Glutamic acid d5

Manufactured by Merck Group

Sourced in United States

Glutamic acid-d5 is a stable isotope-labeled amino acid. It is used as a reference standard and as a tracer compound in various analytical and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Gibberellic acid, by Merck Group (9 mentions) Jasmonic acid, by Merck Group (9 mentions) Thymine d4, by Merck Group (9 mentions) Testosterone d3, by Merck Group (8 mentions) 15n anthranilic acid, by Merck Group (7 mentions) Trans zeatin, by Merck Group (6 mentions) Methanol, by Avantor (5 mentions) Sarcosine d3, by Merck Group (5 mentions) N acetyl aspartic acid d3, by Merck Group (5 mentions) Tryptophan 15n2, by Merck Group (5 mentions) Hplc grade acetonitrile, by Avantor (3 mentions) 15n 2 tryptophan, by Merck Group (3 mentions) Formic acid, by Merck Group (2 mentions) Chloroform, by Avantor (2 mentions) Ms grade formic acid, by Merck Group (2 mentions) Hplc grade ammonium acetate, by Avantor (2 mentions) Trans zeatine, by Merck Group (2 mentions) Acetonitrile trifluroacetic acid water, by Agilent Technologies (2 mentions) Succinic acid d4, by Merck Group (1 mentions) Valine d8, by Merck Group (1 mentions)

Close spelling variants of this product

L-glutamic acid -2,3,3,4,4-d5 (2 citations) Glutamic acid (138 citations)

10 protocols using glutamic acid d5

Plasma Metabolomics Analysis Protocol

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma samples for metabolomics were acquired at baseline, 1 h from the start of the clamp and 20 min after the last infusion (50 ng adrenaline kg⁻¹ min⁻¹) using containers containing heparin. Plasma samples (30 μl) were mixed with 400 μl methanol, 10 μl internal standard mixture succinic acid-d4, glutamic acid-d5, valine-d8 and heptadecanoic acid-d33, Sigma Aldrich, USA), and incubated on ice for 30 min. Samples were then centrifuged (10,000 g, 3 min, 4°C), and 180 μl of the filtered extracts were evaporated to dryness and derivatised. A detailed description of the process has been presented previously [13 (link)]. A 7250 GC/Q-TOF instrument (Agilent, USA) equipped with a Gerstel MPS autosampler (Gerstel, Germany) was used to analyse the samples. Features from the quantitative ion peak areas of the data were matched against mass spectral libraries (in-house library, Fiehn library [14 (link)], GOLM DB [15 (link)], GNPS [16 (link)], HMDB [17 (link)] and MassBank Japan [18 (link)]).

She R., Suvitaival T., Andersen H.U., Hommel E., Nørgaard K., Wojtaszewski J.F., Legido-Quigley C, & Pedersen-Bjergaard U. (2024). Metabolic effect of adrenaline infusion in people with type 1 diabetes and healthy individuals. Diabetologia, 67(6), 1095-1106.

+ Open protocol

+ Expand

HPLC-MS Chemical Standards Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-performance liquid chromatography (HPLC) grade acetonitrile, methanol, and water were purchased from Burdick & Jackson (Morristown, NJ). The mass spectrometry grade formic acid and internal standards, including N-acetyl L-aspartic acid-d3, L-tryptophan-¹⁵N₂, sarcosine-d₃, glutamic acid-d₅, thymine-d₄, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d₃, were purchased from Sigma-Aldrich (St.Louis, MO). The ESI-L Low Concentration Tuning Mix (Agilent Technologies, Santa Clara, CA) was used to calibrate the mass spectrometer.

Jiao L., Maity S., Coarfa C., Rajapakshe K., Chen L., Jin F., Putluri V., Tinker L.F., Mo Q., Chen F., Sen S., Sangi-Hyghpeykar H., El-Serag H.B, & Putluri N. (2019). A prospective targeted serum metabolomics study of pancreatic cancer in postmenopausal women. Cancer prevention research (Philadelphia, Pa.), 12(4), 237-246.

