A375 melanoma

CT26 colon carcinoma (CRL‐2638), A375 melanoma (CRL‐1619), and B16‐F10 (CRL‐6475) melanoma were obtained from American Type Culture Collection (ATCC). 293FT cells were purchased from Invitrogen (Carlsbad, CA). B16‐OVA cells were generated using Lipofectamine 3000 (Invitrogen, Waltham, MA) with a cDNA encoding ovalbumin (OVA). Single colonies were isolated, expanded, and characterized for OVA expression. In order to generate A375 cells stably expressing a firefly luciferase (A375‐LUC), A375 cells were transfected with a plasmid pSBbi‐BP (Addgene plasmid #60512) expressing firefly luciferase gene and selected using puromycin.⁴⁵ All tumor cell lines and genetically modified lines derived from them were cultured in RPMI‐1640 or DMEM medium with 10% heated‐inactivated FBS and 1× penicillin/streptomycin/glutamine (Thermo Fisher Scientific, San Francisco, CA). All cell lines were tested for mycoplasma contamination by qPCR analysis (BioMax, South Korea).⁴⁶

The human melanoma cell line A375 melanoma was purchased from the American Type Culture Collection (Rockville, MA, USA, CRL-1619). A375 cells were cultivated at 37 °C in a humidified incubator supplied with 5% CO₂. The medium consisted of DMEM supplemented with 10% of fetal bovine serum, penicillin (100 U ml⁻¹) and streptomycin (100 μg ml⁻¹). When the A375 cells were 80% confluent, they were treated with bacterial LPS (10 μg ml⁻¹). Medium and other cell culture reagents were obtained from Gibco-BRL (Grand Island, NE, USA). Precast IPG strips and other reagents used in 2-DE experiments were from Amersham Biosciences (Uppsala, Sweden). Antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

The erythroleukemia cell line K562 and the mouse melanoma cell line B16 were purchased from the Japanese Collection of Research Bioresources cell bank (Osaka, Japan). The CD19⁺ B-cell leukemia cell line NALM6 and the mouse colon carcinoma cell line Colon-26 were obtained from the Cell Resource Center for Biomedical Research, Tohoku University (Sendai, Japan). The A375 melanoma, PG13 retroviral packaging cell line, and AsPC1 pancreatic cancer cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). The Plat-A and Plat-E packaging cell lines were kindly provided by Dr. T. Kitamura (University of Tokyo, Tokyo, Japan). K562, NALM6, Colon 26, AsPC1, and their derivatives were cultured in RPMI-1640 (Nacalai Tesque, Japan) containing 10% fetal bovine serum (FBS) (Nichirei Biosciences, Tokyo, Japan). A375 and PG13 cells were cultured in DMEM (Nacalai Tesque, Kyoto, Japan) containing 10% FBS. B16 cells were cultured in MEMα (Nacalai Tesque) supplemented with 10% FBS. A375 cells were transduced with CD19 and mesothelin to generate A375-CD19 and A375-mesothelin, respectively.