Skyscan 1276 system

Manufactured by Bruker

Sourced in Belgium, Germany, United States

The SkyScan 1276 system is a high-resolution, non-destructive, in-vivo micro-CT imaging system designed for small animal imaging. It provides high-quality 3D images and allows for the visualization and quantification of anatomical structures and biological processes.

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Lab products found in correlation

Ctan software, by Bruker (5 mentions) Nrecon software, by Bruker (4 mentions) Dataviewer, by Bruker (2 mentions) Ctvol software, by Bruker (2 mentions) Recon software, by Bruker (1 mentions) Amira 5, by Visage Imaging (1 mentions) Paraformaldehyde (pfa), by Wuhan Servicebio Technology (1 mentions) Ctvox, by Bruker (1 mentions) Ivis lumina s5, by PerkinElmer (1 mentions) Ctvol, by Bruker (1 mentions) Living image software, by PerkinElmer (1 mentions) Skyscan software, by Bruker (1 mentions) Micro ct software, by Bruker (1 mentions) Amira software, by Thermo Fisher Scientific (1 mentions)

15 protocols using skyscan 1276 system

Micro-CT Analysis of Bone Regeneration

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Rats undergoing limb muscle punch surgery were anesthetized by 1% pentobarbital (3 mL kg⁻¹) at 5 weeks after operations, which were tested by X-ray. They were then sacrificed to obtain limb samples containing implanted scaffolds. After being fixed with 4% paraformaldehyde, the samples were tested by a micro-CT imaging system (SkyScan 1276 system, Bruker, Germany) under a source voltage of 45 kV, a current of 200 μA, and an aluminum filter of 0.25 mm with an image pixel size of 6.5 μm. N Recon software (Bruker, USA) was used to reconstruct the scanned images. DataViewer (Bruker, USA) and CTAn software (Bruker, USA) were used to analyze the newly formed bone volume (BV).
Rats undergoing surgery for critical calvarial defects were sacrificed at 8 weeks to obtain calvarial samples containing defects. The samples were fixed with 4% paraformaldehyde and then examined by a micro-CT imaging system (SkyScan 1276 system, Bruker, Germany) under a source voltage of 45 kV, a current of 200 μA, and an aluminum filter of 0.25 mm with an image pixel size of 6.5 μm. The raw data were reconstructed by Recon software (Bruker, USA). The parameters reflecting bone regeneration were analyzed by DataViewer (Bruker, USA) and CTAn software (Bruker, USA), which included the newly formed BV, BV/tissue volume (BV/TV), trabecular number (Tb. N), and trabecular spacing (Tb.Sp).

Hao Z., Feng Q., Wang Y., Wang Y., Li H., Hu Y., Chen T., Wang J., Chen R., Lv X., Yang Z., Chen J., Guo X, & Li J. (2023). A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration. Bioactive Materials, 34, 181-203.

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Micro-CT Analysis of Calvarial Tissues

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The fixed calvarial tissues were analyzed using the micro-CT Skyscan 1276 system (Bruker, Germany). The scanning protocol was set at an isotropic voxel size of 6.53 μm, X-ray energy settings of 85 kV and 200 uA, and an exposure time of 400 ms, with a rotation in equiangular steps of 0.9° following an angle of 180°. The wear particles were removed before scanning to avoid metal artifacts. Reconstruction was accomplished by Nrecon (version 1.7.3.0). A volume of interest (VOI) of 3 mm × 3 mm × 1 mm was selected for further quantitative analysis. The bone volume to tissue volume ratio (BV/TV), bone mineral density (BMD), and total porosity of each sample were obtained using CT Analyser software (Skyscan). The experiment was performed in triplicate.

Zhao Z., Wang C., Xu Y., Wang X., Jia B., Yu T., Wang Y, & Zhang Y. (2021). Effects of the Local Bone Renin-Angiotensin System on Titanium-Particle-Induced Periprosthetic Osteolysis. Frontiers in Pharmacology, 12, 684375.