+ Open protocol

+ Expand

Comprehensive Metabolite Analysis by HPLC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-performance liquid chromatography (HPLC)-grade ammonium acetate from Sigma, acetonitrile, methanol, chloroform, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO).) Metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).

Amara C.S., Ambati C.R., Vantaku V., Piyarathna D.W., Donepudi S.R., Ravi S.S., Arnold J.M., Putluri V., Chatta G., Guru K.A., Badr H., Terris M.K., Bollag R.J., Sreekumar A., Apolo A.B, & Putluri N. (2019). Serum metabolic profiling identified a distinct metabolic signature in Bladder Cancer Smokers: A key metabolic enzymes associated with patient survival. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(4), 770-781.

+ Open protocol

+ Expand

Metabolite Extraction and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC grade ammonium acetate, acetonitrile, methanol, chloroform, ethanol, hexane, and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standard compounds and isotope labeled standards, including N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).

Ambati C.R., Vantaku V., Donepudi S.R., Amara C.S., Ravi S.S., Mandalapu A., Perla M., Putluri V., Sreekumar A, & Putluri N. (2018). Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application. Analytical methods : advancing methods and applications, 11(1), 49-57.

+ Open protocol

+ Expand

Comprehensive Metabolomics Analytical Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sources for reagents were: HPLC-grade acetonitrile and water (Burdick & Jackson); mass spectrometry-grade formic acid and ammonium acetate (Sigma-Aldrich); calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water (Agilent Technologies); metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, Tryptophan-15N2, Sarcosine-d3, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, 15N Anthranilic acid, and Testosterone-d3 (Sigma-Aldrich).

Amobi-McCloud A., Muthuswamy R., Battaglia S., Yu H., Liu T., Wang J., Putluri V., Singh P.K., Qian F., Huang R.Y., Putluri N., Tsuji T., Lugade A.A., Liu S, & Odunsi K. (2021). IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation. Frontiers in Immunology, 12, 678999.

+ Open protocol

+ Expand

HPLC-MS Metabolite Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-performance liquid chromatography (HPLC) grade acetonitrile, methanol and water were purchased from Burdick & Jackson (Morristown, NJ). Mass spectrometry grade formic acid and internal standards namely, Tryptophan-15N2, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatin, Jasmonic acid, Anthranilic acid, and Testosterone-d3 were purchased from Sigma- Aldrich (St.Louis, MO). The calibration solution containing multiple calibrants in acetonitrile/trifluroacetic acid /water was purchased from Agilent Technologies (Santa Clara, CA).

Wangler M.F., Chao Y.H., Bayat V., Giagtzoglou N., Shinde A.B., Putluri N., Coarfa C., Donti T., Graham B.H., Faust J.E., McNew J.A., Moser A., Sardiello M., Baes M, & Bellen H.J. (2017). Peroxisomal biogenesis is genetically and biochemically linked to carbohydrate metabolism in Drosophila and mouse. PLoS Genetics, 13(6), e1006825.

+ Open protocol

+ Expand

Metabolomic Analysis using LC-MS

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-performance liquid chromatography (HPLC) grade acetonitrile, methanol and water were purchased from Burdick & Jackson, NJ. Mass spectrometry grade formic acid was purchased from Sigma- Aldrich, (St Louis, MO). Internal standards namely, [15N]2-Tryptophan, Glutamic acid –d5, Gibberellic acid, Jasmonic acid, Thymine-d4, and Zeatine, were purchased from Sigma- Aldrich, (St Louis, MO). Another internal standard, [15N] Anthranilic acid was purchased from Cambridge Isotope, (Tewksbury, MA). The calibration solution containing multiple calibrants in acetonitrile/trifluroacetic acid/water was purchased from Agilent Technologies, (Santa Clara, CA). The metabolomic analyses of all samples were executed using the protocol described previously [21 (link), 27 (link)–31 (link)]. The raw data (LC-MS output) was normalized using internal standards.

