Chicken HD11 and DF-1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Cellmax, Beijing, China) supplemented with 10% fetal bovine serum (FBS, Cellmax) and 100 IU/mL penicillin–streptomycin solution (Cellmax). All cells were cultured in an incubator at 37 °C in 5% CO2.
The IBV Beaudette strain (GenBank: DQ001339) was kindly gifted by Prof. Ding-Xiang Liu, South China Agricultural University.
Mouse monoclonal antibodies specific for Flag and β-actin, rabbit polyclonal anti-Myc antibodies, CoraLite488-conjugated donkey anti-mouse IgG (H + L), CoraLite594-conjugated donkey anti-rabbit IgG (H + L), HRP-conjugated Affinipure donkey anti-mouse IgG (H + L), and HRP-conjugated Affinipure donkey anti-rabbit IgG (H + L) were purchased from Proteintech (Wuhan, China). Mouse monoclonal anti-IBV N protein antibodies were from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-chJAK1 and anti-chSTAT1 antibodies were from Abmart (Shanghai, China) and Bioss (Beijing, China), respectively.
The IBV Beaudette strain (GenBank: DQ001339) was kindly gifted by Prof. Ding-Xiang Liu, South China Agricultural University.
Mouse monoclonal antibodies specific for Flag and β-actin, rabbit polyclonal anti-Myc antibodies, CoraLite488-conjugated donkey anti-mouse IgG (H + L), CoraLite594-conjugated donkey anti-rabbit IgG (H + L), HRP-conjugated Affinipure donkey anti-mouse IgG (H + L), and HRP-conjugated Affinipure donkey anti-rabbit IgG (H + L) were purchased from Proteintech (Wuhan, China). Mouse monoclonal anti-IBV N protein antibodies were from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-chJAK1 and anti-chSTAT1 antibodies were from Abmart (Shanghai, China) and Bioss (Beijing, China), respectively.