Au480 analyzer

Manufactured by Beckman Coulter

Sourced in United States, Germany, Switzerland

The AU480 analyzer is a fully automated clinical chemistry analyzer designed for high-volume clinical laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests. The AU480 analyzer utilizes proven technology to provide accurate and reliable results.

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Lab products found in correlation

K2edta, by BD (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Formic acid solution, by Merck Group (1 mentions) Direct q water purification system, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Lichropur, by Merck Group (1 mentions) Lichrosolv, by Merck Group (1 mentions) P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) P p38 t180 y182, by Cell Signaling Technology (1 mentions) Psfks y416, by Cell Signaling Technology (1 mentions) Pyapy357, by Abcam (1 mentions) Ecl plus system, by Thermo Fisher Scientific (1 mentions) Cxcl2 elisa kit, by R&D Systems (1 mentions) P stat3 y705, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) β actin, by Abcam (1 mentions) Allegra x 12r, by Beckman Coulter (1 mentions)

29 protocols using au480 analyzer

Serum and Urinary Calcium Evaluation

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Initial preprandial blood and urine samples were submitted immediately upon collection to the VITALS Laboratory of the Virginia Maryland College of Veterinary Medicine for a serum biochemistry profile and urinalysis. If the urine dipstick detected ≤2+ proteinuria, then a sulfosalicylic acid precipitation (SSA) test was performed. If this test was also positive, then a urine protein to creatinine ratio was calculated. If the serum total calcium concentration was outside the laboratory reference range, then a serum ionized calcium concentration was determined.
The baseline and postprandial urine samples were submitted immediately for calcium and creatinine measurements. Urine calcium and creatinine concentrations were measured by spectroscopy on all 5 samples using a Beckman Coulter AU 480 analyzer, using the calcium‐sensitive Arsenazo dye and the modified Jaffe procedures, respectively.

Carr S.V., Grant D.C., DeMonaco S.M, & Shepherd M. (2020). Measurement of preprandial and postprandial urine calcium to creatinine ratios in male Miniature Schnauzers with and without urolithiasis. Journal of Veterinary Internal Medicine, 34(2), 754-760.

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Tissue Sampling and Plasma Analysis

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Animals were killed by cervical dislocation. Hearts were removed, frozen in liquid nitrogen and stored at -80°C. Blood was collected by cardiac puncture exsanguination with K2-EDTA (Becton Dickinson Franklin Lakes, NJ, USA) and plasma separated by centrifugation at 10 000 rcf for 4 min at 4°C. Plasma transaminases were measured using a Beckman Coulter AU480 analyzer. Tissues were collected, weighed, flash frozen on liquid nitrogen and stored at -80°C.

Radke M.H., Badillo-Lisakowski V., Britto-Borges T., Kubli D.A., Jüttner R., Parakkat P., Carballo J.L., Hüttemeister J., Liss M., Hansen A., Dieterich C., Mullick A.E, & Gotthardt M. (2021). Therapeutic inhibition of RBM20 improves diastolic function in a murine heart failure model and human engineered heart tissue. Science translational medicine, 13(622).

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Plasma BUN Levels in Saa3-promoter luc Mice

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The Plasma BUN levels of Saa3-promoter luc mice induced with two moderate doses of STZ were determined using a Beckman Coulter AU480 analyzer (Beckman Coulter, Krefeld, Germany), which is an automated chemistry instrument for turbidimetric, spectrophotometric, and ion-selective electrode measurements.

Saliu T.P., Yazawa N., Hashimoto K., Miyata K., Kudo A., Horii M., Kamesawa M., Kumrungsee T, & Yanaka N. (2022). Serum Amyloid A3 Promoter-Driven Luciferase Activity Enables Visualization of Diabetic Kidney Disease. International Journal of Molecular Sciences, 23(2), 899.

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Quantitative Analysis of Drugs in Urine and Hair

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Standards and deuterated internal standards were purchased from Cerilliant (Round Rock, TX, USA). Working solutions for preparing calibration samples for urine and hair analysis were obtained via serial dilution of the standard solution in Acetonitrile. Acetonitrile (99.9%, HPLC gradient grade) was acquired from Acros Organics (Chemie Brunschwig, Basel, Switzerland). Ammonium hydroxide solution (25%), methanol, dichloromethane (all EMSURE^®), and acetone (LiChrosolv^®) were obtained from Merck (Grogg Chemie, Stettlen, Switzerland). Formic acid solution (puriss p.a., 50% in water) and ammonium formate (LiChropur^®) were from Sigma-Aldrich (Buchs, Switzerland). Ultrapure water was generated in-house with a Direct-Q water purification system from Millipore (Zug, Switzerland). Drug-free urine and hair samples were donated by employees of the authors’ institute. Urine creatinine concentrations were determined spectrophotometrically on an AU480 analyzer (Beckman Coulter, Nyon, Switzerland) using the Jaffe method [26 ].

Haedener M., Weinmann W., Eich D., Liebrenz M., Wuethrich T, & Buadze A. (2021). Evaluating the reliability of hair analysis in monitoring the compliance of ADHD patients under treatment with Lisdexamphetamine. PLoS ONE, 16(3), e0248747.

