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Microarray assays were performed using Agilent’s genome-wide mouse expression array (GE 4x44K v2 two color microarray) slides and kits (Agilent Technologies, Inc., Santa Clara, CA) following the manufacturer’s protocol. Hybridized microarray slides were scanned using Agilent Technologies Scanner G2505C US09493743.

Dual dye microarray using the Whole Human Genome Microarray Kit (Agilent Technologies, Inc., Santa Clara, CA) was carried out following the vendor’s protocol. We labeled 12.5 ng amplified RNA with biotin and the same amount of reference RNA with fluorescein. Samples were hybridized to Agilent 4 × 44 k slides and incubated for 16 h at 55°C. A protocol with a series of washes was carried out for tagging Cy-3 and Cy-5 dyes to reference and sample RNA, respectively. The slides were scanned using Agilent Technologies Scanner G2505C US09493743 and feature extracted using the software v. 10.7 (Agilent, Inc., CA). The assay quality was verified by assessing no alteration of a set of housekeeping genes across the experimental parameters discussed in the supplementary section.
We have submitted the microarray data to the Gene Expression Omnibus (GEO) and this can be searched for using the GEO accession number: GSE54213.

A total of 28 biopsy specimens were collected from 2 red Duroc pigs and used in the evaluation of genome-wide transcriptome changes during the course of wound healing and compression application. Four biopsy specimens were collected from the left and right flanks of 2 pigs before wound creation and were used as a baseline of gene transcription. For assessment of wound progression and compression effects on scar during early, mid, and late wound-healing stages, 8 biopsy specimens were harvested on day 49, days 91-99, and days 119-133, respectively (4 per animal, 2 compression treated and 2 sham). Compression was applied between days 70 and 84 as indicated earlier. mRNA isolation is described in the “RNA and protein isolation” section.
Microarray assays were performed using Agilent-026440 S. scrofa (Pig) Oligo Microarray v2 expression array (GE 4x44K v2 2-color microarray) slides and kits (Agilent Technologies, Inc, Santa Clara, Calif) following the manufacturer's protocol. Hybridized microarray slides were scanned using Agilent Technologies Scanner G2505C US09493743.
Images of scanned microarray slides were feature-extracted and normalized using Agilent's feature extraction software, version 10.7 or later, in the default setup (Agilent Technologies, Inc).