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4 protocols using ab214091

1

Biomarker Quantification in Plasma

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Commercially available ELISA kits were used to measure the concentrations or activity of IFNβ (DIFNB0 or 42400–1, R&D Systems), F3 (DCF300, R&D Systems; ab214091, Abcam), fibrin (MBS265263 or MBS706338, MyBioSource), D-dimer (ab196269, Abcam; MBS723281, MyBioSource), TNF (DTA00D, R&D Systems), IL1A (DLA50, R&D Systems), IL1B (DLB50, R&D Systems), IL6 (D6050, R&D Systems), and HMGB1 (ST51011, IBL International) in the indicated samples. Measurement of GPT/ALT and BUN in the plasma was performed using an IDEXX Catalyst Dx Chemistry Analyzer. PT, APTT, and fibrinogen were measured in an automated coagulometer (Sysmex CA-7000). Platelet count was measured using an IDEXX ProCyte Dx Hematology Analyzer.
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2

Plasma Fibrinogen and TAT Complexes Quantification

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Commercial enzyme-linked immunosorbent assay [ELISA] kits from Abcam (Paris, France) were used to measure plasma (PFP) concentrations of fibrinogen (ref: ab108844) or TAT complexes (ref: ab137994). TF in aorta and kidney was measure by the commercial ELISA kit according to manufacturer's instructions (Abcam, ref: ab214091).
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3

Measuring Coagulation Factors in Thrombosis

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The activities of MMP-9, NF-κB, tissue factor (TF), TAFI and PAI-1 in vein endothelial cells and deep venous thrombus were analyzed by commercialized kits [MMP (ab100732; Abcam), NF-κB (JK-(a)-6261; Jiangkang Bioscience, Shanghai, China), TF (ab214091; Abcam), TAFI (MA143023; Beinuo Bioscience, Shanghai, China), PAI-I (ab197752; Abcam)] and performed according to the manufacturer's instructions.
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4

BALF Thrombin-Antithrombin and Tissue Factor Quantification

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BALF thrombin–antithrombin and tissue factor were measured using ELISA kits (abcam, UK—ab137994 and ab214091 respectively) according to manufacturer protocol.
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