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Plasma Biomarkers Analysis in Guatemala

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In addition to the birth village and date of birth, the original study collected data on maternal characteristics such as age, height, and schooling years. In the 2015 to 2017 wave of data collection, we obtained data on participant characteristics such as years of schooling, socioeconomic (SES) status, residence in Guatemala City, and self-reported alcohol intake. Trained field workers and phlebotomists collected anthropometric measurements and fasting blood samples, as previously described ^{27 (link)}.
Plasma samples were frozen for storage at −80°C until being shipped on dry ice to a biomarker core laboratory in the United States. The samples were thawed at 4°C in batches, each containing approximately 40 plasma samples. Plasma ALT and AST values were assessed using the AU480 analyzer (Beckman Coulter Diagnostics, Fullerton CA, US) using enzymatic methods (Sekisui Diagnostics P.E.I. Inc., Canada). Insulin was assayed using immunoturbidimetric methods (Kamiya Biomedical Company, WA, US).

Sekkarie A., He S., Welsh J.A., Ramakrishnan U., Stein A.D, & Vos M.B. (2020). Prevalence of NAFLD in Guatemala following exposure to a protein-energy nutrition intervention in early life. Annals of hepatology, 19(4), 373-379.

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Inflammatory Biomarkers: ESR and CRP

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Erythrocyte Sedimentation Rate (ESR) and serum C—reactive protein (CRP) levels also confirmed the inflammatory status of control. The samples collected from the control and patients were measured for ESR by the Westergren method following the procedure described previously [23 (link)]. The CRP levels in the serum of controls were analyzed by using an immuno-turbidimetric assay kit (CRP Turbilatex, Beacon, NY, USA) using an AU480 analyzer (Beckman Coulter, Brea, CA, USA).

Yadav S.R., Goyal B., Mamgain G., Kothari A., Kumar S., Saha S., Naithani M., Mirza A.A., Kumar R, & Arora R. (2023). Genetic Variations in IL-1β, TNF-α, and TGF-β Associated with the Severity of Chronic Cervical Spondylitis in Patients. Cells, 12(12), 1594.

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Comprehensive Metabolic Panel Analysis

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Serum alanine transaminase (ALT), aspartate transaminase (AST), cholesterol (T-CHOL), LDL- cholesterol (LDL-C), serum urea nitrogen (BUN), and ketone body and triglyceride (TAG) levels were measured using AU480 analyzer (Beckman Coulter, Krefeld, Germany), which is an instrument for turbidimetric, spectrophotometric and ion-selective electrode measurements.

Aisyah R., Ohshima N., Watanabe D., Nakagawa Y., Sakuma T., Nitschke F., Nakamura M., Sato K., Nakahata K., Yokoyama C., Marchioni C.R., Kumrungsee T., Shimizu T., Sotomaru Y., Takeo T., Nakagata N., Izumi T., Miura S., Minassian B.A., Yamamoto T., Wada M, & Yanaka N. (2024). GDE5/Gpcpd1 activity determines phosphatidylcholine composition in skeletal muscle and regulates contractile force in mice. Communications Biology, 7, 604.

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Multimodal Protein Analysis in Hepatic Disease

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Western blot analyses were performed using standard procedures with the ECL Plus system (Thermo Fisher Scientific). Antibodies targeting the following proteins were used: p-YAP (Y357) (Abcam, ab62751), YAP (Cell Signaling Technology, 4912), p-STAT3 (Y705) (Cell Signaling Technology, 9145), STAT3 (Cell Signaling Technology, 12640), p-SFKs (Y416) (Cell Signaling Technology, 2101), SRC (Thermo Fisher Scientific, AHO1152), Yes (Cell Signaling Technology, 65890), AKT1 (Cell Signaling Technology, 2938), p-AKT (S473) (Cell Signaling Technology, 4060), p38 (Cell Signaling Technology, 8690), p-p38 (T180/Y182) (Cell Signaling Technology, 4511), ERK1/2 (Cell Signaling Technology, 4695), p-ERK1/2 (T202/Y204) (Cell Signaling Technology, 4370), CCN1 (R&D Systems, AF4055), and β-actin (Abcam, ab8226) were detected by the antibodies. The intrahepatic IL-6 and CXCL2 levels were analyzed in the whole liver lysates by using an IL-6 ELISA kit (eBioscience) and CXCL2 ELISA kit (R&D Systems). The serum levels of ALT and AST were measured by the Biological Resources facility at the University of Illinois at Chicago using a Beckman Coulter AU480 analyzer.

Cheng N., Kim K.H, & Lau L.F. (2022). Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2. JCI Insight, 7(14), e158207.

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Serum Biochemical Analysis from Mice

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Serum was prepared from whole blood collected from young adult female offspring via cardiac puncture. Biochemical analysis of serum samples was conducted at the Toronto Centre for Mouse PhenoGenomics at the Hospital for Sick Children in Toronto using a Beckman Coulter AU480 analyzer.

Burke A.P., Niibori Y., Terayama H., Ito M., Pidgeon C., Arsenault J., Camarero P.R., Cummins C.L., Mateo R., Sakabe K, & Hampson D.R. (2018). Mammalian Susceptibility to a Neonicotinoid Insecticide after Fetal and Early Postnatal Exposure. Scientific Reports, 8, 16639.

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Serum Metabolite Analysis Protocol

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Blood was centrifuged (Beckman Coulter Allegra X-12R, Centrifuge) at 2,000 × g for 20 min. Serum separated was collected and analyzed for different metabolites (Table 2) at Oregon State Veterinary Diagnostic Laboratory where samples were run on an AU480 analyzer (Beckman Coulter, Brea, CA).

Cherian G., Fraz A, & Bionaz M. (2023). Evaluating the impact of organic chromium on hepatic phospholipid fatty acid molecular species, transcription of genes associated with lipid metabolism and oxidative status in broiler chickens fed flaxseed. Poultry Science, 102(10), 102976.

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