Optimem glutamax

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

OptiMEM GlutaMAX is a culture medium designed for the growth and maintenance of a variety of cell types. It is a modified version of the original OptiMEM medium, which incorporates a stabilized form of L-glutamine, known as GlutaMAX. The core function of OptiMEM GlutaMAX is to provide a reliable and consistent growth environment for cell cultures.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

OptiMEM medium with Glutamax OptiMEM glutaMax media GlutaMAX

50 protocols using optimem glutamax

Intracellular α-synuclein Aggregation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

H4 neuroglioma cells from human (origin) were cultured in Opti-MEM + GlutaMAX (Invitrogen, 51985-034) supplemented with 10% fetal bovine serum (FBS; Gibco, 10100-147) at 37°C, passaged, and plated on chamber slides (Labted-II, Nalgen-Nunc, 154526) or glass cover slips. For intracellular α-syn aggregation experiments, H4 cells were seeded in 24-well plate (5 × 10⁴ cells/well) 24 h prior to transient transfection with SynT (C-terminal tagged form of WT α-syn) and synphilin-1 (Fig. S1). Equi-molar ratios of plasmids were mixed with FuGENE® 6 (Promega, E2691) at a 1:2 mass volume ratio, and incubated for 15 min before the complex of transfection reagent and plasmids was transfected into cells according to the manufacturer’s protocol (2 h transfection and 6 h recovery time). ALP modifiers were incubated during the last 24 h before fixation and processing for immunocytochemistry and toxicity assessments. Co-transfection with an empty backbone-vector [pPAGFP-C1, Addgene, 11910] and mock transfection was used as control. Rapamycin (200 ng/ml, Sigma Aldrich, R0395) was prepared in DMSO, chloroquine diphosphate salt (50 mM, CQ, Sigma Aldrich, C6628) and 3-methyladenine (10 mM, 3-MA, Sigma Aldrich, M9281) in water.

Wu J.Z., Ardah M., Haikal C., Svanbergsson A., Diepenbroek M., Vaikath N.N., Li W., Wang Z.Y., Outeiro T.F., El-Agnaf O.M, & Li J.Y. (2019). Dihydromyricetin and Salvianolic acid B inhibit alpha-synuclein aggregation and enhance chaperone-mediated autophagy. Translational Neurodegeneration, 8, 18.

+ Open protocol

+ Expand

Interrogating AhR Transcriptional Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibrobasts (MEF) (2 × 10⁵) were electroporated (230 V, 75 Ohm and 1,500 microfarads) with 2 μg WT or AhR mutant plasmid (H285A), (Q377A), (Y316A), (Y316A/Q377A) in Optimem/Glutamax (Invitrogen) with 0.8 μg firefly luciferase reporter pGudLuc1.1 plasmid, which contains a 480 bp fragment of the upstream enhancer region of the mouse Cyp1a1 gene—including four xenobiotic response elements—upstream of the firefly luciferase coding sequence. The pRL-TK (0,2 μg; Promega) reporter plasmid encoding Renilla luciferase was electroporated as an internal control of the transfection process. Cells were seeded in 24-well plates at a density of 2 × 10⁵ cells/ml. After 24 h at 37 °C, cells were stimulated for 6–8 h with increasing concentrations of 3-IAld. Since 3-IAld was dissolved in DMSO (present as 0.1% v/v of experimental medium), 0.1% of DMSO was chosen as a vehicle. Luciferase assays were performed using the dual luciferase reporter assay kit (Promega).

Zelante T., Paolicelli G., Fallarino F., Gargaro M., Vascelli G., De Zuani M., Fric J., Laznickova P., Kohoutkova M.H., Macchiarulo A., Dolciami D., Pieraccini G., Gaetani L., Scalisi G., Trevisan C., Frossi B., Pucillo C., De Luca A., Nunzi E., Spaccapelo R., Pariano M., Borghi M., Boscaro F., Romoli R., Mancini A., Gentili L., Renga G., Costantini C., Puccetti M., Giovagnoli S., Ricci M., Antonini M., Calabresi P., Puccetti P., Di Filippo M, & Romani L. (2024). A microbially produced AhR ligand promotes a Tph1-driven tolerogenic program in multiple sclerosis. Scientific Reports, 14, 6651.

