Cms chip

Example 4

A surface plasmon resonance (SPR) experiment was progressed using a biosensor chip to compare binding forces for acetylcholine receptors of S6_1 (SEQ ID NO: 23, WTWKGKGTLNR), i.e., discovered peptides and S6_1_C6 (SEQ ID NO: 31, KGTLNR), i.e., a deleted form, and Synake and Vialox, i.e., a positive control group (Biacore 3000, Biacore AB, Uppsala, Sweden).

After fixing selected acetylcholine receptor proteins to a CMS chip (Biacore) using EDC/NHS, association and dissociation were observed for up to 500 seconds. A binding force comparing experiment was carried out under observation conditions of a running buffer of 20 mM Tris (pH 7.4), a speed of 30 [Figure (not displayed)]/min, and a peptide concentration of 10 μM (Synake, Vialox, S6_1, S6_1_C6). Results of the binding force comparing experiment are shown in FIG. 6.

Example 3

S6_1 (SEQ ID NO:23, WTWKGKGTLNR), S6_2 (SEQ ID NO: 2 4, WTWKGRKSLLR), S6_3 (SEQ ID NO: 25, WTWKGEDKGKN), S6_4 (SEQ ID NO: 26, WTWKGRDKLQM) showing sequence similarities through multiple alignments among the peptides in Table 2 were synthesized.

A surface plasmon resonance (SPR) experiment was progressed using a biosensor chip to compare binding forces for the acetylcholine receptors thereof (Biacore 3000, Biacore AB, Uppsala, Sweden). After fixing selected acetylcholine receptor proteins to a CMS chip (Biacore) using EDC/NHS, association and dissociation were observed for up to 500 seconds. A binding force comparing experiment was carried out under observation conditions of a running buffer of 20 mM Tris (pH 7.4), a speed of 30 [Figure (not displayed)]/min, and a peptide concentration of 10 μM (S6_1, S6_2, S6_3, S6_4). Results of the binding force comparing experiment are shown in FIG. 5.

Example 5

A surface plasmon resonance (SPR) experiment was progressed using a biosensor chip to check affinity values for acetylcholine receptors of S6_1 (SEQ ID NO: 23, WTWKGKGTLNR), i.e., discovered peptides and Synake, i.e., a positive control group (Biacore 3000, Biacore AB, Uppsala, Sweden). After fixing the acetylcholine receptors to a CMS chip (Biacore) using EDC/NHS, association and dissociation were observed for up to 500 seconds. A binding ability comparing experiment was carried out under observation conditions of a running buffer of 20 mM Tris (pH 7.4), a speed of 30 [Figure (not displayed)]/min, a concentration of 10 to 50 μM (Synake), and a concentration of 0.1 to 10 μM (peptides S6_1). Respective results of the binding ability comparing experiment are shown in FIG. 7 (Synake) and FIG. 8 (peptides S6_1).