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Pb 10 ph meter

Manufactured by Sartorius
Sourced in Germany, China

The PB-10 pH meter is a compact and accurate instrument designed for measuring the pH of liquid samples. It features a digital display, automatic temperature compensation, and a simple one-button calibration process. The PB-10 provides reliable pH measurements in a variety of laboratory and industrial applications.

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54 protocols using pb 10 ph meter

1

Advanced Material Characterization Techniques

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TEM and HRTEM images were taken with
a JEM-2100F high-resolution transmission emission microscope (JEOL,
Japan). XRD spectrum was measured on a Bruker D-8 Advance Powder X-ray
diffractometer (Bruker, Germany). XPS spectra were taken with ESCALAB
250Xi X-ray photoelectron spectroscopy (Thermo Fisher Scientific).
FT-IR spectrum was recorded on a Thermo Nicolet iS10 spectrometer
(Thermo Fisher Scientific). Elemental analysis was carried out on
a Vario EL/Micro Cube organic element analyzer (Elementar Analysensysteme
GmbH, Germany). Magnetic property was recorded by using a VersaLab
Vibration Sample Magnetometer (Quantum Design). UV–vis absorption
spectra were recorded on a Shimadzu UV-3600 Plus UV–vis–NIR
spectrophotometer (Shimadzu, Japan). Fluorescence spectra were recorded
on a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher
Scientific). Time-resolved fluorescence spectra were measured on a
Horiba Scientific QM-8075 high sensitivity steady-state transient
fluorescence spectrometer (HORIBA, Japan). ζ-Potential was recorded
on a Zetasizer Nano ZS (Malvern, U.K.). CV curves were obtained from
a Chenhua CHI-760E electrochemical workstation (Shanghai, China).
The pH values were mediated using a Sartorius PB-10 pH meter (Sartorius,
China).
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2

pH Monitoring During Fermentation

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pH variations during alcoholic and acetic acid fermentation were detected by Sartorius PB-10 pH meter (Sartorius, Göttingen, Germany) [35 (link)].
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3

Analytical Techniques for Biomolecular Studies

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The main instruments used in this study were described as below: Sartorius PB-10 pH meter (Sartorius, Germany); Evolution 200 Ultraviolet–Visible Spectrophotometer (Thermo Fisher Scientific, USA); BT25S Electronic Balance (Sartorius, Germany, d = 0.01 mg); Ultrapure Water Machine (Millipore, USA); Bio-Rad ChemiDoc XRS+ System with Image Lab software and Bio-Rad Mini-Protean® Tetra system; DMI3000B Inverted Research Grade Microscope with Leica Application Suite V4.4.0 (Leica, Germany); 5427R Microcentrifuge (Eppendorf, Germany); Triple TOF 5600 Mass Spectrometer fitted with Eksigent NanoLC-Ultra 2D system, Nanospray III source, and Protein Pilot 5.0 software (AB SCIEX, MA, USA); and high-performance liquid chromatography (HPLC) system (Agilent 1200, USA).
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4

Tomato Juice Supplement Composition Optimization

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TJSM was prepared according to the method described previously [20 (link)]. Briefly, tomatoes (Solanum lycopersicum) purchased from local market were cut into small cubes, ground in a pulper and filtered through cotton gauze to remove the majority of the peel and seeds. The filtrated tomato juice was further centrifuged to remove the fruit debris. The major biochemical components of the tomato juice were determined in triplicate and listed in Table 1. No obvious difference in the components was determined among different variety of tomato (see Supplementary material, Figure S1, Table S1). The tomato juice was supplemented with sucrose at 50, 100, 150, 200 or 250 g/L, and the pH value of the mixture was adjusted to 4.5, 5.5, 6.5, 7.5, or 8.5 individually by addition of 5.0 M NaOH. The TJSM was sterilized at 121 °C for 20 min.

Major biochemical components and parameters of tomato juice (values are the average ± range of triplicate (3) analyses)

Component/parameterLevel
Density1.018 ± 0.002 g/100 mL
Ash0.42 ± 0.01 g/100 mL
Free reducing sugarsa2.67 ± 0.03 g/100 mL
Total Kjeldahl nitrogenb0.074 ± 0.001 g/100 mL
Fatc0.10 ± 0.01 g/100 mL
pHd4.15 ± 0.03

