Jagged2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Jagged2 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is a protein that functions as a ligand for the Notch family of receptors, which play a role in cell-cell communication and development.

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Lab products found in correlation

3 protocols using jagged2

Western Blot Analysis of Notch Signaling Proteins

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Proteins were extracted from WT and BACE1-null mice in RIPA buffer [50 mM Tris–HCl at pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1 mM NaF, 1 mM Na₃VO₄ and a protease inhibitor cocktail (Roche)]. Equal amounts of protein (50 µg) were resolved on a NuPAGE Bis-Tris Gel (Invitrogen, Palo Alto, CA) and transferred onto nitrocellulose membranes (Invitrogen) for western blot analysis. HRP-conjugated secondary antibodies were used and visualized using enhanced chemiluminescence (Thermo Scientific). Jagged1 (1:200), Jagged2 (1:200), and Delta1 (1:200) antibodies were purchased from Santa Cruz (Santa Cruz, CA). Notch1-ICD (1:1,000) was purchased from Cell Signaling (Boston, MA) and β-actin (1:10,000) was purchased from Sigma (St. Louis, MO).

Hu X., Hou H., Bastian C., He W., Qiu S., Ge Y., Yin X., Kidd G.J., Brunet S., Trapp B.D., Baltan S, & Yan R. (2017). BACE1 regulates the proliferation and cellular functions of Schwann cells. Glia, 65(5), 712-726.

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Antibody Expression Analysis of JAK-STAT Pathway

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Antibodies against JAK1, JAK2, JAK3, STAT1, STAT3, STAT6, p-STAT1, p-STAT3, p-STAT6, β-actin (Cell Signaling Technology, Beverly, MA, USA), Jagged1, Jagged2 and Notch1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for expression analysis. OVA, magnolol, methacholine and other chemicals and reagents used were got from Sigma-Aldrich (St. Louis, MO, USA) otherwise are specified.

Huang Q., Han L., Lv R, & Ling L. (2019). Magnolol exerts anti-asthmatic effects by regulating Janus kinase-signal transduction and activation of transcription and Notch signaling pathways and modulating Th1/Th2/Th17 cytokines in ovalbumin-sensitized asthmatic mice. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 23(4), 251-261.

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Investigation of GATA3, STAT6, and Notch Signaling

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Antibodies against GATA3, STAT6, p-STAT6, JAK1, p-JAK1 (Cell signalling Technology, USA), IL-4Rα, Jagged1, Jagged2, Notch1 intracellular domain (NICD1), Notch 2 intracellular domain (NICD2), Notch1, Notch 2 and GAPDH were purchased from Santa Cruz Biotechnology, USA. Methacholine, ovalbumin (OVA), punicalagin (98 %), and other reagents were acquired from Sigma-Aldrich (USA).

Yu L, & Li J. (2022). Punicalagin attenuated allergic airway inflammation via regulating IL4/IL-4Rα/STAT6 and Notch- GATA3 pathways. Acta pharmaceutica (Zagreb, Croatia), 72(4).

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