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Rab 7 is a small GTPase protein that plays a key role in the regulation of endocytic and lysosomal trafficking pathways. It is involved in the formation, transport, and fusion of late endosomes and lysosomes, which are essential cellular organelles responsible for the degradation and recycling of macromolecules. Rab 7 serves as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state, to coordinate these trafficking events.

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Lab products found in correlation

V5 tag, by Thermo Fisher Scientific (1 mentions) Transferrin receptor tfr, by Abcam (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) Lamp1, by Developmental Studies Hybridoma Bank (1 mentions) A8717, by Merck Group (1 mentions) Ab109390, by Abcam (1 mentions) Ab5622, by Thermo Fisher Scientific (1 mentions) Bace1, by Cell Signaling Technology (1 mentions)

2 protocols using rab 7

1

Antibody Characterization for Cell Biology

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Antibodies were purchased from the following commercial sources: EGFR C-terminal (Invitrogen; Cat # PA1–1110; 1:200), EGFR N-terminal (E234; Abcam; Cat # ab32198; 1:1000), V5-tag (Invitrogen; Cat # 46–0705; 1:5000), c-Myc (9E10, Santa Cruz Biotechnologies; Cat # sc-50; 1:500), EEA1 (Thermo Fisher; Cat # PA5–17228; 1:500), LAMP1 (H4A3; Developmental Studies Hybridoma Bank; Cat # H4A3; 1:500), Rab11 (Millipore; Cat # 2413S; 1:1000), Rab 7 (Invitrogen; Cat #D95F2; 1:1000), Transferrin Receptor (TfR, Abcam; Cat # ab190640; 1:1000).
Gireud-Goss M., Reyes S., Wilson M., Farley M., Memarzadeh K., Srinivasan S., Sirisaengtaksin N., Yamashita S., Waxham M.N., Tsunoda S., Lang F.F, & Bean A.J. (2018). Distinct mechanisms enable inward or outward budding from late endosomes/multivesicular bodies. Experimental cell research, 372(1), 1-15.
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2

Antibody Sources for Neuronal Markers

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Antibodies were purchased from the following commercial sources: C-terminal APP (Sigma; A8717; 5 μg/mL for immunoblotting (IB); 50 μg/mL for immunocytochemistry (ICC), N-terminal APP (Millipore; 22C11; 10 μg/mL for ICC), MAP2 (Millipore, AB5622; 5 μg/mL); Rab7 (Invitrogen; # D95F2; 2.5 μg/μL for ICC, 0.25 μg/mL for IB), EEA1 (Thermo Fisher; Cat. # PA5–17228; 2.5 μg/μL for ICC, 0.25 μg/mL for IB), BACE-1 (Cell Signaling; D10E5; 1 μg/mL), Presenilin 2 (Abcam; ab51249; l:1000),quan Actin (Sigma; A2066; 0.3 μg/mL), UBE4B (Abcam; ab126759; 0.3 μg/mL for IB; ab97697), Ubiquitin (Enzo; P4D1; 1 μg/μL), Hrs (Axxora; A-5; 1 μg/μL), EGFR (Invitrogen; Cat # PA1–1110; 1:200), phospho Tau (Abcam; ab109390; 1:1000).
Gireud-Goss M., Reyes S., Tewari R., Patrizz A., Howe M.D., Kofler J., Waxham M.N., McCullough L.D, & Bean A.J. (2020). The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion. Molecular and cellular neurosciences, 108, 103542.
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