If not otherwise stated, all materials were purchased in the highest possible quality from Sigma-Aldrich (St. Louis, MO, USA). Trypsin (sequencing grade) was obtained from Roche Diagnostic GmbH (Mannheim, Germany). Water was used after purification with a Milli Q-8 direct system (Merck KGaA, Darmstadt, Germany). IgG (I4506) and A1PI (A6150) were obtained from Sigma-Aldrich. Human serum was obtained from BioreclamationIVT (New York, USA). The amino acid numbering applied for all proteins analysed in this study is based on the respective UniProtKB entries.
Milli q direct 8 system
Manufactured by Merck Group
Sourced in Italy, Germany, United States
The Milli-Q Direct 8 system is a water purification device designed to produce ultrapure water for various laboratory applications. It utilizes a combination of technologies, including reverse osmosis and ion exchange, to remove impurities and deliver high-quality water with low levels of organic and inorganic contaminants.
Automatically generated - may contain errors
Lab products found in correlation
Chymotrypsin, by Merck Group (2 mentions)
Pancreatin, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Propranolol hydrochloride, by Merck Group (2 mentions)
Dibenzylamine, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Honeywell (2 mentions)
Methacrylic acid, by Merck Group (2 mentions)
Anhydrous sodium carbonate, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Sequencing grade trypsin, by Roche (1 mentions)
Igg i4506, by Merck Group (1 mentions)
Trypsin, by Roche (1 mentions)
Trifluoroacetic acid tfa, by Iris Biotech (1 mentions)
Rink amide chemmatrix resin, by Biotage (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Small molecules, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Avantor (1 mentions)
Lc ms grade acetonitrile acn, by Avantor (1 mentions)
Prestoblue cell viability assay, by Thermo Fisher Scientific (1 mentions)
24 protocols using milli q direct 8 system
1
Protein Quantification Protocol
2
Immunoglobulin Characterization Protocol
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If not otherwise stated, all materials were purchased in high quality from Sigma-Aldrich (St. Louis, MO, USA). Trypsin (sequencing grade) was obtained from Roche Diagnostic GmbH (Mannheim, Germany). Water was used after purification with a Milli Q-8 direct system (Merck KGaA, Darmstadt, Germany). IgA (plasma), sIgA (human colostrum), IgD (plasma), IgE (myeloma plasma, lambda), IgG1 (myeloma plasma, kappa), IgG2 (myeloma plasma, kappa), and IgM (plasma) were obtained from Athens Research & Technology (Athens, GA, USA). The amino acid numbering applied for all proteins analyzed in this study is based on the respective UniProtKB entries.
3
Solid-Phase Peptide Synthesis Protocol
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N-α-Fmoc-protected amino acids, coupling reagents 1-Hydroxy-7-azabenzotriazole (HOAt) and 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluoro-phosphate (HBTU), N, N-Diisopropylethylamine (DIEA), piperidine and trifluoroacetic acid (TFA) were purchased from Iris Biotech (Germany). Rink Amide-ChemMatrix resin was purchased from Biotage AB (Sweden). Peptide synthesis solvents, reagents, as well as CH3CN for High Performance Liquid Chromatography (HPLC) were reagent grade and were acquired from commercial sources and used without further purification unless otherwise noted. Ultrapure water (H2O) was obtained by a Direct-8 Milli-Q system (Millipore, Milan, Italy).
4
Quantification of Carotenoids in Spirulina
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Ultra-pure water (H2O) was obtained by a Direct-8 Milli-Q system (Millipore, Milan, Italy); LC-MS grade acetonitrile (ACN), methanol (CH3OH), 2-propanol (IPA), water (H2O), ammonium acetate (CH3COONH4), and standards of β-carotene, lutein, and zeaxanthin, were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Spirulina powders and tablets were respectively purchased from FarmaLabor SRL (Canosa di Puglia, Barletta-Trani, Italy) and Dr. Giorgini (Bologna, Italy). Lab-made Spirulina powder was kindly donated by a local farmer.
5
Optimized Extraction of Hop Compounds
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Ultra-pure water (H 2 O) was obtained using a Direct-8 Milli-Q system (Millipore, Milan, Italy); LC-MS grade acetonitrile (ACN) and water (H 2 O), methanol (CH 3 OH) and the additives formic acid (CH 3 OOH) and trifluoroacetic acid (TFA) were all purchased from Sigma-Aldrich (St Louis (MO), USA). Hop pellets (Target T90) were kindly donated by a local brewery company (AEFFE, Castel San Giorgio, Salerno, Italy). All other powders and inhibitors were purchased from Sigma-Aldrich.
