Agilent’s Cation Test Mixture stock (100 ppm each of ammonium, potassium, calcium, sodium, magnesium) was diluted to 25 ppm with DI water and 250 ppm lead from 1000 ppm stock of lead (chloride salt) dissolved in DI water obtained from EMD Millipore Elix10 Water Purification System (Darmstadt, Ger-many). The standard mixture was filtered using a 0.22 μm PES syringe filter (Argos Technologies, Elgin, IL, USA). The running buffer or BGE (background electrolyte) consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether, 10% v/v MeOH with pH adjusted to 4.35 using 50% v/v acetic acid. All chemicals were dissolved in DI water. Buffer was filtered using 0.22 μm PES membrane syringe filter and degassed for 10 min using TA Instruments degassing station (New Castle, DE, USA) in 2 mL glass CE vials (Agilent Technologies) prior to runs.
Glass ce vials
Manufactured by Agilent Technologies
Sourced in United States
Glass CE vials are small containers used to hold samples or solutions for analysis in capillary electrophoresis (CE) instruments. They are designed to fit securely into the sample tray or carousel of a CE system. The glass material provides a chemically inert and durable housing for the contents.
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2 protocols using glass ce vials
1
Cation Separation and Quantification by CE
2
Cation Separation and Quantification by CE
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Agilent’s Cation Test Mixture stock (100 ppm each of ammonium, potassium, calcium, sodium, magnesium) was diluted to 25 ppm with DI water and 250 ppm lead from 1000 ppm stock of lead (chloride salt) dissolved in DI water obtained from EMD Millipore Elix10 Water Purification System (Darmstadt, Ger-many). The standard mixture was filtered using a 0.22 μm PES syringe filter (Argos Technologies, Elgin, IL, USA). The running buffer or BGE (background electrolyte) consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether, 10% v/v MeOH with pH adjusted to 4.35 using 50% v/v acetic acid. All chemicals were dissolved in DI water. Buffer was filtered using 0.22 μm PES membrane syringe filter and degassed for 10 min using TA Instruments degassing station (New Castle, DE, USA) in 2 mL glass CE vials (Agilent Technologies) prior to runs.
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