+ Open protocol

+ Expand

Silencing PAK4 Induces Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were plated in 6-well plates for immunoblotting or T25 flasks for apoptosis assays. After 24 hours, cell monolayers at approximately 60% confluency were subjected to siRNA transfection. The human PAK4 siRNA used is a smart-pool RNA, with 5 reference sequences (ThermoFisher catalog S20135). The transfection mixture was prepared in Opti-MEM GlutaMax medium from Invitrogen (Carlsbad, CA, USA) with siRNA and Lipofectamine RNAiMAX according to the manufacturer’s protocol. The final concentration of siRNA oligonucleotides (scrambled or PAK4) added to the cells were 25 nM. The cells were cultured in the presence of transfection mixture for 24h. The transfection mixture was replaced by fresh DMEM medium the following day, and cell culture was pursued for an additional 48 hours. After the transfection, cells were collected for immunoblotting, or apoptosis assays.

Aboud O.A., Chen C.H., Senapedis W., Baloglu E., Argueta C, & Weiss R.H. (2016). Dual and specific inhibition of NAMPT and PAK4 by KPT-9274 decreases kidney cancer growth. Molecular cancer therapeutics, 15(9), 2119-2129.

+ Open protocol

+ Expand

Cell Culture and Synchronization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH 3T3 and HEK 293T cells obtained from ATCC (American Type Culture Collection, Manassas, VA, USA) were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in 5% CO₂. Cells were arrested in the M phase with 100 ng ml⁻¹ of nocodazole (Sigma, St Louis, MO, USA) and 10 μM of RO3306 (Enzo, Farmingdale, NY, USA) for 16 h. HEK 293T cells for lentiviral packaging were maintained in Opti-MEM-GlutaMAX (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum, 10 mM of sodium butyrate and 1% penicillin–streptomycin.

Yoo B.H., Kang D.S., Park C.H., Kang K, & Bae C.D. (2017). CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining centrosome integrity. Experimental & Molecular Medicine, 49(7), e354-.

+ Open protocol

+ Expand

Transfection and Oxidative Stress in H4 Neuroglioma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

H4 neuroglioma cells of human origin (ATCC, HTB-148) were maintained in Opti-MEM + GlutaMAX (51985-042, Invitrogen, Darmstadt, Germany) supplemented with 10% fetal calf serum (FCS; 10270-106, Invitrogen, Darmstadt, Germany) at 37°C. 24 h prior transfection cells were plated in 24-well plates (3.5 × 10⁴ cells/well) or in 6-well plates (2 × 10⁵ cells/well) and cultured in 0.5 ml or 2 ml medium, respectively. Calcium-phosphate transfection was performed as previously described [35 (link)]. Equimolar ratios of pcDNA3.1 plasmids encoding human WT, H50Q, and H50R aSyn were transfected under a cytomegalovirus promoter. Mock transfection without adding plasmid DNA served as control. The transfection efficiency was controlled by using flow cytometry. For the treatment experiments, H4 cells were exposed to different concentrations of HNE (50 – 3000 μM, Cayman Chemical Company, Ann Arbor, MI, USA) or H₂O₂ (100 - 500 μM, Merck, Darmstadt, Germany) 24 h after transfection for 12 h (HNE) or 24 h (H₂O₂), respectively. The stock solution of 64 mM HNE was prepared in ethanol. The stock solutions of HNE and H₂O₂ were freshly diluted in culture medium prior to treatment. Vehicle controls (ethanol or culture medium) were prepared in accordance to the highest HNE and H₂O₂ concentration used for the treatments.

Xiang W., Menges S., Schlachetzki J.C., Meixner H., Hoffmann A.C., Schlötzer-Schrehardt U., Becker C.M., Winkler J, & Klucken J. (2015). Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity. Molecular Neurodegeneration, 10, 8.

+ Open protocol

+ Expand

Culturing Human H4 Neuroglioma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

H4 neuroglioma cells of human origin (ATCC, HTB-148) were cultured in Opti-MEM + GlutaMAX (51985-042, Invitrogen) supplemented with 10% fetal calf serum (FCS; 10270-106, Invitrogen) at 37 °C and 5% CO₂. 3 × 10⁴ H4 cells/cm² were plated 48 h prior to analysis leading to a final confluency of 90%.

Menges S., Minakaki G., Schaefer P.M., Meixner H., Prots I., Schlötzer-Schrehardt U., Friedland K., Winner B., Outeiro T.F., Winklhofer K.F., von Arnim C.A., Xiang W., Winkler J, & Klucken J. (2017). Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress. Scientific Reports, 7, 42942.