6
Spirulina Powder Bioactivity Analysis
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Spirulina platensis powder was obtained from FarmaLabor Srl (Canosa di Puglia, Barletta-Trani, Italy).
Ultra-pure water (H2O) was obtained by a Direct-8 Milli-Q system (Millipore, Milan, Italy); LC-MS grade acetonitrile (ACN) and water (H2O), formic acid (HCOOH), trifluoroacetic acid (TFA), hydrochloric acid, ammonium formate (HCOONH4) pepsin, chymotrypsin (bovine pancreas), pancreatin (porcine pancreas), bile salts, Chitosan (C) low molecular weight (LMW ≈150 kDa), degree of deacetylation ≥75% were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Mannitol (M) was purchased from Carlo Erba Reagenti (Rodano, Milano, Italy). Permeapad® barriers were kindly gifted by InnoME GmbH (Espelkamp, Germany). Peptide SP6 (GIVAGDVTPI) was synthesized as reported by Carrizzo et al., 2019 [9 (link)], and kindly provided by the authors.
Ultra-pure water (H2O) was obtained by a Direct-8 Milli-Q system (Millipore, Milan, Italy); LC-MS grade acetonitrile (ACN) and water (H2O), formic acid (HCOOH), trifluoroacetic acid (TFA), hydrochloric acid, ammonium formate (HCOONH4) pepsin, chymotrypsin (bovine pancreas), pancreatin (porcine pancreas), bile salts, Chitosan (C) low molecular weight (LMW ≈150 kDa), degree of deacetylation ≥75% were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Mannitol (M) was purchased from Carlo Erba Reagenti (Rodano, Milano, Italy). Permeapad® barriers were kindly gifted by InnoME GmbH (Espelkamp, Germany). Peptide SP6 (GIVAGDVTPI) was synthesized as reported by Carrizzo et al., 2019 [9 (link)], and kindly provided by the authors.
7
Comprehensive Analytical Protocol for Complex Biological Samples
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Ultra pure water (H 2 O) was obtained by a Direct-8 Milli-Q system (Millipore, Milan, Italy), LC-MS grade acetonitrile (ACN) and additives formic acid (HCOOH), ammonium formate (HCOONH 4 ) phosphoric acid (H 3 PO 4 ) and Trifluoroacetic acid (TFA) were all purchased by VWR (Milan, Italy). Enzymes Pepsin, Chymotrypsin, Pancreatin, bile salts, small molecules and peptide standard mix were all purchased by Sigma Aldrich (Milan, Italy). Klamath and Spirulina nutraceutical formulations (powders) were obtained by Farmalabor (Assago, Milan, Italy).
8
Evaluation of Antiviral Activity and Cytotoxicity
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Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich and used without further purification. RBV was purchased from APIChem (China). Milli-Q water (resistivity 18.2 MΩ cm) was obtained from a Millipore Milli-Q Direct 8 system. Trypsin–EDTA 0.05%, fetal bovine serum (FBS, < 5 EU mL–1) and Presto Blue cell viability assay were purchased from Invitrogen. Cell Counting-Kit CCK-8 was purchased from Sigma-Aldrich. Britelite plus reagent for measuring activity of luciferase reporter system was purchased from Perkin-Elmer. RAW264.7 (murine monocyte macrophage cell line, ATCC) and TZM-bl cells (obtained from NIH AIDS Reagent Program, catalogue no. 8129) were routinely cultured in full culture medium containing Dulbecco's modified Eagle medium (DMEM), FBS (10%) and penicillin/streptomycin (1%) at 37 °C with 5% CO2.
9
HPLC Characterization of Peptide Coatings
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Analytical HPLC was performed on a Shimadzu LC-2010A HT equipped with a Ascentis® Express Peptide ES-C18 column with 2.7 μm particles, a length of 150 mm and an internal diameter of 3.0 mm from Supelco Analytical. HPLC mobile phase A was ultrapure H2O supplemented with 0.1% TFA (v/v) and mobile phase B acetonitrile (MeCN) supplemented with 0.1% TFA (v/v). Ultrapure water (MQ) was obtained from Milli Q direct 8 system (Millipore). Polymers used for SMEPT coatings were poly(ethyleneimine) (PEI 25 kDa, branched), poly(sodium-4-styrene sulfonate) (PSS, 70 kDa), poly(allylamine hydrochloride) (PAH, 17 kDa).
10
Ultrapure Water Preparation Protocol
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Ultra pure water (H2O) was from a Milli-Q Direct 8 system (Millipore, Milan, Italy). Unless otherwise specified, all reagents and compounds were purchased from Sigma Chemicals Company (Sigma, Milan, Italy).
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