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Phospho alk y1282 1283

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Phospho-ALK (Y1282/1283) is a laboratory reagent used to detect and quantify the phosphorylation of the Anaplastic Lymphoma Kinase (ALK) protein at tyrosine residues 1282 and 1283. This product is designed for research use only and its core function is to provide a tool for studying the activation state of the ALK protein.

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Lab products found in correlation

Gapdh, by Merck Group (2 mentions) Phosphor s6, by Cell Signaling Technology (1 mentions)

2 protocols using phospho alk y1282 1283

1

Protein Signaling Pathway Analysis

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5×105 cells were treated in 6 well plates for 6 hours with the indicated drugs. Cells protein lysates were prepared as previously described (6 , 11 (link)). Phospho-ERK (T202/Y204), ERK, S6, phosphor S6, phospho-AKT (S473 and T308), AKT, phospho-ALK (Y1282/1283), and ALK antibodies were obtained from Cell Signaling Technology. GAPDH was purchased from Millipore.
Friboulet L., Li N., Katayama R., Lee C.C., Gainor J.F., Crystal A.S., Michellys P.Y., Awad M.M., Yanagitani N., Kim S., Pferdekamper A.C., Li J., Kasibhatla S., Sun F., Sun X., Hua S., McNamara P., Mahmood S., Lockerman E.L., Fujita N., Nishio M., Harris J.L., Shaw A.T, & Engelman J.A. (2014). The ALK inhibitor ceritinib overcomes crizotinib resistance in non-small cell lung cancer. Cancer discovery, 4(6), 662-673.
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2

Immunoblotting for Kinase Phosphorylation

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Immunoblotting method was also used to determine relative kinase phosphorylation status and total protein levels in cells and tumor tissues for the protein of interest. Extracted protein samples from cells or tumor lysates were separated by SDS-PAGE, transferred to nylon membranes, and immunoblotting hybridizations for the proteins of the interest were performed using the corresponding antibodies. Phospho-ERK (T202/Y204), ERK, S6, phospho S6, phospho-AKT (S473 and T308), AKT, phospho-ALK (Y1282/1283), and ALK antibodies were obtained from Cell Signaling Technology. GAPDH was purchased from Millipore.
Zou H.Y., Friboulet L., Kodack D.P., Engstrom L.D., Li Q., West M., Tang R.W., Wang H., Tsaparikos K., Wang J., Timofeevski S., Katayama R., Dinh D.M., Lam H., Lam J.L., Yamazaki S., Hu W., Patel B., Bezwada D., Frias R.L., Lifshits E., Mahmood S., Gainor J.F., Affolter T., Lappin P.B., Gukasyan H., Lee N., Deng S., Jain R.K., Johnson T.W., Shaw A.T., Fantin V.R, & Smeal T. (2015). PF-06463922, an ALK/ROS1 inhibitor, overcomes resistance to 1st and 2nd generation ALK inhibitors in pre-clinical models. Cancer cell, 28(1), 70-81.
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