Electronic absorption data were collected with a Hewlett-Packard 8452A diode-array spectrophotometer. Unless otherwise stated, samples were removed from the NMR tube after NMR data collection, thoroughly mixed and transferred to a disposable cuvette (14–878, Fisher Scientific, Pittsburgh, PA) for electronic absorption data collection. Some absorption spectra were also recorded directly in the NMR tube as described22 (link). Briefly, the NMR tube containing the sample of interest was inserted in a disposable cuvette (14–955, Fisher Scientific, Pittsburgh, PA) and secured to the bottom of the cuvette via a glued NMR cap. The interstitial space between the cuvette and the NMR tube was then filled with water22 (link).
Disposable cuvette
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Disposable cuvettes are single-use containers made of transparent material, commonly plastic or glass. They are designed for spectrophotometric analysis, enabling the measurement of light absorption or transmission through a sample. Disposable cuvettes provide a convenient and controlled environment for various analytical applications in laboratories.
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Lab products found in correlation
6 protocols using disposable cuvette
1
Spectroscopic Characterization of Samples
2
Exebacase Treatment of Bacterial Growth
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Overnight cultures of MW2 and CFS-1155 were diluted 1:100 in TSB and grown at 37°C with aeration. At OD600 1.0 (~5 × 108 CFU/mL), 0.8 mL was transferred into Fisherbrand Disposable Cuvettes (1.5 mL semi-micro cell, polystyrene, catalog no. 14–955-127). For treated cultures, exebacase was added to final concentration of 64 µg/mL. For untreated cultures, PBS alone was added. Cultures were immediately visualized using an MP4 video format on a Nikon Coolpix B500 camera set at 1080 resolution, 30 FPS, 29 min (maximal time allowed). The resulting video was cropped, and playback speed was adjusted to cover the first 15 min in a 14-s video with resolution set to 720 p.
3
Bradford Assay for Protein Quantification
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The protein concentrations of the samples were determined using the Bradford Assay—Sigma [22 ]. Bovine serum albumin (BSA- Fisher Scientific) standards of known protein concentration varying from 0 to 2 mg/ml were added to Bradford reagent in disposable cuvettes (Fisher Scientific) and the absorbance was measured spectrophotometrically at 595nm in a Thermo Scientific Spectrometer Helios Episilon (Fisher Scientific). The absorbance of the known protein standards were plotted using a linear regression. The mitochondrial fractions were diluted 1 μl in 100 μl Tris Buffer and added to Bradford reagent before the absorbance was measured. The absorbance was plotted against the BSA standard curve to determine the estimated protein concentration (mg/ml).
4
Exebacase Treatment of Bacterial Growth
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Overnight cultures of MW2 and CFS-1155 were diluted 1:100 in TSB and grown at 37°C with aeration. At OD600 1.0 (~5 × 108 CFU/mL), 0.8 mL was transferred into Fisherbrand Disposable Cuvettes (1.5 mL semi-micro cell, polystyrene, catalog no. 14–955-127). For treated cultures, exebacase was added to final concentration of 64 µg/mL. For untreated cultures, PBS alone was added. Cultures were immediately visualized using an MP4 video format on a Nikon Coolpix B500 camera set at 1080 resolution, 30 FPS, 29 min (maximal time allowed). The resulting video was cropped, and playback speed was adjusted to cover the first 15 min in a 14-s video with resolution set to 720 p.
5
Protein Quantification using Bradford Assay
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The protein concentrations of the samples were determined using the Bradford Assay - Sigma [64 ]. Bovine serum albumin (BSA- Fisher Scientific) standards of known protein concentration varying from 0 to 2mg/ml were added to Bradford reagent in disposable cuvettes (Fisher Scientific) and the absorbance was measured spectrophotometrically at 595 nm in a Thermo Scientific Spectrometer Helios Episilon (Fisher Scientific). The absorbance of the known protein standards was plotted using linear regression. The mitochondrial fractions were diluted 1 μl in 100 μl Tris Buffer and added to Bradford reagent before the absorbance was measured. The absorbance was plotted against the BSA standard curve to determine the estimated protein concentration (mg/ml).
6
Characterizing AgNPs Size and Polydispersity
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The size of AgNPs and their polydispersity index (PDI) were determined using dynamic light scattering (DLS) with a Zetasizer Nano-ZS (Malvern Instruments, Manchester, UK; model: ZEN3600, serial number: MAL1000973) and disposable cuvettes (Fisherbrand, Loughborough, UK; FB55147). Prior to measurement, each sample was filtered by means of a 0.22 µm PTFE filter. After filtration, these samples were measured without dilution and with 10-fold dilution using ultrapure HPLC-grade water (Sigma Aldrich, Gillingham, UK; CAS No: 7732-18-5; PCode: 102604938; Source: BCCK3219). Each sample was measured three times at 25 °C, and the mean ± standard deviation values were calculated.
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