieved through an 80‐mesh screen. 1,1‐Diphenyl‐2‐picrylhydrazyl, α‐amylase, and diammonium 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonate) were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China), and starch glucosidase was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). All chemicals used were of analytical grade.

Cao Z., Zhou L., Gao S., Yang C., Meng X, & Liu Z. (2024). Effects of different amounts of okara on texture, digestive properties, and microstructure of noodles. Food Science & Nutrition, 12(5), 3433-3442.

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