a Determined by the DNS method[52], using glucose as the standard

b Determined using a Tecator Kjeltec 1025 system

c Determined using a Tecator Soxtec HT2 system

dDetermined using a Sartorius PB − 10 pH meter

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5

Soil Moisture and Organic Carbon Analysis

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Each soil sample was placed in a plastic Ziplock bag with its cutting ring and weighed (g) on site. In the laboratory, each sample was removed from the plastic bag and placed in an individual pre-weighed aluminum box, then dried in an oven at 105 • C for 24 h. Each soil sample was again weighed, and its net dry weight calculated as the total weight minus that of the aluminum box. The SWC was then calculated as [35] (link)
where W 1 = fresh weight, and W 2 = dry weight. SBD was then calculated as SBD (Mg m -3 ) [37] = dry weight volume . The pH of a 2:5 soil: water suspension was measured using a Sartorius PB-10 pH meter [37] (link), and the SOC (g kg -1 ) was determined with wet dichromate oxidation using an air-dried homogenized subsample of 0.2 g soil and titration with FeSO 4 [38] (link).
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6

Soil Chemical Properties Characterization

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Soil pH value was determined in water suspension [1: 2.5 (w/ v) soil/distilled water ratio] by a Sartorius PB-10 pH meter. Exchangeable acid quantum and hydrogen were measured according to KCl exchange-neutralization titration method (SSIR 2004) . Cation exchange capacity (CEC) was determined by the method of ammonium acetate saturation (CH3COONH4, 1 mol L -1 , pH 7.0). Soil was digested by hot HF, HNO3, HClO4, and H2O2 mixture and analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) (Varian 710-ES) in order to determine total Al concentration (Kubová et al. 2005) . The optimized six-step sequential extraction scheme was applied according to the method of Larssen et al. (1999) , and Al concentrations in the extracts were also determined by ICP-OES (Varian 710-ES) (Table 2). Soil-exchangeable Ca, Mg, K, and Na were extract-ed with 1 mol L -1 CH3COONH4 (pH 7.0) and then measured by atomic absorption spectrometry as previously described (Moro et al. 2014) . All subsequent data were expressed on dry weight bases.
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7

Comprehensive Food Characterization Protocol

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The pH value was recorded with a PB-10 pH meter (Sartorius, Beijing, China). The WHC was analyzed via centrifugation at 4000× g for 10 min according to Li et al. [15 (link)]. The supernatant was immediately discarded and WHC (%) was calculated as the ratio of the residual pellet weight to the original weight before centrifugation. Viscosity was measured with a HAAKE MARS 40 rotational rheometer (Thermo Fisher Scientific Inc., Harbin, China) using a parallel plate (diameter 35 mm, gap 0.05 mm). The apparent viscosity of plain and mixed samples was determined at 25 °C in the shear rate ( γ˙ ) range from 0.1 to 100 s−1 [16 (link)].
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8

Grape Juice Analysis: SSC, pH, TA

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Grape juice was obtained using a food blender after the seeds were removed. The juice was then filtered through four layers of cheesecloth in preparation for measurement of total SSC and pH. Total SSC was measured by a hand-held refractometer (RHB-18ATC, Shanghai, China) at 20 °C and the appropriate temperature correction adjustments were applied, and the results were expressed as %. The pH was determined by using a PB-10 pH meter (Sartorius, Göttingen, Germany). TA was determined by potentiometric titration with 0.1 N NaOH up to pH = 8.2 and expressed in % of tartaric acid [31 (link)]. All experiments were performed in quadruplicate.
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9

Aerobic Treatment of Agro-Industrial Waste

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All experiments were carried out under the aerobic condition at 30 °C with constant shaking (150 rpm). Microbial growth was monitored indirectly by measuring the absorbance at 600 nm (Buck Vis 100 Spectrophotometer). The color (ADMI unit), COD (reactor digestion method), and ammoniacal nitrogen (Nessler method) were determined by HACH DR 5000 (HACH 2005 ). The pH was measured using Sartorius PB-10 pH meter. Total polyphenolic compounds were quantified with Folin–Ciocalteau Reagent using gallic acid as the standard. The lignin content of the samples was estimated using KL as the standard (Neoh et al. 2013b (link)). All experiments were conducted in triplicates.
Inductively coupled plasma-mass spectrometry (ICP-MS, Perkin Elmer Elan 6100) was applied for determination of the heavy metals in this work. The ICP-MS was operated using argon gas as carrier gas with gas flow of 0.435 l/min. AnPOME before and after treatment were filtered using 0.2 µm membrane and acidified to pH 2 with HNO3 for metal analysis.
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10

Homogenizing Pork Samples for pH Analysis

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A pork sample (10 g) was placed in a sterile homogenizing bag, distilled water (100 mL) added, then homogenized (Scientz-09, Scientz, Ningbo, Zhejiang) at 10,000 rpm for 1 min. The pH of the homogenate was determined with a PB-10 pH meter (Sartorius, Beijing, China).
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