Act diff
Manufactured by Beckman Coulter
Sourced in United States
The AcT/Diff is a hematology analyzer that provides complete blood count (CBC) and white blood cell differential results. It measures key parameters such as red blood cells, white blood cells, and platelets, delivering accurate and reliable data to support clinical decision-making.
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20 protocols using act diff
1
Plasma Hemoglobin Determination Using Beckman Coulter Analyzer
2
White Blood Cell Enumeration from Peripheral Blood
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White blood cells from fresh peripheral blood samples were counted in the hematological counter (AcT diff, Beckman Coulter, Brea, CA, USA). 100 uL or up to 1 × 106 cells were added to 12 × 75 mm tubes and stained with the extracellular antibodies for 15 min, in the dark at RT. After incubation red blood cells were lysed with BD Lysing Solution (Becton Dickinson, San Jose, CA, USA), for 10 min. Cell suspensions were centrifuged and washed one time with 1 × PBS in same conditions. In the end, cell suspensions were acquired in FACS Canto II with DIVA software (Becton Dickinson, San Jose, CA, USA). The antibodies used for cellular staining are described in Supplementary File 2 .
3
Xenograft Model for Evaluating Anti-cancer Drugs
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HCC827-GR cells were injected subcutaneously to generate a xenograft model in
mice. Then the mice were randomly divided into 4 groups (n = 6): the normal
group (non-treatment), the negative control group (10 mL/kg saline), the
afatinib group (10 mg/kg), the HAD-B1 group (400 mg/kg), and the combined group
(afatinib with 10 mg/kg plus HAD-B1 with 400 mg/kg). Once the tumor size had
reached 100 mm3 (link), HAD-B1 and afatinib were administered orally once daily for
4 weeks, during which time the tumor size and the body weight were checked
daily. Hematology and blood chemistry were performed using a hematology analyzer
(Ac. T diff™, BECKMAN COULTER) and a biochemical analyzer (A7020, Hitachi). All
care and handling of the animals were performed according to the Guide for the
Care and Use of Laboratory Animals (HTRC-16-37(1)).
mice. Then the mice were randomly divided into 4 groups (n = 6): the normal
group (non-treatment), the negative control group (10 mL/kg saline), the
afatinib group (10 mg/kg), the HAD-B1 group (400 mg/kg), and the combined group
(afatinib with 10 mg/kg plus HAD-B1 with 400 mg/kg). Once the tumor size had
reached 100 mm3 (link), HAD-B1 and afatinib were administered orally once daily for
4 weeks, during which time the tumor size and the body weight were checked
daily. Hematology and blood chemistry were performed using a hematology analyzer
(Ac. T diff™, BECKMAN COULTER) and a biochemical analyzer (A7020, Hitachi). All
care and handling of the animals were performed according to the Guide for the
Care and Use of Laboratory Animals (HTRC-16-37(1)).
4
Malaria Parasite Examination and Treatment
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Children were examined and passively followed for febrile illnesses at the parents’ request by a medical doctor in the medical unit established at the Makélékélé district hospital, located about 6 km from the study area. Fingerprick capillary blood was obtained to prepare Giemsa-stained smears for malaria parasite examination by microscopy. After obtaining informed consent from one of the parents, venous blood sample was collected from children with positive smears for P. falciparum malaria parasites in ethylene diaminetetraacetic acid (EDTA)-coated tubes to determine haematological parameters using Beckman Coulter ACT Diff (Beckman Coulter; Brea, CA, USA).
Febrile children with positive smears were randomized to receive ASAQ or AL if the following conditions were met: monoinfection with P. falciparum, parasitaemia ≥1000 asexual parasites per microlitre of blood, absence of severe malnutrition, danger signs (i.e., inability to stand, breastfeed, or drink, recent convulsions, lethargy, or persistent vomiting), signs of severe and complicated malaria, and any febrile conditions due to diseases other than malaria [25 ].
Febrile children with positive smears were randomized to receive ASAQ or AL if the following conditions were met: monoinfection with P. falciparum, parasitaemia ≥1000 asexual parasites per microlitre of blood, absence of severe malnutrition, danger signs (i.e., inability to stand, breastfeed, or drink, recent convulsions, lethargy, or persistent vomiting), signs of severe and complicated malaria, and any febrile conditions due to diseases other than malaria [25 ].
5
PBMC Isolation and Characterization
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Peripheral blood mononuclear cells (PBMCs) were isolated from 8 ml of whole blood by a CPT tube system (BD Sciences). The separated plasma was aliquoted and stored at -20°C. The extracted PBMCs were frozen in cell-freezing medium (Gibco) and stored in a deep freezer (-80°C). After thawing the samples, the number of total cells was counted (Beckman Coulter AcT/Diff), and the relative proportions of cell populations were determined by flow cytometry using surface markers. Subsets of T cells (CD3+), B cells (CD19+), NK cells (CD16+ CD56+), and monocytes (CD14+) were identified. In addition, cell viability was checked, and the percentage of dead cells was determined.
6
Comprehensive PBMC Immunophenotyping Protocol
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The frozen PBMC samples were thawed, the number of total cells was counted (Beckman Coulter AcT/Diff). Next, relative fractions of T cells (CD3+/CD4+ & CD3+/CD8+), B cells (CD19+/CD20+), monocytes (CD14+) and NK cells (CD16+/CD56+) were determined in freshly thawed and unstimulated PBMCs via flow cytometry using standard protocols for FACS surface staining. Briefly, cells were stained with following antibodies using two panels: CD3‐Alexa Fluor 488 (clone UCHT1), CD4‐PerCP‐Cy5.5 (clone RPA‐T4), CD8‐APC‐H7 (clone SK1), CD19‐PE‐Cy7 (clone SJ25C1), CD20‐Alexa Fluor 700 (2H7), CD16‐PE (clone 3G8), CD56‐PE (clone MY31) and CD14‐APC (clone M5E2, all antibodies were purchased from BD Pharmingen). Additionally, both panels were stained with CD45‐PE‐TexasRed (clone HI30, Invitrogen). In addition, cell viability was checked and percentage of dead cells was determined. Cells were analysed using a BD FACS Canto II flow cytometer and data was analysed using the FlowJo software (BD Biosciences).
7
Automated Hematocrit Assessment Methods
8
Whole Blood Complete Blood Counts
9
Blood Analysis After Total-Body Irradiation
10
Comprehensive Blood Parameter Analysis
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EDTA-treated blood was analyzed using a laser-based Beckman Coulter Ac-T diff hematology analyzer to determine total white blood cell counts, white blood cell differentials, red blood cell counts, platelet counts, hematocrit values, total hemoglobin concentrations, mean cell volumes, mean corpuscular volumes, and mean corpuscular hemoglobin concentrations. A Piccolo point-of-care analyzer and biochemistry panel plus analyzer discs (Abaxis) were used to test for serum concentrations of albumin, amylase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), glucose, cholesterol, total protein, blood urea nitrogen (BUN), creatinine (CRE), uric acid, and C-reactive protein (CRP).
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