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Alexa fluor conjugated anti mouse ha tag or anti rabbit flag tag secondary antibodies

Manufactured by Thermo Fisher Scientific
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Alexa-Fluor conjugated anti-mouse (HA tag) or anti-rabbit (Flag tag) secondary antibodies are fluorescently labeled reagents used to detect the presence of specific target proteins in a sample. These antibodies bind to the primary antibodies that recognize the HA or Flag epitope tags, allowing for visualization and quantification of the tagged proteins through fluorescence detection.

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Lab products found in correlation

8 well chamber glass slide, by Ibidi (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Mitotracker deep red, by Thermo Fisher Scientific (2 mentions) Anti flag, by Merck Group (2 mentions) Anti ha, by BioLegend (2 mentions)

Close spelling variants of this product

Alexa Fluor antibodies (80 citations) Alexa secondary antibodies (41 citations) FLAG (30 citations) Mouse anti-HA (30 citations) Rabbit anti-HA (27 citations) Anti-HA tag (15 citations) HA Tag (11 citations) Mouse anti-Flag (10 citations) Rabbit anti-FLAG (9 citations) Anti-rabbit antibodies (8 citations)

2 protocols using alexa fluor conjugated anti mouse ha tag or anti rabbit flag tag secondary antibodies

1

Subcellular Localization of DdCBE Monomers

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were transfected with a total of 1 ug of plasmid DNA (500 ng for each monomer) to express left (HA-tagged) or right (FLAG-tagged) monomers of each DdCBE using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol. After 24 h incubation, cells were labelled with MitoTracker Deep Red (Thermo Fisher) at a final concentration of 100 nM for 30 min at 37 °C, 5% CO2 incubator. Cells were then seeded on an 8-well chamber glass slide (Ibidi) and fixed in 4% paraformaldehyde/PBS for 15 min at room temperature. Next, cells were washed twice with PBS and permeabilized in PBS containing 0.1% saponin and 1% BSA for 30 min at room temperature. Cells were then immunostained with anti-HA (Biolegend) or anti-FLAG (Sigma Aldrich), followed by Alexa-Fluor conjugated anti-mouse (HA tag) or anti-rabbit (Flag tag) secondary antibodies (Thermo Fisher). Images were taken using a 60× objective with the high-resolution widefield Nikon system. Acquired images were processed in Fiji48 (link).
Mok B.Y., de Moraes M.H., Zeng J., Bosch D.E., Kotrys A.V., Raguram A., Hsu F., Radey M.C., Peterson S.B., Mootha V.K., Mougous J.D, & Liu D.R. (2020). A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature, 583(7817), 631-637.
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2

Subcellular Localization of DdCBE Monomers

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were transfected with a total of 1 ug of plasmid DNA (500 ng for each monomer) to express left (HA-tagged) or right (FLAG-tagged) monomers of each DdCBE using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol. After 24 h incubation, cells were labelled with MitoTracker Deep Red (Thermo Fisher) at a final concentration of 100 nM for 30 min at 37 °C, 5% CO2 incubator. Cells were then seeded on an 8-well chamber glass slide (Ibidi) and fixed in 4% paraformaldehyde/PBS for 15 min at room temperature. Next, cells were washed twice with PBS and permeabilized in PBS containing 0.1% saponin and 1% BSA for 30 min at room temperature. Cells were then immunostained with anti-HA (Biolegend) or anti-FLAG (Sigma Aldrich), followed by Alexa-Fluor conjugated anti-mouse (HA tag) or anti-rabbit (Flag tag) secondary antibodies (Thermo Fisher). Images were taken using a 60× objective with the high-resolution widefield Nikon system. Acquired images were processed in Fiji48 (link).
Mok B.Y., de Moraes M.H., Zeng J., Bosch D.E., Kotrys A.V., Raguram A., Hsu F., Radey M.C., Peterson S.B., Mootha V.K., Mougous J.D, & Liu D.R. (2020). A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature, 583(7817), 631-637.
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