Opmi vario s88

Manufactured by Zeiss

Sourced in Germany

The OPMI Vario S88 is a high-performance surgical microscope developed by Zeiss. It features a variable zoom optic system that provides a wide range of magnification options to support various surgical procedures. The microscope is designed to deliver precise, high-quality imaging to facilitate accurate visualization and examination during complex operations.

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Lab products found in correlation

Ciloxan, by Alcon (1 mentions) Lacri lube, by Abbvie (1 mentions) Maxidex, by Alcon (1 mentions) Hopkins telescope, by Storz (1 mentions) Axioplan 2 imaging, by Zeiss (1 mentions)

8 protocols using opmi vario s88

Microsurgical Suturing Training Protocol

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The training task board was designed iteratively by experienced surgeons in collaboration between 2 intercontinental university hospitals. The task cardboard had 2 rows with 3 slots each, in total 6 slightly different suturing subtasks. Each column has predefined direction (0 – 45 – 90–degree angles), and the second row repeats them under higher magnification. To each incision, the participants completed 2 sutures. The card is designed to intuitively force the performer to adjust for different magnifications and field of view, which are typically encountered in microsurgical suturing.
The participants conducted the sutures using high-quality microsurgical needle holders and suturing forceps with 9.3 mm 3/8 taper head needles attached to 7-0, 50-cm polypropylene monofilament sutures. The participants used a Zeiss OPMI Vario S88 surgical microscope with an embedded custom-made eye tracker. The eye tracker had a sampling rate of 30 Hz and was installed on the right ocular of the microscope. Figure 1 shows the scene under the microscope and the view from the eye tracker.Figure 1.

(A) Scene under the microscope and (B) the eye during a blink.

Koskinen J., Bednarik R., Vrzakova H, & Elomaa A.P. (2020). Combined Gaze Metrics as Stress-Sensitive Indicators of Microsurgical Proficiency. Surgical Innovation, 27(6), 614-622.

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Whisker Deprivation and Physiological Effects

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At the postnatal 60 (P60) day, the whisker-deprived group was intraperitoneally anesthetized with ketamine (10 mg/kg) plus xylazine (5 mg/kg) and the facial vibrissae were fulgurated under a surgical microscope (OPMI Vario/S88, Carl Zeiss Germany). Briefly, each whisker was gently pulled out and ablated with a surgical cautery device. The control group received the same pharmacological manipulation, but no whisker removal procedure was done. After that, the animals were placed in a pre-warmed cage until recovery. We quantified the bodyweight gain every 2 days and corroborated the fact that facial injuries healed in less than 72 h.

Gonzalez-Perez O., López-Virgen V, & Ibarra-Castaneda N. (2018). Permanent Whisker Removal Reduces the Density of c-Fos+ Cells and the Expression of Calbindin Protein, Disrupts Hippocampal Neurogenesis and Affects Spatial-Memory-Related Tasks. Frontiers in Cellular Neuroscience, 12, 132.

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Chronic Glaucoma Model in Rats

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A chronic model of experimental glaucoma was generated in rats by a producing an increase of IOP using microbead injections (Fluospheres, 10 μm) as described previously^{2 (link)}. Weekly injections into the anterior chamber of eye were made for a sustained increase in IOP for 2 months following which the tissues were harvested. Microbeads were administered using a 25-μL syringe connected to a disposable 33-gauge needle (TSK Laboratory, Japan). All injections were performed under an operating microscope (OPMI Vario S88, Carl Zeiss, Germany). Care was taken to avoid needle contact with the iris or lens and minimise damage. The needle was inserted beneath the corneal surface and 5 μL of beads were injected. Rodents were anesthetized using ketamine (75 mg/kg) and medetomidine (0.5 mg/kg) intraperitoneally^{69 (link)}, and anaesthesia reversed using atipamazole (0.75 mg/kg injection. 0.3% Ciprofloxacin drops (0.3%) (Ciloxan; Alcon Laboratories, Australia) and 0.1% dexamethasone eye drops (Maxidex, Alcon Laboratories) were instilled in the eyes. Lacrilube; (Allergan) was applied to cornea to protect the rodent eyes. IOP was regularly measured with the help of rebound tonometer (Icare Tonovet, Finland). Three consecutive pressure readings were recorded from the eyes and their average taken to determine the intraocular pressure.

Gupta V., Mirzaei M., Gupta V.B., Chitranshi N., Dheer Y., Vander Wall R., Abbasi M., You Y., Chung R, & Graham S. (2017). Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Scientific Reports, 7, 8412.

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Tympanoplasty with Cholesteatoma Removal

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For each of the patients, tympanoplasty was performed via a postauricular approach under a microscope (OPMI^® Vario/S88, ZEISS, Germany). Mastoidectomy was performed for all patients with cholesteatoma and the cholesteatoma was removed by canal wall up tympanoplasty. An endoscope (HOPKINS^®Telescope, KARL STORZ, Germany) was used to confirm that there was no residual cholesteatoma post-surgery. For the adhesive otitis media cases, the tympanic membrane adhered to the tympanic cavity was peeled off and the adhered pathological tympanic membrane was removed. For ossicular reconstruction, columella on the stapes were performed in patients whose stapes superstructure remained, while columella on the foot plate was performed in patients whose stapes superstructure had been lost.

Yamamoto K., Yamato M., Morino T., Sugiyama H., Takagi R., Yaguchi Y., Okano T, & Kojima H. (2017). Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation. NPJ Regenerative Medicine, 2, 6.

