After 18 dye + light treatments or 16 h treatment with 0.4 μM APH, cells were allowed to recover for 8h prior to an 8h incubation with 7 μM Cdk1 inhibitor RO3306 (Millipore). Where indicated, cells were incubated with MRE11 inhibitor Mirin (100 μM; Sigma) or RAD51 inhibitor B02 (100 μM; Sigma) during the last hour of Cdk1 inhibitor incubation. Cells were washed twice with PBS and incubated in fresh media containing 1× EdU and 50ng/ml concentrations colcemid with or without MRE11 or RAD51 inhibitor for 1h prior to harvest. Metaphase spreads were prepared as described above and EdU staining performed using Click-iT™ EdU Alexa Fluor™ 594 imaging kit (ThermoFisher) after telomere FISH staining.
Rad51 inhibitor b02
Manufactured by Merck Group
Sourced in United States
RAD51 inhibitor B02 is a chemical compound that functions as an inhibitor of the RAD51 protein. RAD51 is a key player in the homologous recombination DNA repair pathway. The RAD51 inhibitor B02 disrupts this pathway by targeting and inhibiting the RAD51 protein.
Automatically generated - may contain errors
Lab products found in correlation
Ro 3306, by Merck Group (1 mentions)
Click it edu alexa fluor 594 imaging kit, by Thermo Fisher Scientific (1 mentions)
Mirin, by Merck Group (1 mentions)
Ab63801, by Abcam (1 mentions)
M iggκ bp hrp sc 516102, by Santa Cruz Biotechnology (1 mentions)
γh2ax antibody, by Merck Group (1 mentions)
Anti mouse alexa 488 4408s, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp 7074, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Isorhamnetin, by Merck Group (1 mentions)
2 protocols using rad51 inhibitor b02
1
Induction of Cell Cycle Arrest and DNA Damage
2
Quantifying DNA Damage and Repair
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quercetin, isorhamnetin, B[a]P, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), RAD51 inhibitor B02, dimethyl sulfoxide, and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). The RAD51 antibody was purchased from abcam (ab63801, Cambridge, UK), and γH2AX antibody purchased from Millipore (05-636, Burlington, MA, USA). Anti-mouse Alexa 488 (4408S) and Anti rabbit IgG-HRP (7074S) was purchased from cell signaling technology (Danvers, MA, USA). The antibodies of goat anti-rabbit IgG−tRICT (sc-3841), beta-actin (sc 47778), and m-IgGκ BP-HRP (sc-516102) were purchased from Santa Cruz biotechnology (Dallas, TX, USA)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!