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Micro-CT Evaluation of Bone-Implant Interface

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The femurs containing implants were scanned via the μ-CT Skyscan 1276 system (Bruker, Germany). Scan settings are as follows: voxel size 9.0 μm, energy setting 85 kV, 200 μA, 1 mm Al filter and integration time 384 ms. Reconstruction was carried out by NRecon software (version 1.7.4.2). 3D images were obtained from contoured 2D images by methods based on distance transformation of the grayscale original images (CTvox; version 3.3.0). CT Analyser (version 1.18.8.0) was used to analyze the region of interest (ROI), including the 200 μm annular region extending around the implant. The bone-implant contact (BIC), total volume (TV), bone volume (BV), volume ratio (BV/TV), bone surface (BS), bone mineral density (BMD), trabecular bone number (Tb.N), trabecular separation (Tb.Sp), and trabecular thickness (Tb.Th) were calculated by software.

Chen Z., Chen Y., Wang Y., Deng J., Wang X., Wang Q., Liu Y., Ding J, & Yu L. (2023). Polyetheretherketone implants with hierarchical porous structure for boosted osseointegration. Biomaterials Research, 27, 61.

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Micro-CT Imaging of Skeletal Tissues

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The retrieved subcutaneous samples and calvarial specimens were fixed in 4 % paraformaldehyde, and imaged with the Bruker Micro-CT Skyscan 1276 system (Kontich, Belgium) as described [[80] , [81] (link), [82] ]. The scanning parameters were as follows: voxel size 6.533712 μm, medium resolution, 85 kV, 200 μA, 1 mm Al filter, and integration time 384 ms. Density measurements were calibrated to the manufacturer's calcium hydroxyapatite (CaHA) phantom. Data analysis was performed using the manufacturer's evaluation software. Reconstruction was accomplished by NRecon (version 1.7.4.2). The 3-D images were obtained from contoured 2D images based on distance transformation of the grayscale original images (CTvox; version 3.3.0). 3D analysis was performed using software CT Analyzer (version 1.18.8.0) or Amira 5.3 (Visage Imaging, Inc.).

Gou Y., Huang Y., Luo W., Li Y., Zhao P., Zhong J., Dong X., Guo M., Li A., Hao A., Zhao G., Wang Y., Zhu Y., Zhang H., Shi Y., Wagstaff W., Luu H.H., Shi L.L., Reid R.R., He T.C, & Fan J. (2023). Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering. Bioactive Materials, 34, 51-63.

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Maxillary Bone Density Analysis

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Right maxillary samples were fixed with 4% paraformaldehyde (Servicebio, Hubei, China) for 2 days at 4 °C, transferred to 70% alcohol, scanned using a SkyScan1276 system (BRUKER, MA, USA) at 10-μm scaled image pixel size with an energy level of 55 kV. The scans were reconstructed to produce three-dimensional images by CTvox (BRUKER, MA, USA) and two-dimensional section images by DataViewer (BRUKER, MA, USA). The extraction socket of the first molar was isolated by manual contouring and analyzed with regard to bone volume/tissue volume, bone mineral density and trabecular parameter (trabecular thickness, trabecular number, trabecular separation) by CTan software (BRUKER, MA, USA) at the consistent beginning grayscale index of 4.7.

Meng Z., Liu J., Feng Z., Guo S., Wang M., Wang Z., Li Z., Li H, & Sui L. (2022). N-acetylcysteine regulates dental follicle stem cell osteogenesis and alveolar bone repair via ROS scavenging. Stem Cell Research & Therapy, 13, 466.

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High-Resolution X-ray Microtomography of Brain Tissue

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Brain samples were scanned on a Bruker Skyscan 1276 system with voltage 50 kV, current 100 μA, and a 0.25 mm Al filter. Image capture was set with a pixel size of 6 μm. Per scan, we acquired an average of 2,100 projection images at a 0.2° rotation step over 360° total to improve the signal-to-noise ratio. Reconstruction of the data was generated with NRecon software (Bruker) for .bmp files for downstream processing by Imaris (9.7.2, Bitplane). Additional information on visualization and quantitative analysis is provided in Supplemental Methods.