+ Open protocol

+ Expand

Culturing Primary Mouse T-ALL Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary mouse T-ALL cells isolated from spleen were maintained on OP9 stromal cells in OptiMEM + GlutaMAX (Invitrogen) supplemented with 10% FBS, 5 ng/ml IL-7, penicillin and streptomycin, and 55 μM β-mercaptoethanol and passaged every 3–4 days onto a fresh feeder layer. OP9 cells were pre-treated with 7.5 ng/mL mitomycin C (Sigma) to prevent feeder cell division. Mouse ETP T-ALL (Treanor et al., 2014 (link)) and a T-ALL cell line generated by ENU mutagenesis carrying a Notch1 PEST domain mutation (G7084 (ins)C7085; generated in the lab of A. Ferrando, Columbia University) were maintained on OP9-DLL4 feeders. AMD3100 octahydrochloride hydrate (10 ug/mL in water; Sigma), AMD3465 hexahydrobromide (50 ug/mL in water; Tocris or Cayman Chemical) or vehicle were added to culture media at the start of the culture period.

Pitt L.A., Tikhonova A.N., Hu H., Trimarchi T., King B., Gong Y., Sanchez-Martin M., Tsirigos A., Littman D.R., Ferrando A., Morrison S.J., Fooksman D.R., Aifantis I, & Schwab S.R. (2015). CXCL12-producing vascular endothelial niches control acute T cell leukemia maintenance. Cancer cell, 27(6), 755-768.

+ Open protocol

+ Expand

Culturing Intestinal Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All culture media were purchased from Life Technologies/Invitrogen (Cergy-Pontoise, France). Caco-2/TC7 cells (Chantret et al., 1994 (link)) were cultured as previously described (Morel et al., 2008 (link)). SW480 and COS7 cells were cultured in high-glucose DMEM GlutaMAX I supplemented with 10% heat-inactivated fetal bovine serum (Eurobio/Abcys, Les Ulis, France) and with (SW480) or without (COS7) 1% nonessential amino acids. Nontumoral crypt-like human intestinal cells HIEC-6, kindly provided by J.-F. Beaulieu (Department of Anatomy and Cell Biology, University of Sherbrooke, Sherbrooke, Canada), were cultured in OPTIMEM-GlutaMAX supplemented with 5% fetal bovine serum, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies/Invitrogen), and 5 ng/ml EGF (BD Biosciences, Le Pont de Claix, France). Depending on experiments, cells were plated on 3-μm–pore size microporous polyethylene terephthalate filters (Transwell; Corning, Fisher, Illkirch, France), glass coverslips (Polylabo, Strasbourg, France), or plastic (Corning, Fisher).

Besnier L.S., Cardot P., Da Rocha B., Simon A., Loew D., Klein C., Riveau B., Lacasa M., Clair C., Rousset M, & Thenet S. (2015). The cellular prion protein PrPc is a partner of the Wnt pathway in intestinal epithelial cells. Molecular Biology of the Cell, 26(18), 3313-3328.

+ Open protocol

+ Expand

Establishment of Lymphoblastoid Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

LCLs, extensively used to analyze genotype/phenotype correlation, result from B lymphocytes of control individuals (2N) and non-related DS with (DS/AD) or without (DS) AD as previously mentioned [24 (link),25 (link)]. Cultures of LCLs were done in Opti-MEM/GlutaMax, with 1% penicillin and streptomycin (10,000 U/mL) and 5% fetal bovine serum (from a unique batch) (Invitrogen, Cergy, France), at 37 °C with 5% CO₂ in a humidified incubator.

Moreau M., Carmona-Iragui M., Altuna M., Dalzon L., Barroeta I., Vilaire M., Durand S., Fortea J., Rebillat A.S, & Janel N. (2022). DYRK1A and Activity-Dependent Neuroprotective Protein Comparative Diagnosis Interest in Cerebrospinal Fluid and Plasma in the Context of Alzheimer-Related Cognitive Impairment in Down Syndrome Patients. Biomedicines, 10(6), 1380.

+ Open protocol

+ Expand

Aortic Ring Assay for Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aortic Ring assay was performed as previously described (Baker et al., 2012 (link)). Briefly, aortae from 12-week-old-mice were dissected, cut into 0.5–1.0mm rings, and embedded in Collagen Type I (Millipore) gels in 96-well dishes. Gels were cultured with OptiMEM + Gluta Max (Invitrogen) + 2.5% FBS + Penicillin/Streptomycin + 30ng/mL VEGF (R&D Systems). Cultures were fed for the first time on day 3 and every other day thereafter. Gels were stained in well and transferred to slides for mounting and imaging.

Bambino K., Lacko L., Hajjar K.A, & Stuhlmann H. (2014). Epidermal Growth Factor-Like Domain 7 is a marker of the endothelial lineage and active angiogenesis. Genesis (New York, N.Y. : 2000), 52(7), 657-670.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!