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Mouse il 10

Manufactured by Thermo Fisher Scientific
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Mouse IL-10 is a recombinant protein that serves as a quantitative standard for the detection and measurement of mouse interleukin-10 (IL-10) in biological samples. It can be used in various immunoassay techniques, such as ELISA, to determine the concentration of IL-10 in mouse-derived samples.

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Lab products found in correlation

Mouse il 6, by Thermo Fisher Scientific (3 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Mouse il 1β, by Thermo Fisher Scientific (2 mentions) Mouse il 4, by Thermo Fisher Scientific (2 mentions) Il 17a, by Thermo Fisher Scientific (1 mentions) Mouse il 6, by Dakewe (1 mentions) Elisa ready set go, by Thermo Fisher Scientific (1 mentions) Mouse il 6 elisa ready set go, by Thermo Fisher Scientific (1 mentions) Tlrl peklps, by InvivoGen (1 mentions) Mouse il 10 elisa set, by BD (1 mentions) Tlrl fsl, by InvivoGen (1 mentions) Mouse il 12 p70 elisa set, by BD (1 mentions) Tlrl pic, by InvivoGen (1 mentions) Mouse il 12 p40 elisa set, by BD (1 mentions) Tlrl pms, by InvivoGen (1 mentions) Mouse il 6 elisa set, by BD (1 mentions) Elx808 plate reader, by Agilent Technologies (1 mentions) Lps from escherichia coli serotype 0127 b8, by Merck Group (1 mentions) Ready set go elisa kit, by Thermo Fisher Scientific (1 mentions) Quantikine elisa mouse m csf, by R&D Systems (1 mentions)

Spelling variants of this product

IL-10 (509 citations) IL-10 Mouse Uncoated ELISA Kit (11 citations) Mouse IL-10 ELISA Ready-SET-Go! (11 citations) Mouse IL-10 ELISA (6 citations) Anti-mouse IL-10-FITC (2 citations) Mouse IL-10 platinum ELISA kit (2 citations) Mouse IL-10 ELISA Ready-SET&Go!® assay (2 citations) PE-conjugated anti-mouse IL-10 (JES5–16E3, (2 citations) Anti-mouse IL-10 (2 citations) Rat anti-mouse IL-10 (clone JES5–16E3; (2 citations)

13 protocols using mouse il 10

1

Cytokine Quantification in Mouse Samples

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The levels of mouse IL-6 (Dakewe, Shanghai, China), IL-17A (eBioscience), and mouse IL-10 (eBioscience) in the supernatant were detected with ELISA kits following the manufacturer's instructions. Every sample was measured in duplicate. Concentrations of the cytokines were calculated according to the standard curve.
Yang J., Wu J., Zhang R., Yao M., Liu Y., Miao L, & Sun W. (2016). Porphyromonas gingivalis oral infection promote T helper 17/Treg imbalance in the development of atherosclerosis. Journal of Dental Sciences, 12(1), 60-69.
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2

