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Matrigel coated 24 well transwell

Manufactured by Corning
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Sourced in United States

The Matrigel-coated 24-well Transwell is a laboratory equipment product designed for cell culture applications. It consists of a 24-well plate with permeable membrane inserts that allow for the study of cell migration, invasion, and co-culture experiments.

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2 protocols using matrigel coated 24 well transwell

1

Tube Formation Assay with Ovarian Cancer Exosomes

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Matrigel-coated 24-well transwell (8 μm pore size, Corning, New York, NY, USA) was used to perform tube formation assay. HUVECs were cultured into the matrigel-coated well at a density of 1×105 cells at 37°C with 5% CO2. Then, the cells were treated with ovarian cancer cells-derived exosomes with or without lncRNA siRNA2 for 24 h. Finally, a microscope was used to observe the results of tube formation.
Yuan D., Guo T., Zhu D., Ge H., Zhao Y., Huang A., Wang X., Cao X., He C., Qian H, & Yu H. (2022). Exosomal lncRNA ATB Derived from Ovarian Cancer Cells Promotes Angiogenesis via Regulating miR-204-3p/TGFβR2 Axis. Cancer Management and Research, 14, 327-337.
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2

Tube Formation Assay for HLECs

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The number of tube node formed in the HLECs was observed using a tube formation assay. Matrigel-coated 24-well Transwell® (8 μm pore size) was purchased from Corning, Inc. HLECs were cultured in CM or CM-TNF-α for 48 h. Next, HLECs (1 × 105 cells) were placed in the Matrigel®-coated well at 37°C. Next, the number of tube node of HLECs was observed using a microscope (IX51; Olympus) [32 (link)].
Chen X., Lin L., Wu Q., Li S., Wang H, & Sun Y. (2023). Tumor Necrosis Factor-α Promotes the Tumorigenesis, Lymphangiogenesis, and Lymphatic Metastasis in Cervical Cancer via Activating VEGFC-Mediated AKT and ERK Pathways. Mediators of Inflammation, 2023, 5679966.
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