2, 2-Diphenyl-1-picrylhydrazyl (DPPH), trichloroacetic acid (TCA), 2,4-dinitrophenyl hydrazine (DNPH), 2,4,6-Tripyridyl-s-triazine (TPTZ), ferric chloride (FeCl3), nitroblue tetrazolium (NBT), nicotinamide adenine dinucleotide (NADH), phenazine metho-sulfate (PMS), Dulbecco's modified Eagle medium (DMEM), (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), sulfanilamide, N-(1-Naphthyl) ethylenediamine (NED), 2’,7’-dichlorofluorescein diacetate (DCFDA), lipopolysaccharide (LPS), and all other chemicals used were purchased from Merck, HiMedia, and Sigma-Aldrich. The high-performance-thin-layer chromatography (HP-TLC) plates Si 60F254 (20 * 10) were purchased from E. Merck (Darmstadt, Germany).
Si 60 f254
Manufactured by Merck Group
Sourced in Germany, United States
The Si 60 F254 is a type of silica gel thin-layer chromatography (TLC) plate. It is designed for the separation and analysis of a wide range of chemical compounds. The plate is coated with a fluorescent indicator that allows for the visualization of separated components under ultraviolet (UV) light.
Automatically generated - may contain errors
Lab products found in correlation
Sephadex lh 20, by Merck Group (3 mentions)
Fisher johns apparatus, by Thermo Fisher Scientific (3 mentions)
Si 60, by Merck Group (2 mentions)
Avance 400, by Bruker (2 mentions)
Chloroform, by Cambridge Isotopes (2 mentions)
Rutin, by Merck Group (2 mentions)
Polyamide 6, by Merck Group (2 mentions)
Deuterated methanol, by Cambridge Isotopes (2 mentions)
Folin ciocalteu, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Avance 3 400 mhz, by Bruker (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Sodium nitrite, by Merck Group (2 mentions)
Avance 300 mhz, by Bruker (2 mentions)
Silica gel 60, by Merck Group (2 mentions)
Avance 500 mhz spectrometer, by Bruker (2 mentions)
Sulfanilamide, by Merck Group (1 mentions)
20 protocols using si 60 f254
1
Antioxidant and Cytotoxicity Assays
2
General Synthetic Protocol Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All available
chemicals and solvents
were purchased from commercial sources and were used without any further
purification. Thin-layer chromatography (TLC) was performed using
0.25 mm silica gel precoated plates Si 60-F254 (Merck, Darmstadt,
Germany) visualized by UV-254 light and cerium ammonium molybdate
(CAM) staining. Purification by flash column chromatography (FCC)
was conducted by using silica gel Si 60, 230–400 mesh, 0.040–0.063
mm (Merck). Melting points were determined on a Stuart Scientific
SMP3 and are corrected. 1H and 13C NMR spectra
were recorded on a Bruker Avance 400 (400 and 101 MHz, respectively);
chemical shifts are indicated in parts per million downfield from
SiMe4, using the residual proton (CHCl3 = 7.27
ppm) and carbon (CDCl3 = 77.0 ppm) solvent resonances as
an internal reference. Coupling constant values J are given in Hz. High-resolution mass spectra (HRMS) were recorded
using a mass spectrometer MicroTOF from Bruker with an electron spray
ion source (ESI) and a TOF detector or using a mass spectrometer from
Thermo Fisher Scientific with an electron spray ion source (ESI) and
a LTQ Orbitrap as a detector. FTIR spectra were recorded on a Tensor
27 (ATR Diamond) Bruker infrared spectrophotometer and are reported
in frequency of absorption (cm–1).
chemicals and solvents
were purchased from commercial sources and were used without any further
purification. Thin-layer chromatography (TLC) was performed using
0.25 mm silica gel precoated plates Si 60-F254 (Merck, Darmstadt,
Germany) visualized by UV-254 light and cerium ammonium molybdate
(CAM) staining. Purification by flash column chromatography (FCC)
was conducted by using silica gel Si 60, 230–400 mesh, 0.040–0.063
mm (Merck). Melting points were determined on a Stuart Scientific
SMP3 and are corrected. 1H and 13C NMR spectra
were recorded on a Bruker Avance 400 (400 and 101 MHz, respectively);
chemical shifts are indicated in parts per million downfield from
SiMe4, using the residual proton (CHCl3 = 7.27
ppm) and carbon (CDCl3 = 77.0 ppm) solvent resonances as
an internal reference. Coupling constant values J are given in Hz. High-resolution mass spectra (HRMS) were recorded
using a mass spectrometer MicroTOF from Bruker with an electron spray
ion source (ESI) and a TOF detector or using a mass spectrometer from
Thermo Fisher Scientific with an electron spray ion source (ESI) and
a LTQ Orbitrap as a detector. FTIR spectra were recorded on a Tensor
27 (ATR Diamond) Bruker infrared spectrophotometer and are reported
in frequency of absorption (cm–1).
