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2 20 azino bis 3 ethyl benzo thiazoline 6 sulphonic acid

Manufactured by Merck Group
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Sourced in Germany, United States

2,20 -azino-bis [3-ethyl benzo thiazoline-6-sulphonic acid] is a chemical compound that can be used as a reagent in various laboratory applications. It functions as a chromogenic substance, capable of undergoing color changes in certain reactions. The core purpose of this product is to serve as a detection and quantification tool for various analytical techniques.

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Lab products found in correlation

Ascorbic acid, by Merck Group (3 mentions) Ethanol, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Methanol, by Merck Group (2 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions) Potassium persulfate, by Merck Group (2 mentions) 1 1 diphenyl 2 picrylhydrazyl, by Merck Group (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Chloroform, by Merck Group (2 mentions) Folin ciocalteu reagent, by Merck Group (2 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Osmium tetroxide, by Merck Group (1 mentions) Zinc nitrate hexahydrate, by Merck Group (1 mentions) Glutaraldhyde, by Merck Group (1 mentions) Potassium persulfate k2s2o8, by Merck Group (1 mentions) Potassium hydroxide, by Merck Group (1 mentions) N butanol, by Merck Group (1 mentions) Resazurin, by Merck Group (1 mentions) Muller hinton broth, by HiMedia (1 mentions) Muller hinton agar, by HiMedia (1 mentions)

Close spelling variants of this product

2,2′-azino-bis (284 citations) 2,20-azino-bis (11 citations)

5 protocols using 2 20 azino bis 3 ethyl benzo thiazoline 6 sulphonic acid

1

Preparation and Characterization of Nanoparticles

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Zinc nitrate hexahydrate (98%), dimethylsulfoxide (DMSO, 96%), ascorbic acid (99%), osmium tetroxide (99.8%), glutaraldhyde (50% in water), phosphate-buffered saline (pH 7.4), ethanol (98%), methanol (99.85%), potassium persulfate (98.5%), 2,2-diphenyl-1-picrylhydrazyl (DPPH, 95%) and 2,20 -azino-bis [3-ethyl benzo thiazoline-6-sulphonic acid] (ABST, 98%) were purchased from Sigma-Aldrich (Hamburg, Germany).
Al-Mohaimeed A.M., Al-Onazi W.A, & El-Tohamy M.F. (2022). Multifunctional Eco-Friendly Synthesis of ZnO Nanoparticles in Biomedical Applications. Molecules, 27(2), 579.
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2

Antioxidant and Antimicrobial Evaluation

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All the chemicals and reagents used for this study including; ethanol (96%), methanol (99.8%), petroleum ether (40–60 °C), dichloromethane (≥99.8%), chloroform (99.9%), n-butanol (99.8%), dimethyl sulfoxide (DMSO), hydrochloric acid (HCl), potassium hydroxide (KOH, ≥85%), 1,1-diphenyl2-picrylhydrazyl (DPPH, 95%), 2, 20-azino-bis[3-ethyl benzo thiazoline-6-sulphonic acid] (ABTS), potassium persulfate (K2S2O8), acetylthiocholine iodide (≥99.0%), S-butyrylthiochoilne iodide (≥98.0%), sodium phosphate (Na3PO4, 96%), ascorbic acid, resazurin, 2-nitrobenzoic acid and were obtained from Sigma Aldrich (Hamburg, Germany). Muller Hinton agar and Muller Hinton broth were procured from HiMedia (Himedia Laboratories Pvt Ltd., Mumbai, India). Donepezil, galantamine and chloramphenicol were purchased from the local drug store.
A. Al-Hamoud G., Al-Musayeib N.M., Amina M, & Ibrahim S.R. (2022). Abubidentin A, New Oleanane-type Triterpene Ester from Abutilon bidentatum and its antioxidant, cholinesterase and antimicrobial activities. PeerJ, 10, e13040.
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3

Antioxidant Compound Extraction and Analysis

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Gallic acid, Folin–ciocalteu reagent, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,20-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulphate, quercetin, trans-resveratrol, ascorbic acid, phenylephrine (Phe), and acetylcholine chloride, were purchased from Sigma–Aldrich (St. Louis, MO, USA). Ethyl acetate and HPLC-grade acetonitrile was from Tedia (Tedia, Brazil). Water was purified through a Milliq1 water purification system (Millipore, Billerica, MA, USA) and the other solvents were all reagent-grade.
de Figueiredo E.A., Alves N.F., Monteiro M.M., Cavalcanti C.D., da Silva T.M., da Silva T.M., Braga V.D, & Oliveira E.D. (2017). Antioxidant and Antihypertensive Effects of a Chemically Defined Fraction of Syrah Red Wine on Spontaneously Hypertensive Rats. Nutrients, 9(6), 574.
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4

Ostrich Lung ACE Purification and Antioxidant Analysis

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Ostrich lungs were prepared from Mashhad Meat Industrial Complex (Mashhad, Iran), and ACE from ostrich lung was purified as previously reported [14 ]. Fresh ostrich egg was prepared from Esteghlal market (Mashhad, Iran). Pepsin (porcine gastric mucosa, EC 3.4.23.1), pancreatin (porcine pancreas), 1,1-diphenyl-2-picrylhydrazyl (DPPH), glutathione (GSH), 2,20-azinobis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS), potassium persulfate, potassium phosphate, trichloroacetic acid (TCA), ferric chloride, 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ethylenediaminetetra-acetic acid (EDTA), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ultrafiltration membranes with a 3-kDa cut off were purchased from Millipore (Bedford, MA, USA).
Asoodeh A., Homayouni-Tabrizi M., Shabestarian H., Emtenani S, & Emtenani S. (2016). Biochemical characterization of a novel antioxidant and angiotensin I-converting enzyme inhibitory peptide from Struthio camelus egg white protein hydrolysis. Journal of Food and Drug Analysis, 24(2), 332-342.
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5

Comprehensive Phytochemical Analysis

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All used solvents (chloroform, methanol, hexane and dimethyl sulphoxide) were purchased from Sigma Aldrich, such as Folin-Ciocalteu reagent, gallic and ascorbic acids, iron(III) hloride hexahydrate, 2,2-Diphenyl-1-picrylhydrazyl, 2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid and 12% methanolic solution of boron trifluoride. Chemical substances of analytical grade obtained from Merck were: neocuproine, copper(II) chloride dihydrate, sodium carbonate, hydrochloric acid, 2,4,2-tri(2-pyridyl)-s-triazine, 10 % HNO3 was used for soaking all glassware which were rinsed well with distilled water.
Dimitrijevic M.V., Mitic V.D., Nikolic J.S., Djordjevic A.S., Mutic J.J., Stankov Jovanovic V.P, & Stojanovic G.S. (2019). First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. Chemistry & biodiversity, 16(2).
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