Apc anti human foxp3 antibody kit

Manufactured by Thermo Fisher Scientific

The APC anti-human Foxp3 antibody kit is a laboratory reagent used for the detection and analysis of the Foxp3 transcription factor in human cells. Foxp3 is a key regulatory protein involved in the development and function of regulatory T cells. This kit provides the necessary antibody and reagents to identify and quantify Foxp3-expressing cells through flow cytometry or other immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Leukocyte activation cocktail with goldiplug, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Hla dr v500, by BD (1 mentions) Cd127 pe cy7, by BioLegend (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Anti cd25 apc cy7, by BioLegend (1 mentions) V500 anti hla dr, by BD (1 mentions) Anti cd127 pe cy7, by BioLegend (1 mentions) Pe anti il 4, by BioLegend (1 mentions) Anti ifn γ apc, by BioLegend (1 mentions) Cytofix cytoperm fixation permeabilization solution kit, by BD (1 mentions) Anti cd8, by BioLegend (1 mentions) Anti cd4, by BD (1 mentions) Anti cd11c fitc, by BD (1 mentions)

Spelling variants of this product

Anti-Foxp3 (119 citations) Foxp3-APC (77 citations) Anti-Foxp3-APC (68 citations) Anti-Foxp3 antibody (25 citations) Foxp3 antibody (13 citations) Anti-human FOXP3-APC (8 citations) APC anti-FOXP3 antibody (2 citations) Anti-human FoxP3 antibody (2 citations)

2 protocols using apc anti human foxp3 antibody kit

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For surface staining, cells were incubated with antibodies diluted in PBS containing 2% FBS for 20 min, on ice. Conjugated antibodies to CD11c (FITC), CD124 (PE), CD14 (PERCP), CD80 (PE-Cy7), CD83 (APC), CD40 (APC-Cy7), CD86 (pacific blue) and HLA-DR (V500) were from BD Biosciences. Conjugated antibodies to CD4 (APC, APC-Cy7 and Alexa-Fluor 488), CD8 (PE and PERCP), CD25 (APC-Cy7) and CD127 (PE-Cy7) were from Biolegend. Intracellular cytokine staining was performed with BD Cytofix/Cytoperm kit (BD Biosciences). Cells were stimulated with Leukocyte Activation Cocktail with GoldiPlug (BD Biosciences) for 5 hours at 37°C in 5% CO₂, and were then stained with PE anti-IL-4 and APC anti-IFNγ (Biolegend). Regulatory T cells (CD4⁺CD127⁻CD25⁺Foxp3⁺) were stained for intracellularFoxp3 using the APC anti-human Foxp3 antibody kit (eBioscience). Flow cytometry was performed using a FACSCanto II (Becton Dickinson, Franklin Lakes, NJ), and theresults were analyzed with FlowJo 8.7 software.

Toniolo P.A., Liu S., Yeh J.E., Ye D.Q., Barbuto J.A, & Frank D.A. (2016). Deregulation of SOCS5 suppresses dendritic cell function in chronic lymphocytic leukemia. Oncotarget, 7(29), 46301-46314.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Immune Cell Phenotypes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were stained on ice in PBS containing 2% FBS for 20 min, using the following mAbs: FITC anti-CD11c; PERCP anti-CD14; PE-Cy7 anti-CD80; APC anti-CD83; APC-Cy7 anti-CD40; pacific blue anti-CD86; and V500 anti-HLA-DR (BD Biosciences); anti-CD4 (APC, APC-Cy7 and Alexa-Fluor 488); anti-CD8 (PE and PERCP); APC-Cy7 anti-CD25; and PE-Cy7 anti-CD127 (Biolegend). For intracellular cytokine staining, cells were stimulated with Leukocyte Activation Cocktail with GoldiPlug (BD Biosciences) for 5 hours at 37°C in 5% CO₂. Cells were permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization solution kit (BD Biosciences). Intracellular staining was performed with PE anti-IL-4, APC anti-IFNγ, PE-Cy7 anti-IL-10 and FITC anti-TNF (Biolegend). To quantitate Tregs (CD4⁺CD127⁻CD25⁺Foxp3⁺), cells were stained for intracellular Foxp3 using the APC anti-human Foxp3 antibody kit (eBioscience). Flow cytometry was performed using a FACSCanto II (Becton Dickinson, Franklin Lakes, NJ), and the results were analyzed with FlowJo 8.7 software.

Toniolo P.A., Liu S., Yeh J.E., Moraes-Vieira P.M., Walker S.R., Vafaizadeh V., Barbuto J.A, & Frank D.A. (2015). Inhibiting STAT5 by the BET bromodomain inhibitor JQ1 disrupts human dendritic cell maturation. Journal of immunology (Baltimore, Md. : 1950), 194(7), 3180-3190.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!