Harvested cells were incubated with anti-D blood grouping reagent (human IgG/IgM monoclonal; Millipore, Billerica, MA, USA; dilution 1:10), antigen-presenting cell-conjugated anti-human-CD235a (BD Biocsciences, Franklin Lakes, NJ, USA; dilution 1:100) and phycoerythrin-conjugated anti-human IgG (Fc γ-specific; eBioscience, San Diego, CA, USA; dilution 1:100) in 100 μl of staining medium (PBS (Sigma) containing 2% FBS) for 20 min at room temperature. Stained cells were analysed using FACS Cantos II (BD Biosciences) and FlowJo (version X.0.7).
Facs cantos 2
Manufactured by BD
The FACS Cantos II is a flow cytometry instrument designed for cell analysis and sorting. It utilizes advanced optics and detection systems to provide high-performance data acquisition and cell sorting capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using facs cantos 2
1
Immunophenotyping of Blood Cell Populations
2
FACS Analysis of Transgenic Embryos
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FACS analysis was performed as described [38 (link)]. Embryos from globinLCR: GFP transgenic background were pooled. The cells were disaggregated and then sequentially filtered through 70 μm and 33 μm cell strainers. The percentage of GFP-positive cells in transgenic embryos was analyzed by FACSCantos II machine (BD Biosciences).
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