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Extraction and Structural Analysis of Human Otoconia

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Human otoconia were extracted from patients undergoing transmastoid labyrinthectomy for sporadic vestibular schwannoma as described recently [14] (link). Human utricles were identified and extracted with the maximum magnification of a surgery microscope (OPMI Vario/S 88 Carl Zeiss, Oberkochen, Germany) after removing the bony structures from the semicircular canals and the vestibule. Specimens were harvested after cutting out endolymphatic tissue with a beaver knife and were immediately fixed in ethanol (96%) for further structural investigations.
Samples lying in the gelatinous matrix were identified by light microscopy (Axioplan 2 imaging, Carl-Zeiss, Oberkochen, Germany) with 300-fold magnifications. After that, the samples were transferred to conductive (polycarbonate/graphite) foil discs (G3347, FEI/Philips) for investigations by ESEM. Groups of intact human otoconia as well as of otoconia in earlier stages of degeneration were identified at higher ESEM magnifications (up to >1:40,000). For investigations under high vacuum (HV) modes (2×10⁻⁴ Pa), some samples of human otoconia were coated with gold (Au) in order to obtain a reliable conductivity of the surface. Human otoconia were treated with hydrochloric acid, EDTA, demineralized water and completely purified water respectively. Monitoring of structural changes by ESEM was performed as described for artificial otoconia.

Walther L.E., Blödow A., Buder J, & Kniep R. (2014). Principles of Calcite Dissolution in Human and Artificial Otoconia. PLoS ONE, 9(7), e102516.

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Laser Cordectomy for Glottic Lesions

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All procedures were performed with the patient under general anesthesia. Suspension laryngoscopy was performed, and the lesion was examined under microscopy (OPMI Vario S88; Zeiss). Cordectomy type Va or III, according to the European Laryngological Society classification, 15 was performed using CO 2 laser with an AcuSpot 712 Micromanipulator (Lumenis AcuPulse 30/40W; Adisat), considering an adequate safety margin when it was at least 2 mm from the tumor. When required for adequate glottic exposure, a partial vestibulectomy or Burp maneuver was performed. The surgical specimen was fixed to a soft board, with the margins marked with ink and sent for definitive analysis by an experienced surgical pathologist. No frozen sections were performed.

Cabrera-Sarmiento J.A., Vázquez-Barro J.C., González-Botas J.H., Chiesa-Estomba C, & Mayo-Yáñez M. (2021). T1b Glottic Tumor and Anterior Commissure Involvement: Is the Transoral CO₂ Laser Microsurgery a Safe Option?. Ear, nose, & throat journal, 100(1_suppl).

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NMDA-Induced Retinal Neurotoxicity in Mice

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Retinal neurotoxicity was induced by a single intravitreal injection of NMDA (30 nmol per eye) (Dheer et al., 2019). Mice were anesthetized in an induction chamber with 2–5% isoflurane in oxygen, and then animals were maintained on 1–3% isoflurane in oxygen (0.6–1 L/min flow of oxygen) on a warming pad during the procedure. After pupils were dilated with 1% tropicamide, a single amount of 3 μL NMDA (Sigma-Aldrich, St. Louis, MO, USA) solution in sterile PBS was administered using a 33G needle into the vitreous chamber behind the lens. The control animal group eyes were injected with an equivalent volume of sterile PBS. All injections were performed under an operating surgical microscope (OPMI Vario S88, Carl Zeiss). At 7 days after NMDA injection, inner retinal electrophysiological recordings were performed by measuring pSTR responses, and animals were sacrificed by cervical dislocation to harvest the tissues for analysis.

Basavarajappa D., Gupta V., Chitranshi N., Wall R.V., Rajput R., Pushpitha K., Sharma S., Mirzaei M., Klistorner A, & Graham S.L. (2022). Siponimod exerts neuroprotective effects on the retina and higher visual pathway through neuronal S1PR1 in experimental glaucoma. Neural Regeneration Research, 18(4), 840-848.

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Fasciculus Arcuatus Tractus Dissection

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We analyzed 50 MRI studies in DTI sequences (100 cerebral hemispheres) of patients of the National Institute of Neurology and Neurosurgery (INNN) from January 2018 to May 2019. The images were obtained from a General Electric resonator 1.5T, where FAT was delimited by fibertracking at a BrainLab Workstation. Informed consent was requested from each patient regarding the use of their imaging study. The origin, course, termination, and communication to the contralateral hemisphere were observed. On the other hand, five brains (10 cerebral hemispheres) were fixed in 10% formalin per 2 months and then frozen at -7° for 2 weeks as described by Klingler in the experimental neurosurgery laboratory of the Hospital Civil de Guadalajara “Fray Antonio Alcalde,” where the FAT was dissected.[1 (link)] Its origin, course, and termination were compared and its relationship with the upper longitudinal fascicle, the claustrocortical fibers, and its passage through the superior periinsular sulcus. Microsurgical techniques dissection was performed under ×6 to ×40 magnifications provided by a Zeiss Surgical Microscope (Carl Zeiss OPMI^® Vario S88 Oberkochen, Germany). Fiber tracts were dissected with homemade wooden sticks, microdissectors, and fine forceps.

Marian-Magaña R., González-González A.C., Miranda-García L.A., Villanueva-Solórzano P., González-González M.E., Mejía-Pérez S.I, & Nuñez-Velasco S. (2022). Frontal aslant tract: Anatomy and tractography description in the Mexican population. Surgical Neurology International, 13, 349.

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