Romay M.C., Knutsen R.H., Ma F., Mompeón A., Hernandez G.E., Salvador J., Mirkov S., Batra A., Sullivan D.P., Procissi D., Buchanan S., Kronquist E., Ferrante E.A., Muller W.A., Walshon J., Steffens A., McCortney K., Horbinski C., Tournier‑Lasserve E., Sonabend A.M., Sorond F.A., Wang M.M., Boehm M., Kozel B.A, & Iruela-Arispe M.L. (2024). Age-related loss of Notch3 underlies brain vascular contractility deficiencies, glymphatic dysfunction, and neurodegeneration in mice. The Journal of Clinical Investigation, 134(2), e166134.

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Micro-CT Imaging of Silicone Tubing

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Micro-CT analyses were performed on the SkyScan 1276 System (Bruker). The mice were anesthetized using 3% isoflurane in oxygen, then were placed in an exchangeable animal cassette, and maintained the anesthesia throughout the whole scanning process. During the scans, the x-ray source was set to a voltage of 80 kV and a current of 200 μA. The images were obtained using a binning mode of 2 × 2. The resolution of the obtained images is around 20 μm per pixel. The obtained images were reconstructed into 2D transverse cross sections using NRecon software (version 1.7.4.2). Subsequently, 3D reconstruction was performed using Avizo software (version 8.1.1). A segmentation process was conducted to visualize the maintained hollow structure of the silicone tubing based on the different absorption contrasts between the gas phase and the silicone tubing, hydrogel, and mouse tissue.

Wang L.H., Ernst A.U., Flanders J.A., Liu W., Wang X., Datta A.K., Epel B., Kotecha M., Papas K.K, & Ma M. (2021). An inverse-breathing encapsulation system for cell delivery. Science Advances, 7(20), eabd5835.

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Microstructural Analysis of Bone Samples

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Samples are scanned using the SkyScan 1276 system (Bruker) microCT. The operation condition was 60 kV, 200 μA and a 0.25 mm filter of aluminum. 3D parametric analysis was performed within the region of interest (ROI) using CTAn software (Bruker). We calculated the bone volume in the total explant space as defined by the ROI as well as the inside scaffold volume only.

Cui Y., Ji W., Gao Y., Xiao Y., Liu H, & Chen Z. (2021). Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity. International Journal of Oral Science, 13, 44.

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Multimodal Imaging of Organ Pathology

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Freshly harvested organs were fluorescently imaged using the IVIS Lumina S5 platform (PerkinElmer). Images that were used for direct comparisons were acquired using the same field of view, exposure time, pixel binning and f-stop settings and analysed by the Living image software (PerkinElmer). For µCT analysis, 4% paraformaldehyde/PBS solution fixed bones were scanned with the Bruker Skyscan 1276 system at a resolution of 17.56 µm/pixel. 3D structural analyses were completed using the CTvol and CTAn software (Bruker).

Yip R.K., Rimes J.S., Capaldo B.D., Vaillant F., Mouchemore K.A., Pal B., Chen Y., Surgenor E., Murphy A.J., Anderson R.L., Smyth G.K., Lindeman G.J., Hawkins E.D, & Visvader J.E. (2021). Mammary tumour cells remodel the bone marrow vascular microenvironment to support metastasis. Nature Communications, 12, 6920.

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Micro-CT Analysis of Trabecular Bone

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For the analysis of trabecular bone microstructure, we employed the micro-CT SkyScan 1276 system (Bruker, Kontich, Belgium) to perform scans on the distal femoral bone. The reconstruction of the scanned images was carried out using the NRecon software (version 1.7.42, Bruker). Three-dimensional images of the distal femoral bone were generated using CTvol software (version 2.3.2.0, Bruker). The volume of interest for trabecular bone analysis in the distal femur spanned from 50 µm below the growth plate to the proximal direction, covering a total length of 1.0 mm. Bone mineral density (BMD) and bone morphometric parameters were analyzed using CTAn software (version 1.20.3.0, Bruker).

Jang S.A., Lee S.J., Hwang Y.H, & Ha H. (2023). Anti-Osteoporotic Potential of Water Extract of Anethum graveolens L. Seeds. Nutrients, 15(19), 4302.

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