After 30 min of myocardial ischemia and 2 h after reperfusion, rats were anesthetized using an intraperitoneal injection of 1% sodium pentobarbital (3 mL·kg−1), and the left common carotid artery was detached. Carotid hemodynamics were recorded using the PowerLab 8-channel multifunctional physiological recorder (AD Instruments).
8 channel powerlab
Manufactured by ADInstruments
Sourced in United States
The 8-channel PowerLab is a data acquisition system that can record and analyze multiple physiological signals simultaneously. It features eight input channels, allowing for the concurrent measurement of various biological data.
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Lab products found in correlation
5 protocols using 8 channel powerlab
1
Carotid Hemodynamics in Myocardial Ischemia
2
Monitoring Cerebral Blood Flow in Infants
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All infants had measurement of ABP with an umbilical artery catheter placed for clinical monitoring. Middle cerebral artery CBFV was recorded with transcranial Doppler (Nicolet Vascular/Natus Medical Incorporated, San Carlos, CA). PaCO 2 was continuously recorded with a Neotrend-L fiber-optic sensor (Diametrics Medical, St Paul, MN). Analog data was collected simultaneously using a PowerLab 8 Channel data acquisition system (AD Instruments, Mountain View, CA). Digitized files were subsequently analyzed using ICM+ software (ICM+, Cambridge Enterprise, UK). Artifact was removed using valid values range filters and by visual inspection of each individual recording.
3
Cerebrovascular Hemodynamics Assessment
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The posterior cerebral artery (PCA) and middle cerebral artery (MCA) were insonated using two 2-MHz TCD probes (ST3; Spencer Technologies, Seattle, WA, USA) through the temporal acoustic window on the side of the head to record PCA and MCA velocity. The P-1 PCA and M-1 MCA segments were identified and the signals optimized corresponding to the depth of each vessel, velocity of blood flow and waveform produced (31 (link), 43 (link)). Participants were fitted with a three-lead electrocardiogram (ECG). Blood pressure (BP) was measured using finger photoplethysmography with a brachial cuff to adjust for height differences between the finger and brachial artery (Finometer PRO; Finapres Medical Systems, Amsterdam, Netherlands). End-tidal pressure of carbon dioxide (PETCO2) was sampled with a mouthpiece and monitored with an inline gas analyzer (ML206; ADInstruments, Colorado Springs, CO, USA), calibrated with a known gas concentration prior to each collection. All data were time-aligned and collected at a sampling frequency of 1,000 Hz via an 8-channel PowerLab (ADInstruments) and stored for offline analysis using commercially available software including LabChart (ADInstruments) and Microsoft Excel (Microsoft Corporation; Redmond, WA, USA).
4
Voluntary Exercise Monitoring in Rodent Heart Failure
5
Hypoxia-induced Cardiovascular Response
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Male C57BL/6 mice (the Jackson Laboratory), age between 12 to 14 weeks, were anesthetized by isoflurane (2.5%). Body temperature was maintained at 37°C by a heating pad connected to heat pump (Androit). Catheters were implanted for carotid artery and jugular vein. Arterial blood pressure was continuously recorded through a blood pressure transducer (MLT699, ADInstrument) connected to an 8-channel Powerlab (ADInstrument). A tracheal tube was introduced and connected to a rodent ventilator (Model 849, MidiVent). Upon completion, in some animals, the fraction of inspiratory O2 was decreased from 21% to 10% to induce hypoxia. 75μL of L-NAME was administered intravenously in a bolus at a dose of 10 mg/kg bodyweight. 10 minutes after L-NAME injection, animals were injected with 50 μL of NO-ferroheme or normal saline (NS) for 4 times at 10-minute intervals. An NO-ferroheme in albumin stock was prepared via a concentrated version of the direct method as described above: 300 μM ferric heme was mixed anaerobically on a Schlenk line with 3 mM GSH and 600 μM NO in 500 μM albumin using air-tight Hamilton syringes. This stock was diluted further in air with albumin solution before injection by 1000-, 100-, and 10-fold. Mean arterial pressure (MAP) values were obtained by processed the recorded arterial pressure data with LabChart 7.3.8 (ADInstruments).
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