3
Chromatographic Analysis of Natural Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silica gel 60 (particle size 0.063–0.2 mm, 70–230 mesh) (Fluka, St. Louis, MO, USA), Polyamide-6 and Sephadex LH-20 (Sigma-Aldrich, Taufkirchen, Germany) were utilized for column chromatography. TLC plates (Si 60 F254, Merck, Darmstadt, Germany) and analytical-grade solvents were used in the study. Visualization of the spots was carried out using p-anisaldehyde spray reagent [37 ].
The UV investigation was carried out in methanol utilizing a Shimadzu UV1, 601PC UV–visible scanning spectrophotometer (Shimadzu Corp., Tokyo, Japan). Optical rotation was measured using a 341 Perkin Elmer polarimeter (Darmstadt, Germany). HRESIMS was executed on Agilent LC/Q-TOF, 6530 (Santa Clara, CA, USA). All 1D and 2D NMR spectra were recorded on a Bruker Avance III 400 MHz (Bruker AG, Fällanden, Switzerland) and analysed using Topspin 3.1 software (Bruker AG, Fällanden, Switzerland). Deuterated methanol and chloroform (Cambridge Isotopes, Andover, MA, USA) were used.
Aluminium trichloride (AlCl3.6H2O), anhydrous sodium carbonate (Na2CO3), ascorbic acid, DPPH, ABTS (Sigma, PN: A3219, St. Louis, MO, USA), Folin–Ciocalteu, gallic acid, rutin, sodium hydroxide (NaOH), and sodium nitrite (NaNO2) were purchased from Merck (Rahway, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA).
The UV investigation was carried out in methanol utilizing a Shimadzu UV1, 601PC UV–visible scanning spectrophotometer (Shimadzu Corp., Tokyo, Japan). Optical rotation was measured using a 341 Perkin Elmer polarimeter (Darmstadt, Germany). HRESIMS was executed on Agilent LC/Q-TOF, 6530 (Santa Clara, CA, USA). All 1D and 2D NMR spectra were recorded on a Bruker Avance III 400 MHz (Bruker AG, Fällanden, Switzerland) and analysed using Topspin 3.1 software (Bruker AG, Fällanden, Switzerland). Deuterated methanol and chloroform (Cambridge Isotopes, Andover, MA, USA) were used.
Aluminium trichloride (AlCl3.6H2O), anhydrous sodium carbonate (Na2CO3), ascorbic acid, DPPH, ABTS (Sigma, PN: A3219, St. Louis, MO, USA), Folin–Ciocalteu, gallic acid, rutin, sodium hydroxide (NaOH), and sodium nitrite (NaNO2) were purchased from Merck (Rahway, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA).
4
Detailed Synthetic Protocols for Diverse Compounds
5
Synthesis and Characterization of Phthalonitrile Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercially available reagents were used as received. 4-(diphenylamino)phthalonitrile 1 and pentyl 3,4-dicyanobenzoate 2 were prepared as described in our preceding works18 ,35 (link). Solvents were purified by standard methods and dried if necessary. Reactions were monitored by thin layer chromatography (TLC) that was conducted on plates precoated with silica gel Si 60-F254 (Merck, Germany). Column chromatography was conducted using silica gel Si 60, 0.063–0.200 mm (Merck, Darmstadt, Germany). UV-Vis measurements in solution were performed on a Nicolet Evolution 500 spectrophotometer (Thermo Electron Corporation). FTIR were performed on a Cary 530 instrument (Agilent Technologies). ESI mass spectra were obtained with a ICR-FTMS APEX II (Bruker Daltonics) mass spectrometer. MALDI mass spectra were obtained with a TOF-TOF mass spectrometer Microflex LT (Bruker). Elemental analyses were carried out by the Departmental Service of Microanalysis (University of Milano).
6
Physicochemical Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting points were determined using Fisher-Johns apparatus (Fisher Scientific, Schwerte, Germany) and were not corrected. The 1H NMR and 13 C NMR spectra were recorded by a Bruker Avance 300 MHz instrument using DMSO-d6 as solvent and TMS as an internal standard. Chemical shifts were expressed as δ (ppm). MS using atmospheric pressure chemical ionisation (APCI) was recorded on a Bruker MicroTOF II mass spectrometer. APCI settings were as follows: vaporiser temperature, 350 °C; drying gas temperature, 180 °C; drying gas flow, 4 l/min; and nebuliser pressure, 2 bar. The purity of the compounds was checked by TLC on plates with silica gel Si 60 F254, produced by Merck Co. (Darmstadt, Germany). Elemental analyses were performed by AMZ 851 CHX analyser and the results were within ±0.4% of the theoretical value.
