Coumarin and 4-Hydroxycoumarin were obtained from Acros organics, 3-Hydroxycoumarin, 3-(2-Hydroxyphenyl) propionic acid, and 7-hydroxycoumarin (7OHC) were obtained from Sigma-Aldrich, trans-o-Coumaric acid was obtained from Tokyo chemical Industry Co., and 6-Hydroxycoumarin was obtained from Fisher scientific. All chemicals had purities of 98 % or higher. Methanol (≥99.8 %) and acetic acid (≥99.8 %) were procured from Fisher Scientific and Sigma Aldrich, respectively. All chemicals were used as received. Stock solutions of coumarin (8.2 mM) and its products (100 μM) were prepared to their desired concentration and diluted using distilled water when required. Calibration curves for each chemical were prepared using their associated stock solution and analysed using high performance liquid chromatography (HPLC). HPLC mobile phases were prepared using deionised water. The initial concentration of coumarin for this study was 100 µM (15 ppm), as followed in our previous study pertaining to dosimetry [26] .
3 2 hydroxyphenyl propionic acid
Manufactured by Merck Group
Sourced in Australia
3-(2-Hydroxyphenyl) propionic acid is a chemical compound with the molecular formula C9H10O3. It is a carboxylic acid that contains a hydroxyl group and a phenyl group. The compound is used as a reagent in various laboratory and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
4 hydroxycoumarin, by Thermo Fisher Scientific (1 mentions)
3 hydroxycoumarin, by Merck Group (1 mentions)
Lc ms grade formic acid, by Merck Group (1 mentions)
Porcine pepsin, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Yeast extract, by Merck Group (1 mentions)
Mucin, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
Pectin, by Merck Group (1 mentions)
Pancreatin, by Thermo Fisher Scientific (1 mentions)
Casein, by Merck Group (1 mentions)
Pyrogallol, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Chem-Supply (1 mentions)
Mrs agar, by Merck Group (1 mentions)
4 methylvaleric acid, by Merck Group (1 mentions)
Sodium chloride (nacl), by Chem-Supply (1 mentions)
Peptone, by Merck Group (1 mentions)
Potato starch, by Merck Group (1 mentions)
2 protocols using 3 2 hydroxyphenyl propionic acid
1
Quantitative Analysis of Coumarin and Its Metabolites
2
Yoghurt Starter Culture and Probiotics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A commercial yoghurt starter culture (YOFLEX®—consisting of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and three probiotic strains (Lacticaseibacillus rhamnosus LGG, Lacticaseibacillus casei 431, Bifidobacterium animalis subsp. lactis BB-12) were kindly supplied by Chr. Hansen, Bayswater, VIC, Australia. Powdered skim milk (Australian instant skim milk powder, Coles) was obtained from a local supermarket in Melbourne, VIC, Australia. Pancreatin was purchased from Alfa Aesar (Ward Hill, MA, USA). Mangiferin, pyrogallol, gallic acid, caffeic acid, 3-hydroxy phenyl acetic acid, 3-(2-hydroxyphenyl) propionic acid, porcine pepsin, α-amylase from aspergillus oryzae, p-nitrophenyl-α-d-glucopyranoside, bile salts, LC-MS grade formic acid, acetic acid, 4-methyl valeric acid, guar, casein, tryptone, cysteine HCl, peptone, mucin, pectin, potato starch, yeast extract, and MRS agar were acquired from Sigma–Aldrich (Castle Hill, NSW, Australia). Sodium carbonate (anhydrous), HCl, NaHCO3, NaOH, (NH4)2CO3, KCl, NaCl, KH2PO4, MgCl2(H2O)6, CaCl2, K2HPO4, Na3PO4, MgSO4.7H2O Tween 80, orthophosphoric acid, acetonitrile, and methanol of HPLC grade were acquired from Chem-supply Pty Ltd. (Melbourne, VIC, Australia).
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