Pc 16 0 16 0

Imidazolium amphiphile IA-12 was synthesized in accordance with the standard experimental procedure [45 (link),49 (link)]. Commercially available double-stranded oligonucleotide (oNu) with 10 nucleotide units in each strand (GCGTTAACGC, molecular weight is 3028 g/mol, Joint Stock Company Syntol, Moscow, Russia) was used. Plasmid DNA (pDNA) pVax1-DsRed (3665 bp) was kindly gifted by Dilara Gatina (Omics Technology Laboratory, Kazan Federal University, Kazan, Russia). Agarose for molecular biology, ethidium bromide and hexadecyltrimethylammonium bromide (≥99.0%) were purchased from Sigma-Aldrich. As probes for fluorometric measurements, ethidium bromide (Sigma-Aldrich, St. Louis, MO, USA, 95%) and pyrene (Sigma-Aldrich, St. Louis, MO, USA, 99%) were used. Lipoid PC 16:0/16:0 (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) was a gift from Lipoid GmbH (Ludwigshafen, Germany). Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA, 99%) was applied for the fluorescence microscopy assay. Polyacrylic acid with an average molecular weight of 1800 g/mol (Sigma-Aldrich, St. Louis, MO, USA, 99%) was used. Polymer concentrations were given as a molar concentration on a monomer basis. Purified water (18.2 MΩ cm resistivity at 25 °C) from Direct-Q 5 UV equipment (Millipore S.A.S. 67120 Molsheim-France) was used for all solution preparation.

Black Phosphorus (bulk) was purchased from Ossila Ltd., Sheffield, UK (Product Code M2106C1). All chemicals were purchased from Sigma-Aldrich, Gillingham, UK. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, (Lipoid PC 16:0/16:0 (DPPC), 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, (DOPE Lipoid 565600), and [N-(Carbonyl-methoxy polyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethano[amine, sodium salt]] (DSPE-PEG2000 Lipoid 588200) were generous gifts from Lipoid GmbH (Ludwigshafen, Germany). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (MSPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). Doxorubicin hydrochloride (Dox·HCl) was obtained from Biosynth Ltd., Compton, UK (Product Code: AD15377). Roswell Park Memorial Institute (RPMI) 1640 Medium, Dulbecco’s PBS (1x), penicillin–streptomycin solution liquid (10,000 units/mL), glutamine supplement (200 mM), and 0.25% trypsin/EDTA were purchased from ThermoFisher Scientific, Winsford, UK. Heat-inactivated foetal bovine serum (FBS) (11580516) was obtained from Gibco Life Technologies, Paisley, UK.