The largest database of trusted experimental protocols

2 protocols using 2 cyclohexylamino ethane sulfonic acid

1

Synthesis and Characterization of CEHC

Check if the same lab product or an alternative is used in the 5 most similar protocols
(Z)-2-(1-(3-oxo-3H-Benzo[f]chromen-2-yl)ethylidene)hydrazine-1-carbothioamide (CEHC) was purchased from Analytics Co. (https://www.analytics-shop.com). Its chemical structure is illustrated in Scheme 1. All inorganic salts were of analytical grade and obtained from Sigma-Aldrich (https://www.sigmaaldrich.com). All the chemicals were of analytical grade and were not purified prior to use. ChloroAcetic acid and sodium chloroacetate were obtained from Merck. Acetic acid and sodium acetate were obtained from Sigma-Aldrich. Piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), and 2-(cyclohexylamino) ethane sulfonic acid (CHES) were obtained from Sigma-Aldrich Co.
+ Open protocol
+ Expand
2

Preparation of High-Purity Aqueous Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
N-[tris(hydroxymethyl)methyl]glycine (Tricine, purity > 99 wt%), 2-[(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethane sulfonic acid (TES, purity > 99 wt%), 2-(N-morpholino) ethanesulfonic acid (MES, purity > 99 wt%), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES, purity > 99.5 wt%), 2-(cyclohexylamino)ethanesulfonic acid (CHES, purity > 99 wt%), and [P4444][OH] (45 wt% in water), were purchased from Sigma–Aldrich. Sodium hydroxide pellets were supplied from Eka Chemicals. BSA (fraction V) was obtained from Acros Organics. Methanol (HPLC grade, purity > 99.9 wt%) was obtained from Fisher Scientific (UK). Acetonitrile (purity > 99.7 wt%) was supplied from Lab-Scan (Ireland). Purified water passed through a reverse osmosis and a Milli-Q plus 185 water purifying system was used in all experiments. Sodium nitrate (purity > 99.5 wt%) was obtained from Himedia Labs. Potassium nitrate (purity > 98.0), nitric acid (65 wt%), and potassium hydrogen phthalate (purity > 99.8) were purchased from Panreac (Barcelona, Spain).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!