7
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organic solvents for extraction and chromatographic separation were obtained from Active Fine Chemicals, Bangladesh. Melting points were determined on a digital melting point Apparatus of Cole-Parmer Ltd., UK (model SMP10). Thin layer chromatography (TLC) was run on Merck pre-coated TLC plates with Si60F254. Plates were visualized by spraying with Lieberman–Burchard reagent followed by heating. Vacuum Liquid chromatography was done using Silica gel 60 (0.040–0.005 mm), Merck, Germany. Open column chromatography was performed using Silica gel 60 (0.063–0.020 mm), Merck, Germany. Spectral data were obtained as follows: Infrared (IR) spectrum with a Shimadzu IR Prestige-2 FT-IR spectrophotometer, ultraviolet spectrum with a Shimadzu UV spectrophotometer (UV-1800), nuclear magnetic resonance (NMR) spectra with an ultra-shield Bruker Avance 400 MHZ in CD3OD. The NMR spectra were recorded running gradients and using residual solvent peak (at 3.33 in 1H-NMR and middle peak of septate at 49.0 in 13C-NMR) as internal reference.
8
Chromatographic Analysis of Natural Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silica gel 60 (particle size 0.063–0.2 mm, 70–230 mesh) (Fluka, St. Louis, MO, USA), Polyamide-6 and Sephadex LH-20 (Sigma-Aldrich, Taufkirchen, Germany) were utilized for column chromatography. TLC plates (Si 60 F254, Merck, Darmstadt, Germany) and analytical-grade solvents were used in the study. Visualization of the spots was carried out using p-anisaldehyde spray reagent [37 ].
The UV investigation was carried out in methanol utilizing a Shimadzu UV1, 601PC UV–visible scanning spectrophotometer (Shimadzu Corp., Tokyo, Japan). Optical rotation was measured using a 341 Perkin Elmer polarimeter (Darmstadt, Germany). HRESIMS was executed on Agilent LC/Q-TOF, 6530 (Santa Clara, CA, USA). All 1D and 2D NMR spectra were recorded on a Bruker Avance III 400 MHz (Bruker AG, Fällanden, Switzerland) and analysed using Topspin 3.1 software (Bruker AG, Fällanden, Switzerland). Deuterated methanol and chloroform (Cambridge Isotopes, Andover, MA, USA) were used.
Aluminium trichloride (AlCl3.6H2O), anhydrous sodium carbonate (Na2CO3), ascorbic acid, DPPH, ABTS (Sigma, PN: A3219, St. Louis, MO, USA), Folin–Ciocalteu, gallic acid, rutin, sodium hydroxide (NaOH), and sodium nitrite (NaNO2) were purchased from Merck (Rahway, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA).
The UV investigation was carried out in methanol utilizing a Shimadzu UV1, 601PC UV–visible scanning spectrophotometer (Shimadzu Corp., Tokyo, Japan). Optical rotation was measured using a 341 Perkin Elmer polarimeter (Darmstadt, Germany). HRESIMS was executed on Agilent LC/Q-TOF, 6530 (Santa Clara, CA, USA). All 1D and 2D NMR spectra were recorded on a Bruker Avance III 400 MHz (Bruker AG, Fällanden, Switzerland) and analysed using Topspin 3.1 software (Bruker AG, Fällanden, Switzerland). Deuterated methanol and chloroform (Cambridge Isotopes, Andover, MA, USA) were used.
Aluminium trichloride (AlCl3.6H2O), anhydrous sodium carbonate (Na2CO3), ascorbic acid, DPPH, ABTS (Sigma, PN: A3219, St. Louis, MO, USA), Folin–Ciocalteu, gallic acid, rutin, sodium hydroxide (NaOH), and sodium nitrite (NaNO2) were purchased from Merck (Rahway, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA).
9
Purification and Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All commercial reactants and solvents were purchased from either Alfa Aesar (Kandel, Germany) or Sigma-Aldrich (St. Louis, MO, USA) with the highest purity and used without further purification. The melting points were determined by using Fisher-Johns apparatus (Fisher Scientific, Schwerte, Germany) and are uncorrected. The purity of the compounds was checked by TLC on plates with silica gel Si 60F254, produced by Merck Co. (Darmstadt, Germany). The 1H-NMR and 13C-NMR spectra were recorded by a Bruker Avance 300 MHz instrument using DMSO-d6 as solvent and TMS as an internal standard. Chemical shifts were expressed as δ (ppm). Elemental analyses were performed by AMZ 851 CHX analyzer and the results were within ±0.4% of the theoretical value.
10
α-Glucosidase Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rice α-glucosidase (Type 4) was purchased from Sigma Aldrich, St. Louis City, MO, USA. Saccharomyces cerevisiae (yeast), B. stearothermophilus α-glucosidases and acarbose were obtained from Sigma Chemical Co., St. Louis City, MO, USA. Rat α-glucosidase was provided by Sigma Aldrich, Singapore. p-nitrophenyl glucopyranoside (pNPG) was purchased from Sigma Aldrich, 3050 Spruce Street, St. Louis, MO, USA. 1H-NMR (500 MHz) and 13C-NMR (125 MHz) were measured on a Bruker Avance 500 MHz spectrometer. Column chromatography was carried out on silica gel (Si 60 F254, 40–63 mesh, Merck, St. Louis, MO, USA). All solvents were redistilled before use. Pre-coated TLC plates (Si 60 F254) were used for analytical purposes. Compounds were visualized under UV radiation (254, 365 nm) and by spraying plates with 10% H2SO4, followed by heating with a heat gun.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!