TLR Agonist-Induced Cytokine Profiling in Dendritic Cells

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CD4− MutuDC2s and MutuDC1s were seeded at a density of 2.5 × 105 cells/cm2 in 96-well or 48-well plates and incubated for 24 h with 450 μL/cm2 of the following TLR agonists diluted in complete medium: Pam3CSK4 (150 ng/mL, tlrl-pms, InvivoGen), poly(I:C) (8.5 μg/mL, tlrl-pic, InvivoGen), LPS from E. coli (100 ng/mL, tlrl-peklps, InvivoGen), ultrapure flagellin from B. subtilis (100 ng/mL, tlrl-pbsfla, InvivoGen), FSL-1 (100 ng/mL, tlrl-fsl, InvivoGen), Gardiquimod™ (1 μg/mL, tlrl-gdqs, InvivoGen), CpG ODN 1826 (1 μM, TriLink BIOTECHNOLOGIES). In all the experiments each condition was plated in technical triplicate. The supernatants were analyzed by ELISA for the presence of IL-6, IL-10, IL-12/IL-23 p40, IL-12p70, and MCP-1(CCL2) using the following kits according to manufacturer's instructions: Mouse IL-6 ELISA Set (555240, BD Biosciences) or Mouse IL-6 ELISA Ready-SET-Go! (88-7064, eBioscience), Mouse IL-10 ELISA Set (555252, BD Biosciences) or Mouse IL-10 (Interleukin-10) ELISA Ready-SET-Go! (88-7104, eBioscience), Mouse IL-12 (p40) ELISA Set (555165, BD Biosciences), Mouse IL-12 (p70) ELISA Set (555256, BD Biosciences), Mouse CCL2 (MCP-1) ELISA Ready-SET-Go! (88-7391, eBioscience).
Pigni M., Ashok D., Stevanin M, & Acha-Orbea H. (2018). Establishment and Characterization of a Functionally Competent Type 2 Conventional Dendritic Cell Line. Frontiers in Immunology, 9, 1912.
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3

Quantifying cytokine production in DCs

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DCs differentiated from bone marrow (bmDC) or iPSCs (ipDC) were cultured at 2 × 105 cells per well of 96 well tissue culture plates and stimulated with a serial dilution of lipopolysaccharide (LPS) from Escherichia coli Serotype 0127:B8 (Sigma‐Aldrich, Dorset, UK). After 22 hours, culture supernatants were harvested and concentrations of IL‐10 and IL‐12 determined by sandwich ELISA, according to the manufacturer's instructions (eBioscience Mouse IL‐10 [Cat# 88‐7104‐22], IL‐12 p70 [Cat# 88‐7121‐88] ELISA Ready‐SET‐Go! kits). Optical density (OD)450nm and OD570nm was determined using a BioTek ELX808 plate reader.
Horton C., Davies T.J., Lahiri P., Sachamitr P, & Fairchild P.J. (2019). Induced pluripotent stem cells reprogrammed from primary dendritic cells provide an abundant source of immunostimulatory dendritic cells for use in immunotherapy. Stem Cells (Dayton, Ohio), 38(1), 67-79.
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4

Glycoconjugates Modulate Cytokine Secretion

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The levels of interleukins and growth factors in cell culture supernates mediated by the exposure with glycoconjugates GM-1 and GM-2, respectively, were assayed with Quantikine ELISA® Mouse M-CSF [Cat#MMC00, R&D, USA, minimum detectable dose (MDD) <5 pg/mL], Platinum ELISAs®: Mouse IL-12 (p70) (Cat#BMS616, MDD 4 pg/mL), Mouse GM-CSF (Cat#BMS612, MDD 2 pg/mL), Mouse IL-17(Cat#BMS6001, MDD 1.6 pg/mL), Mouse IL-2(Cat#BMS601, MDD 5.3 pg/mL), and Mouse IL-6 (Cat#BMS603/2, MDD 6.5 pg/mL); Instant ELISAs®: Mouse IL-1β (Cat#BMS600/2INST, MDD 3 pg/mL), Mouse tumor necrosis factor (TNF)-α (Cat#BMS607/2INST, MDD 4 pg/mL), Mouse IL-10 (Cat#BMS614INST, MDD 5.28 pg/mL), all from Affymetrix e-Bioscience, USA, according to the instructions of the manufacturer.
Paulovičová E., Paulovičová L., Hrubiško M., Krylov V.B., Argunov D.A, & Nifantiev N.E. (2017). Immunobiological Activity of Synthetically Prepared Immunodominant Galactomannosides Structurally Mimicking Aspergillus Galactomannan. Frontiers in Immunology, 8, 1273.
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5

