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Anti eomes dan11mag and live dead aqua

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Anti-Eomes (Dan11mag) is a monoclonal antibody used in flow cytometry to detect the transcription factor Eomes. Live/Dead Aqua is a fluorescent dye used to distinguish live and dead cells in flow cytometry applications.

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Lab products found in correlation

Anti cd49b dx5, by BD (3 mentions) Facsjazz, by BD (3 mentions) Lsrfortessa, by BD (3 mentions) Anti rorγt b2d, by Thermo Fisher Scientific (2 mentions) Anti cd8β yts156.7.7, by BioLegend (2 mentions) Anti γδ tcr ebiogl3, by Thermo Fisher Scientific (2 mentions) Anti rorgt b2d, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Live/Dead Aqua (379 citations) Eomes (10 citations) Eomes (Dan11mag) (6 citations) Anti-Eomes (Dan11mag) (5 citations) Dan11mag (5 citations) Anti-Eomes (4 citations)

3 protocols using anti eomes dan11mag and live dead aqua

1

Isolation and Characterization of Intestinal Cell Populations

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Single-cell suspensions were prepared from intestinal sections by shaking diced tissue at 37C for 25 minutes in Hank’s Balanced Salt Solution with 5 mM EDTA and 1 mM DTT. Cell pellets were then passed through 70 mm and 40 mm filters. Cells were surface stained using the following fluorochrome-conjugated Abs: anti-EpCAM (G8.8), anti-CD45.2 (104), anti-CD19 (MB19-1), anti-Thy1.1 (HIS51), anti-CD27 (LG.7F9), anti-RORγt (B2D) and anti-γδ TCR (eBioGL3) from eBioscience; anti-NK1.1 (PK136), anti-NKp46 (29A1.4), anti-CD3 (17A2), anti-CD127 (SB/199), anti-CD49a (HMα1), anti-T-bet (4B10) and anti-CD8β (YTS156.7.7) from BioLegend; anti-Eomes (Dan11mag) and Live/Dead Aqua from Invitrogen; and anti-CD49b (DX5) from BD Biosciences. Data were collected on a LSRFortessa (BD Biosciences) and analyzed with FlowJo v10 software (TreeStar). Cell sorting was performed on a BD FACS Jazz (BD Biosciences).
Gullicksrud J.A., Sateriale A., Englies J.B., Gibson A., Shaw S., Hutchins Z.A., Martin L., Christian D.A., Taylor G.A., Yamamoto M., Beiting D.P., Striepen B, & Hunter C.A. (2021). Enterocyte–innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium. Mucosal immunology, 15(2), 362-372.
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2

Isolation and Characterization of Intestinal Cell Populations

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Single-cell suspensions were prepared from intestinal sections by shaking diced tissue at 37C for 25 minutes in Hank’s Balanced Salt Solution with 5 mM EDTA and 1 mM DTT. Cell pellets were then passed through 70 mm and 40 mm filters. Cells were surface stained using the following fluorochrome-conjugated Abs: anti-EpCAM (G8.8), anti-CD45.2 (104), anti-CD19 (MB19-1), anti-Thy1.1 (HIS51), anti-CD27 (LG.7F9), anti-RORγt (B2D) and anti-γδ TCR (eBioGL3) from eBioscience; anti-NK1.1 (PK136), anti-NKp46 (29A1.4), anti-CD3 (17A2), anti-CD127 (SB/199), anti-CD49a (HMα1), anti-T-bet (4B10) and anti-CD8β (YTS156.7.7) from BioLegend; anti-Eomes (Dan11mag) and Live/Dead Aqua from Invitrogen; and anti-CD49b (DX5) from BD Biosciences. Data were collected on a LSRFortessa (BD Biosciences) and analyzed with FlowJo v10 software (TreeStar). Cell sorting was performed on a BD FACS Jazz (BD Biosciences).
Gullicksrud J.A., Sateriale A., Englies J.B., Gibson A., Shaw S., Hutchins Z.A., Martin L., Christian D.A., Taylor G.A., Yamamoto M., Beiting D.P., Striepen B, & Hunter C.A. (2021). Enterocyte–innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium. Mucosal immunology, 15(2), 362-372.
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3

Single-Cell Isolation from Intestine

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Single-cell suspensions were prepared from intestinal sections by shaking diced tissue at 37C for 25 minutes in Hank's Balanced Salt Solution with 5 mM EDTA and 1 mM DTT. Cell pellets were then passed through 70 mm and 40 mm filters. Cells were surface stained using the following fluorochrome-conjugated Abs: anti-EpCAM (G8.8), anti-CD45.2 (104), anti-CD19 (MB19-1), anti-Thy1.1 (HIS51), anti-CD27 (LG.7F9), anti-RORgt (B2D) and anti-gd TCR (eBioGL3) from eBioscience; anti-NK1.1 (PK136), anti-NKp46 (29A1.4), anti-CD3 (17A2), anti-CD127 (SB/199), anti-CD49a (HMa1), anti-T-bet (4B10) and anti-CD8b (YTS156.7.7) from BioLegend; anti-Eomes (Dan11mag) and Live/Dead Aqua from Invitrogen; and anti-CD49b (DX5) from BD Biosciences. Data were collected on a LSRFortessa (BD Biosciences) and analyzed with FlowJo v10 software (TreeStar) . Cell sorting was performed on a BD FACS Jazz (BD Biosciences).
Gullicksrud J.A., Sateriale A., Englies J., Gibson A.R., Shaw S., Hutchins Z., Martin L.E., Christian D.A., Taylor G.A., Yamamoto M., Beiting D.P., Striepen B., & Hunter C.A. (2021). Crosstalk between enterocytes and innate lymphoid cell drives early IFN-γ-mediated control ofCryptosporidium.
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