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Anti mouse minus probe

Manufactured by Merck Group
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Sourced in Italy

The Anti-Mouse MINUS probe is a laboratory reagent used in various molecular biology and cell biology applications. Its core function is to specifically detect and bind to mouse-derived cellular components, enabling researchers to differentiate between mouse and non-mouse samples or targets. The probe is designed to provide a reliable and sensitive detection method for mouse-specific analytes.

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Lab products found in correlation

Orange detection reagent, by Merck Group (2 mentions) Anti rabbit plus probe, by Merck Group (2 mentions) Hcx plan apochromat 63 1.40 0 60 na oil λ blue objective, by Leica (2 mentions) Lsm 710, by Zeiss (2 mentions) Neon transfection system, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Duolink kit, by Merck Group (1 mentions) Anti rabbit plus, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes Anti-mouse MINUS PLA probes Duolink anti-Mouse MINUS and anti-Rabbit PLUS In Situ PLA probes Duolink® In Situ PLA® Probe Anti-Mouse MINUS In Situ PLA Probe Anti-Mouse MINUS Duolink Probe anti-mouse MINUS PLA probe anti-mouse minus Anti-mouse MINUS

5 protocols using anti mouse minus probe

1

Proximity Ligation Assay for Protein Interactions

Cited 1 time
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Proximity ligation assay (PLA) was performed using the anti-Mouse MINUS probe, anti-Rabbit PLUS probe and the orange detection reagent (Sigma-Aldrich) as previously described(53 (link)). Actin was visualized using actistain 670 and imaged on a Leica scanning confocal (SP5) with a HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective. The total number of distinct PLA spots per cell were quantified using the threshold function of ImageJ.
Goreczny G.J., Forsythe I.J, & Turner C.E. (2018). Hic-5 Regulates Fibrillar Adhesion Formation to Control Tumor Extracellular Matrix Remodeling through Interaction with Tensin1. Oncogene, 37(13), 1699-1713.
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2

Proximity Proteomics of Id2 and Sirt2

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PC12 cells were transfected with si-Id2, -Sirt2 using neon transfection system (Invitrogen) and seeded onto 12 mm glass coverslips in 24-well plates. The cells were fixed with 4% PFA. Primary antibodies used were anti-tubulin (rabbit, cat. 801202), anti-Id2 (mouse, cat. sc-389104), and mouse anti-tubulin (cat. T 2200), anti-Sirt2 (rabbit, cat. S 8447). Following the manufacturer’s protocol, secondary antibodies (anti-rabbit PLUS probe and anti-mouse MINUS probe, Sigma) were incubated with the cells, and following the Duolink protocol, if the two proteins were sufficiently close, rolling circle amplification was triggered by the subsequent additions. Amplified DNA was detected by a specific oligonucleotide that was labeled with a red fluorescent. Cells were analyzed via a confocal microscope (LSM 710, Carl Zeiss, Germany).
Yun T., Ko H.R., Jo D.G., Park K.W., Cho S.W., Kim J, & Ahn J.Y. (2021). Inhibitor of DNA binding 2 (Id2) mediates microtubule polymerization in the brain by regulating αK40 acetylation of α-tubulin. Cell Death Discovery, 7, 257.
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3

Proximity Ligation Assay for Protein Interactions

Cited 1 time
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Proximity ligation assay (PLA) was performed using the anti-Mouse MINUS probe, anti-Rabbit PLUS probe and the orange detection reagent (Sigma-Aldrich) as previously described(53 (link)). Actin was visualized using actistain 670 and imaged on a Leica scanning confocal (SP5) with a HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective. The total number of distinct PLA spots per cell were quantified using the threshold function of ImageJ.
Goreczny G.J., Forsythe I.J, & Turner C.E. (2018). Hic-5 Regulates Fibrillar Adhesion Formation to Control Tumor Extracellular Matrix Remodeling through Interaction with Tensin1. Oncogene, 37(13), 1699-1713.
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4

Proximity Ligation Assay for Protein-Protein and Protein-DNA Interactions

Cited 2 times
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The PLA procedure was described in detail previously (Dai et al., 2015 (link); Su et al., 2016 (link)). Duolink® In Situ PLA® anti-rabbit Plus probes, anti-mouse Minus probes, and Detection Reagents Red and Green were all purchased from Sigma-Aldrich. For detection of AMPK-peptide interactions, rabbit anti-AMPKα1 Abs (1:100 dilution), rabbit anti-AMPKβ1+β2 monoclonal Ab clone E.427.6 (1:100 dilution), or rabbit anti-AMPKγ1+2+3 polyclonal Abs (1:100, dilution) were combined with mouse monoclonal anti-biotin Ab-2 BTN.4 (1:100 dilution). For detection of genomic DNA interactions, rabbit anti-GLI1 Abs (1:200 dilution) or rabbit anti-SREBP1 Abs (1:200 dilution) were combined with mouse monoclonal anti-dsDNA Abs HYB331–01 (1:100 dilution). The detailed procedure was described previously (Dai et al., 2015 (link)). Nuclei were counterstained with Hoechst 33342. PLA signals were documented by a Zeiss LSM780 confocal microscope or quantitated by a BD FACSCalibur™ flow cytometer.
Su K.H., Dai S., Tang Z., Xu M, & Dai C. (2019). Heat Shock Factor 1 Is a Direct Antagonist of AMP-Activated Protein Kinase. Molecular cell, 76(4), 546-561.e8.
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5

Proximity Ligation Assay for IR-RAGE Interaction

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PLA was performed using the Duolink kit (Sigma-Aldrich, Italy) to detect IR and RAGE direct interaction, as recommended by the manufacturer. A rabbit primary antibody against RAGE (D1A12, Cell Signaling Technology, distributed by Euroclone, Italy) and a mouse primary antibody against IR (L55B10, Cell Signaling Technology, distributed by Euroclone, Italy) were used, together with Anti-Rabbit PLUS and Anti-Mouse MINUS probes (PLA probes, Sigma-Aldrich, Italy). PLA signals were detected using a fluorescence microscope TI-E (Nikon, Netherland). More details are available in Supplementary Materials and Methods.
Muoio M.G., Pellegrino M., Rapicavoli V., Talia M., Scavo G., Sergi V., Vella V., Pettinato S., Galasso M.G., Lappano R., Scordamaglia D., Cirillo F., Pulvirenti A., Rigiracciolo D.C., Maggiolini M., Belfiore A, & De Francesco E.M. (2023). RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer. Breast Cancer Research : BCR, 25, 84.
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