Plan fluor 20x dic objective

Manufactured by Nikon

Sourced in Japan

The Plan Fluor 20x DIC objective is a high-quality optical lens designed for use in laboratory equipment. It features a plan-apochromatic correction for superior flatness of field and color correction. The DIC (Differential Interference Contrast) functionality enables enhanced contrast and clarity in sample imaging. This objective is suitable for a wide range of applications requiring high-resolution, detailed observations.

Automatically generated - may contain errors

Lab products found in correlation

6 well plate, by MatTek (2 mentions) Volocity software, by PerkinElmer (1 mentions) A1r confocal laser scanning microscope system, by Nikon (1 mentions) Eclipse ti, by Nikon (1 mentions)

Close spelling variants of this product

Plan Fluor 20x objective Plan Fluor 20X Plan Fluor objective Plan Fluor

2 protocols using plan fluor 20x dic objective

Monitoring Skin Tissue Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microscopic studies of tissue explants were performed as described previously^{53 (link)}. Briefly, skin biopsies were performed 24 hours after the injection auxo-GTU^®-Luc-EGFP plasmid and 2 hours after the in vivo id injection of anti-HLA-DR-AF700 Ab (Ozyme, France). Fat tissue was removed and each biopsy was cut into two equal parts and placed in a 6-well plate (MatTek Corporation, Ashland, USA) in contact with complete culture medium to visualize dermal and epidermal skin layers. Images were acquired with a Plan Fluor 20x DIC objective (NA: 0.45) on a Nikon A1R confocal fast laser scanning system (Nikon Corporation, Japan) equipped with a thermostatic chamber (37 °C; 5% CO₂). Images were recorded with a high-speed resonant scanner every 10 minutes for 22 hours. Volocity software (Perkin Elmer, Waltham, USA) was used to reconstruct three-dimensional images and to assess cell motility (velocity, displacement and confinement ratio).

Todorova B., Adam L., Culina S., Boisgard R., Martinon F., Cosma A., Ustav M., Kortulewski T., Le Grand R, & Chapon C. (2017). Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques. Scientific Reports, 7, 4122.

+ Open protocol

+ Expand

In Vivo Localization of Fluorescent Immune Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human anti-CD207 mAb coupled to HIV-gag protein, its control isotype IgG4-gag, and the Gag protein, were provided by the Baylor Institute for Immunology Research (BIIR, Dallas). Fluorescent labeling of these fusion proteins and the anti-HLA-DR mAb (clone L243, Ozyme, St Quentin en Yvelines, France) was performed using respectively Fluoprobe 682 (F682) and 490 (F490) kits (Interchim, Montluçon, France). Ten μg of IgG4-Gag-F682 mAb, anti-Lang-Gag-F682 mAb, or Gag-F682 protein were injected i.d. with 10 μg of anti-HLA-DR-F490 mAb in 100 μl of PBS solution in adult NHP under anesthesia. Biopsies at the injection sites were taken removed 2 hours after in vivo i.d. injection of fluorescent mAbs. Each skin biopsy was placed in a 6-well plate (MatTek Corporation, Ashland, MA, USA) in contact with RPMI-1640 containing 100 μg/ml of Penicillin/Streptomycin/Neomycin and 5% FCS to analyze dermis and epidermis. Fluorescent images were captured through a Plan Fluor 20x DIC objective (NA: 0.45) on a Nikon A1R confocal laser scanning microscope system attached to an inverted ECLIPSE Ti (Nikon Corporation, Tokyo, Japan) held at 37°C under a 5% CO2 atmosphere.

Epaulard O., Adam L., Poux C., Zurawski G., Salabert N., Rosenbaum P., Dereuddre-Bosquet N., Zurawski S., Flamar A.L., Oh S., Romain G., Chapon C., Banchereau J., Lévy Y., Le Grand R, & Martinon F. (2014). Macrophage- and Neutrophil-Derived TNF-α Instructs Skin Langerhans Cells to Prime Antiviral Immune Responses. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2416-2426.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!