In Vitro Cytokine Modulation

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Mouse rGM-CSF and rIL-4 were purchased from Peprotech (Rocky hill, NJ, USA). CFSE, 2-ME, l-glutamine, and sodium pyruvate were purchased from Invitrogen (Carlsbad, CA, USA). CoPP and tin protoporphyrin (SnPP) were purchased from Phophyrin Products (Logan, UT, USA). LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse IL-10 and IFN-γ ELISA kits were purchased from eBioscience (San Diego, CA, USA).
Zhao Y., Jia Y., Wang L., Chen S., Huang X., Xu B., Zhao G., Xiang Y., Yang J, & Chen G. (2018). Upregulation of Heme Oxygenase-1 Endues Immature Dendritic Cells With More Potent and Durable Immunoregulatory Properties and Promotes Engraftment in a Stringent Mouse Cardiac Allotransplant Model. Frontiers in Immunology, 9, 1515.
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6

Measuring Inflammatory Cytokines and Enzymes

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Tumor necrosis factor (TNF)-α (human and mice), IL-6 (human and mice), IL-10 (human and mice), CXCL1, CXCL2, CXCL5 (all except mouse IL-10: R&D Systems, Minneapolis, MN, USA; mouse IL-10: eBioscience, Thermo Fisher Scientific), elastase, and myeloperoxidase (MPO) (Hycult Biotechnology BV, Uden, the Netherlands) were measured via ELISA according to manufacturers’ recommendations.
Saris A., Qin W., van Linge C.C., Reijnders T.D., Florquin S., Burnet M., Strass S., de Vos A.F, & van der Poll T. (2022). The Azithromycin Pro-Drug CSY5669 Boosts Bacterial Killing While Attenuating Lung Inflammation Associated with Pneumonia Caused by Methicillin-Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 66(9), e02298-21.
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7

Cytokine Detection in Cell-Free Supernatants

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Cell-free supernatants were harvested and assayed for mouse IL-10 (eBioscience) and IL-1β (eBioscience) according to the manufacturer’s instructions.
Li J., Lu L., Binder K., Xiong J., Ye L., Cheng Y.H., Majri-Morrison S., Lu W., Lee J.W., Zhang Z., Wu Y.Z., Zheng L, & Lenardo M.J. (2023). Mechanisms of antigen-induced reversal of CNS inflammation in experimental demyelinating disease. Science Advances, 9(9), eabo2810.
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8

Quantifying Secreted IL-10 by ELISA

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Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader. The concentration of supernatant IL-10 was determined using the manufacturer’s standard curve over the range of 32–4000 pg/mL.
Benakis C., Simats A., Tritschler S., Heindl S., Besson-Girard S., Llovera G., Pinkham K., Kolz A., Ricci A., Theis F.J., Bittner S., Gökce Ö., Peters A, & Liesz A. (2022). T cells modulate the microglial response to brain ischemia. eLife, 11, e82031.
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9

Quantifying Cytokine Levels in BMDM

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The levels of cytokines present on cell-free supernatant of stimulated BMDM were evaluated with the Invitrogen kit according to manufacturer's instructions: mouse TNF-α (cat # 88-7324-88); mouse IL-10 (cat # 88-7105-88); mouse IL-1 β (cat # 88-7013-88), and mouse IL-6 (cat # 88-7064-88).
Alves A.L., Costa-Gouveia J., Vieira de Castro J., Sotelo C.G., Vázquez J.A., Pérez-Martín R.I., Torrado E., Neves N., Reis R.L., Castro A.G, & Silva T.H. (2022). Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications. Acta biomaterialia, 141.
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10

Cytokine Quantification in Serum

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Concentrations of interleukin-1β (IL-1β), IL-6, IL-10, and tumor necrosis factor-α (TNFα) in serum samples were measured using a mouse IL-1 beta, mouse IL-6, mouse IL-10, and mouse TNF alpha uncoated ELISA kits, respectively (Thermo Fisher Scientific), according to the manufacturers’ instructions.
Pal R., Bradford B.M, & Mabbott N.A. (2022). Innate Immune Tolerance in Microglia Does Not Impact on Central Nervous System Prion Disease. Frontiers in Cellular Neuroscience, 16, 918883.
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