For c-Fos immunostaining, free-floating tissue sections were obtained and washed with PBS with Triton X-100 (PBS-T) and then blocked with normal donkey serum (5 in 0.1% PBS-T) for 1 h. The sections were subsequently incubated with rabbit polyclonal anti-c-Fos antibody (1:1000 in PBS-T; Santa Cruz Biotechnology, California) for 72 h on a nutating mixer at 4 °C. After incubation with the primary antibody the sections were submerged in Alexa Flour 594-conjugated donkey anti-rabbit secondary antibody (1:500 in PBS-T; Invitrogen) for 90 min at room temperature.
Rabbit polyclonal anti c fos antibody
Manufactured by Santa Cruz Biotechnology
The Rabbit polyclonal anti-c-Fos antibody is a laboratory reagent designed for the detection and analysis of the c-Fos protein. The c-Fos protein is a transcription factor that is part of the Activator Protein-1 (AP-1) complex and plays a role in cellular processes such as differentiation, proliferation, and transformation. This antibody can be used in various immunoassay techniques, including Western blotting, immunohistochemistry, and immunocytochemistry, to identify and quantify the c-Fos protein in biological samples.
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2 protocols using rabbit polyclonal anti c fos antibody
1
Immunostaining Protocol for c-Fos
2
Immunofluorescent Staining of c-Fos
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Mice were perfused and their brains extracted 75 min after initial exposure to the experimental condition. Free-floating tissue sections were collected, washed with PBS containing Triton X-100 (PBST), and blocked with 5% normal donkey serum for one hour. The tissue was later incubated at 4 °C for 72 h with rabbit polyclonal anti-c-Fos antibody (1:1000 in PBST; Santa Cruz Biotechnology, California). Next, the sections were incubated at room temperature with Alexa Flour 594-conjugated (or Alexa Flour 488-conjugated) donkey anti-rabbit secondary antibody (1:500 in PBST; Invitrogen) for 2 h.
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