Glu1 fibrinopeptide b lock mass standard glufib

High purity LC-MS grade water and acetonitrile containing formic acid used for mobile phase preparation, and ammonium bicarbonate reagent were purchased from J.T. Baker (Phillipsburg, NJ). RapiGest™ surfactant and [Glu1]-Fibrinopeptide B lock mass standard (GluFib) were purchased from Waters (Milford, MA). Formic acid, urea, and Zeba™ spin desalting column (7K MWCO) were purchased from Pierce (Rockford, IL). Dithiothreitol (DTT) and iodoacetamide were purchased from ThermoFisher Life Technologies (Grand Island, NY) and Sigma-Aldrich (St. Louis, MO), respectively. Trypsin (modified sequencing grade), chymotrypsin, IdeS (immunoglobulin G-degrading enzyme of Streptococcus pyogenes), and PNGase F glycosidase were purchased from Promega (Madison, WI).

High purity LC-MS grade water and acetonitrile containing formic acid used for mobile phase preparation and ammonium bicarbonate reagent were purchased from J.T. Baker (Phillipsburg, NJ). RapiGest™ surfactant and [Glu1]-Fibrino-peptide B lock mass standard (GluFib) were purchased from Waters (Milford, MA). Formic acid, urea, and Zeba™ spin desalting column (7 K MWCO) were purchased from Pierce (Rockford, IL). Dithiothreitol (DTT) and iodoacetamide were purchased from ThermoFisher Life Technologies (Grand Island, NY) and Sigma-Aldrich (St. Louis, MO), respectively. Trypsin (modified sequencing grade), chymotrypsin, and PNGase F glycosidase and endoglycosidase (Endo) H were purchased from Promega (Madison, WI). Alpha 1–2,3 mannosidase was purchased from New England Biolabs (Ipswich, MA).