Bhowmik S.K., Ramirez-Peña E., Arnold J.M., Putluri V., Sphyris N., Michailidis G., Putluri N., Ambs S., Sreekumar A, & Mani S.A. (2015). EMT-induced metabolite signature identifies poor clinical outcome. Oncotarget, 6(40), 42651-42660.

+ Open protocol

+ Expand

HPLC-Based Quantification of Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents such as acetonitrile, methanol and water for high-performance liquid chromatography were purchased from Burdick and Jackson. Formic acid and internal standards, which included a mixture of Jasmonic acid, [¹⁵N]₂-Tryptophan, Thymine-d4, Glutamic acid-d5, Gibberellic acid, Trans-Zeatin, [¹⁵N]-Anthranilic acid and Testosterone-d3, were purchased from Sigma-Aldrich. For UDP-N-Acetyl glucosamine quantification, [¹³C]₆ N-acetyl glucosamine was used as an internal standard and the calibration curve for quantification was generated using the purified metabolite purchased from Sigma-Aldrich.

Kaushik A.K., Shojaie A., Panzitt K., Sonavane R., Venghatakrishnan H., Manikkam M., Zaslavsky A., Putluri V., Vasu V.T., Zhang Y., Khan A.S., Lloyd S., Szafran A.T., Dasgupta S., Bader D.A., Stossi F., Li H., Samanta S., Cao X., Tsouko E., Huang S., Frigo D.E., Chan L., Edwards D.P., Kaipparettu B.A., Mitsiades N., Weigel N.L., Mancini M., McGuire S.E., Mehra R., Ittmann M.M., Chinnaiyan A.M., Putluri N., Palapattu G.S., Michailidis G, & Sreekumar A. (2016). Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer. Nature Communications, 7, 11612.

+ Open protocol

+ Expand

Comprehensive Metabolite Profiling Approach

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-performance liquid chromatography (HPLC)-grade ammonium acetate, acetonitrile, methanol, chloroform and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standards and internal standards, N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, anthranilic acid-15N, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO). Mixture of LPC 17:0/0:0, PG 17:0/17:0, PE 17:0/17:0, PC 17:0/17:0, TAG 17:0/17:0/17:0, SM 18:1/17:0, MAG 17:0, DAG 16:0/18:1, CE 17:0, ceramide 18:1/17:0, PA 17:0, PI 17:0/20:4, and PS 17:0/17:0. were obtained from Avanti polar lipids (USA) (Supplementary Table S1).

Vantaku V., Dong J., Ambati C.R., Perera D., Donepudi S.R., Amara C.S., Putluri V., Shiva Shankar R., Robertson M.J., Piyarathna D.W., Villanueva M., von Rundstedt F.C., Karanam B., Ballester L.Y., Terris M.K., Bollag R.J., Lerner S.P., Andrea A.B., Villanueva H., Lee M., Sikora A.G., Lotan Y., Sreekumar A., Coarfa C, & Putluri N. (2019). Multi-omics integration analysis robustly predicts high-grade patient survival and identifies CPT1B effect on fatty acid metabolism in Bladder Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(12), 3689-3701.

+ Open protocol

+ Expand

Metabolomic Profiling of Eosinophilic Esophagitis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Using mass spectrometry, 48 metabolites were measured and compared between children with active EoE and unaffected controls.
Metabolites and internal standards, including N-acetyl aspartic acid-d3, [15N]2 Tryptophan, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, [15N] Anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).

Moye L.M., Liu Y., Coarfa C., Putluri N, & Rhoads J.M. (2019). Plasma Urea Cycle Metabolites May Be Useful Biomarkers in Children With Eosinophilic Esophagitis. Frontiers in Pediatrics, 6, 423.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!